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Injection Guide
Testicular tetrapeptide bioregulator (Lys-Glu-Asp-Gly) that normalizes HPG axis signaling through DNA minor groove binding at testicular gene promoters, increases testosterone synthesis by restoring Leydig cell function, and modulates neuroendocrine feedback loops controlling gonadotropin secretion in aging males
Route
Sublingual (enteric-coated capsules)
Sites
1 recommended
Frequency
Once daily
Preparation
Testagen in its prescribed form
Clean, dry storage container
Measuring device if applicable (oral syringe, measuring cup)
Calendar or reminder app for dosing schedule
Pro Tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example Calculation
Testagen comes in pre-measured doses or forms. Follow the exact dosing instructions on your prescription label. No reconstitution or mixing is typically required for this formulation.
Dose Calculation
Use Testagen exactly as prescribed. Each unit contains the labeled amount. Your healthcare provider will determine the appropriate dose based on your individual needs and response.
Pro Tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Not applicable—this is not an injectable formulation
Follow your provider's instructions for this injection site. Rotate sites regularly to prevent tissue damage.
Rotate between 1 sites to prevent tissue buildup and ensure consistent absorption.
Pro Tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by Step
Follow the specific administration instructions for your Testagen formulation
Take or apply as directed by your healthcare provider
Store properly between uses according to package instructions
Pro Tip
This peptide uses testagen is administered sublingual (enteric-coated capsules)—no injection required. Inject at a 90 degree angle into the muscle. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal Timing
Best time
Take Testagen at the same time each day for consistent blood levels. Morning dosing with breakfast is often preferred, but follow your healthcare provider's specific instructions.
With food?
Testagen can typically be taken with or without food. Taking it with a light meal may help reduce any GI discomfort. Avoid taking with grapefruit juice or high-fat meals unless specifically directed.
Stacking notes
Testagen should be used as directed by your healthcare provider. If combining with other medications or supplements, discuss potential interactions with your provider. Avoid combining with compounds that have overlapping mechanisms unless specifically guided by a medical professional.
Sample Daily Schedule
As prescribed (once daily)
As prescribed by your healthcare provider injection
Site: Sublingual (enteric-coated capsules)—rotate sites if applicable
Maintain a consistent schedule for optimal results with Testagen. Set reminders if needed. If you miss a dose, follow your healthcare provider's instructions—do not double up on doses to compensate.
Dosing Tiers
Dose
1-2 capsules (5 mg each) orally once daily
Frequency
Once daily
Duration
10 days initial assessment
Begin with oral capsule administration. Take on an empty stomach 15-30 minutes before meals. The KEDG tetrapeptide is designed for oral bioavailability. Effects on testosterone are gradual — HPG axis normalization takes weeks. Baseline testosterone levels should be documented before starting. Monitor for any changes in energy, libido, or mood.
Dose
5-10 mg subcutaneously or 2 capsules orally once daily
Frequency
Once daily
Duration
10-20 days per treatment course
Standard protocol as used in the 36-patient clinical trial. Administer once daily for 10-20 consecutive days. Repeat courses every 3-6 months for sustained hormonal support. Can be combined with Prostamax for comprehensive male reproductive health or Epithalon for systemic geroprotection. Testosterone improvements typically become measurable 2-4 weeks after initiating treatment.
Dose
10 mg subcutaneously once daily
Frequency
Once daily
Duration
20 days per course
Extended protocol for significant testicular function decline. Used in comprehensive multi-peptide bioregulator programs combining Testagen with Prostamax (prostate), Epithalon (pineal), Vilon (immune), and Ventfort (vascular) for whole-body male geroprotection. Repeat every 3-6 months. Regular testosterone monitoring recommended. Medical supervision strongly advised.
Preservation
Before Mixing
Store Testagen in the refrigerator at 36-46°F (2-8°C) in its original packaging. Protect from light and moisture. Do not freeze. Check the expiration date before use. Some formulations may be stored at room temperature for limited periods—check your specific product labeling.
After Mixing
Once reconstituted, Testagen should be kept refrigerated at 36-46°F (2-8°C) and used within the timeframe specified on your product labeling (typically 14-28 days). Label the vial with the reconstitution date. Do not use if the solution appears cloudy, discolored, or contains particles.
Shelf Life After Mixing
14 days refrigerated (reconstituted); capsules stable for 2-3 years at room temperature; lyophilized powder 2-3 years at -20°C
Signs of Degradation
Discard the vial immediately if you notice any of these:
Solution appears cloudy, discolored, or contains visible particles (should be clear)
Product has been exposed to temperatures outside the recommended storage range
Product has been frozen (unless specifically designed for freeze-thaw stability)
Expiration date has passed or reconstituted solution has exceeded its use-by date
Unusual odor, color change, or visible contamination
Important
When to Stop
Severe or worsening side effects that don't improve with dose adjustment or supportive care
Signs of an allergic reaction—rash, hives, swelling, or difficulty breathing
Your healthcare provider recommends discontinuation based on your clinical response
Development of any new medical condition that may be contraindicated with Testagen
Pregnancy or planning to become pregnant (unless specifically approved for use during pregnancy)
Abnormal lab results or clinical markers that suggest adverse effects
Testagen should only be started, adjusted, or discontinued under medical supervision. This information is for educational purposes only and does not replace professional medical advice. Never stop a prescribed treatment without consulting your healthcare provider first, as abrupt discontinuation may have consequences.
Clean Technique Checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above