Epithalon
L-alanyl-L-glutamyl-L-aspartyl-glycine
Epithalon is a synthetic tetrapeptide consisting of four amino acids (Ala-Glu-Asp-Gly) developed by Khavinson and colleagues in Russia during the late 1980s. With a molecular weight of 390.35 g/mol, this compact peptide mimics a segment of the naturally occurring thymic peptide epithalamin extracted from pineal glands, making it a valuable tool for research into telomere elongation, pineal gland function, and cellular aging processes in controlled experimental settings.
Epithalon Dosing Guide
Primary Benefits
- Telomerase activity increase - 30-50% enhancement in cellular studies
- Lifespan extension - 12-17% increase in aging animal models
- Tumor reduction - 25-30% decrease in cancer incidence
- Melatonin production boost - 20-25% increase in pineal function
- Enhanced cellular division capacity - 10-15 additional cell cycles
How It Works
Epithalon likely activates telomerase in cell cultures, lengthening telomeres, and may enhance pineal melatonin synthesis in animal models, influencing circadian and HPA responses. The peptide appears to upregulate telomerase activity through unknown pathways, extending cellular replicative lifespan by preventing telomere shortening during cell division. Additionally, it modulates pineal gland function, potentially increasing melatonin production and regulating circadian rhythms, while also influencing the hypothalamic-pituitary-adrenal axis to reduce age-related stress responses.
Quick Reference
Typical Dosage Range
Research protocols employ cyclic dosing: 1 µg/day SC for 5 days monthly over 6 months in mice for longevity studies (Anisimov et al., 2003), 5 µg/day IP for 10 days in rats for pineal function research (Khavinson et al., 2001), and 10 µM for 72 hours in cell culture studies for telomerase activation (Khavinson et al., 2003). All doses are reconstituted in sterile saline at 1 mg/mL concentration.
Administration Methods
Epithalon is administered via subcutaneous (SC) or intraperitoneal (IP) injection in animal studies, reconstituted in sterile saline at 1 mg/mL under aseptic conditions. Cyclic administration protocols (5 days on, 25 days off) are commonly used to mimic physiological pulsatile release patterns. Storage at 2-8°C or -20°C in low-protein-binding vials prevents degradation. Injection timing may align with circadian rhythms for optimal pineal effects.
What It's Used For
Epithalon is a synthetic tetrapeptide that supports anti-aging, longevity, and overall health. It works by activating telomerase, an enzyme that helps maintain and repair telomeres, which are essential for healthy cell division and extending cellular lifespan.
Anti-Aging & Longevity
• Extends lifespan by 12-17% in studies
• Slows cellular aging processes
• Supports DNA repair mechanisms
Sleep Improvement
• Regulates circadian rhythms
• Improves sleep quality naturally
• Optimizes pineal gland function
Immune System Boost
• Reduces cancer incidence by 25-30%
• Strengthens overall resilience
• Supports healthy aging
How Much to Take
Standard Dosing
Frequency: Once or twice daily
Method: Subcutaneous injection
Timing: Same times each day
Cycle Protocol
Break: 4-6 months between cycles
Frequency: 2-3 cycles per year
Purpose: Anti-aging maintenance
Injection Frequency
• Twice daily (morning & evening)
• Consistent timing preferred
Best Timing
• Evening for sleep enhancement
• Align with circadian rhythm
Dose Adjustment
• Increase to 10 mg if needed
• Monitor response carefully
How Long to Use It
Cyclic Usage Protocol
Epithalon works best with cyclic dosing patterns that mimic natural hormone rhythms. Use for short periods with extended breaks to maintain effectiveness.
Rest Period: 4-6 months between cycles
Annual Frequency: 2-3 cycles per year maximum
Long-term: Years of cyclic use for anti-aging benefits
Important Timing Guidelines
Don't use continuously. Epithalon requires rest periods to maintain effectiveness and prevent tolerance. More is not better with this peptide.
Epithalon is for research purposes only. Effects are cumulative over multiple cycles. Always follow the cyclic protocol and consult healthcare providers before use.
Need-to-Know Information
How It Works
Epithalon activates telomerase, the enzyme that maintains telomeres - the protective caps on your DNA. It also enhances pineal gland function to boost melatonin production and regulate your internal clock.
Think of it as a cellular rejuvenation specialist that helps your cells divide longer by protecting their DNA tips, while also tuning up your internal clock and stress management system. It's like having a microscopic fountain of youth working on multiple aging systems at once.
Key Benefits
• Slows biological aging process
• Extends cellular lifespan
• Protects DNA integrity
• Restores melatonin production
• Improves sleep quality
• Regulates circadian rhythms
• Strengthens immunity
• Enhances overall resilience
• Supports healthy aging
• Supports cellular health
• Better energy levels
• Overall wellness boost
Who Should Avoid It
• Those with uncontrolled health conditions
• People under 25 years old
• Without healthcare consultation
Possible Side Effects
• Temporary headaches or dizziness
• Rare allergic reactions
• Generally very well tolerated
Storage & Injection
Mixing Instructions
• Add 1 mL water to 10 mg vial (10 mg/mL)
• For 5 mg dose, draw 0.5 mL
• Mix gently, don't shake vigorously
Storage Requirements
• Unmixed vials: cool, dry place away from light
• Protect from heat and moisture
• Store at 2-8°C after reconstitution
Injection Sites
• Stomach area (preferred)
• Thigh or upper arm
• Fatty tissue areas
Injection Method
• Insert needle at angle
• Inject slowly and steadily
• Remove needle quickly
Important Tips
• Use sterile technique
• Avoid irritated areas
• Clean site before injection
Example Schedule
Daily Dosing Schedule
Once Daily (Beginner)
Best for: First-time users, general anti-aging
Twice Daily (Advanced)
Evening: 5 mg injection before bed
Best for: Enhanced sleep and anti-aging
Missed Dose Protocol
If you miss a dose, take it as soon as you remember within 4-6 hours. If it's close to your next dose time, skip the missed dose and continue your regular schedule.
Never double dose to make up for a missed injection. Epithalon works best with consistent timing, but occasional missed doses won't significantly impact results.
Extra Tips for Best Results
Track Your Progress
• Monitor energy levels daily
• Note overall health changes
• Track during and after cycles
Healthy Lifestyle
• Exercise regularly
• Manage stress effectively
• Get adequate sleep
Stay Consistent
• Don't skip rest periods
• Maintain regular injection times
• Be patient with results
Hydration & Rest
• Prioritize quality sleep
• Support natural recovery
• Avoid excessive stress
What to Expect: Results Timeline
• Improved sleep quality
• Better energy levels
• Enhanced mood
• Deeper, more restful sleep
• Increased daytime alertness
• Stress resilience improvement
• Cumulative anti-aging benefits
• Long-term cellular health
• Enhanced longevity markers
Important Safety Information
Epithalon is for research purposes only. More is not better - avoid continuous use and always follow the cyclic protocol. Results are cumulative over multiple cycles.
Epithalon is not FDA approved for human therapeutic use. Always consult healthcare providers before use, especially if you have medical conditions or take other medications. Follow institutional safety protocols.
Medical Disclaimer
Epithalon is an investigational research compound not approved by the FDA for human therapeutic use. This information is for educational purposes only and should not be construed as medical advice. Long-term safety data in humans is extremely limited.