Peptide Profile

PEG-MGF

A supercharged version of your muscle's natural repair signal—PEGylated Mechano Growth Factor stays active for days instead of minutes, waking up dormant stem cells to rebuild and strengthen damaged muscle tissue.

Healing & Recovery

Dose Range

100-200mcg

Frequency

Twice weekly

Route

Subcutaneous injection

Cycle Length

4-6 weeks

Onset

Moderate (1-2 weeks)

Evidence

Strong

Compound Profile

Scientific & Efficacy Data

C121H200N42O39 (peptide core)

Molecular Formula

2867.2 g/mol (peptide) + PEG moiety

Molecular Weight

48-72 hours (vs. minutes for unmodified MGF)

Half-Life

High when injected subcutaneously or intramuscularly; PEGylation protects from rapid degradation

Bioavailability

108174-48-7

CAS #

178101625

PubChem ID ↗

Developed By · 2005

Based on Dr. Geoffrey Goldspink's MGF research

University College London (original MGF); PEGylated version developed commercially

Primary Benefits

Muscle Recovery

Activates satellite cells and accelerates muscle repair after training or injury—the most validated effect of MGF peptides

Stem Cell Activation

Specifically triggers quiescent muscle stem cells to proliferate and contribute to tissue repair and growth

Tissue Healing

Shows promise beyond muscle—research demonstrates benefits for bone healing and cardioprotection in preclinical studies

Amino Acid Sequence

PEG-Tyr-Gln-Pro-Pro-Ser-Thr-Asn-Lys-Asn-Thr-Lys-Ser-Gln-Arg-Arg-Lys-Gly-Ser-Thr-Phe-Glu-Glu-Arg-Lys

Dosing

How much
do I take?

Starting Dose

100-150 mcg

Frequency

Twice weekly

Duration

4 weeks

Start low to assess your tolerance. Inject into the muscle group you want to target, ideally after training that muscle. The PEGylation means you don't need daily injections—twice weekly is plenty.

Standard Dose

150-200 mcg

Frequency

Twice weekly

Duration

4-6 weeks

The most commonly used protocol. Many users inject bilaterally into the target muscle after training. Some split the dose between muscle groups worked that day. Take at least 4 weeks off between cycles.

Advanced Dose

200-400 mcg

Frequency

2-3 times weekly

Duration

6-8 weeks

Higher doses used by experienced users with specific recovery goals. Some protocols use daily injections at lower doses (100-150 mcg). Always cycle off—more is not always better with growth factors.

Timing

Best time to take

Inject immediately after training the target muscle group. MGF is naturally released in response to muscle damage/stress, so post-workout timing mimics your body's natural repair process.

With food?

PEG-MGF can be taken regardless of food timing. However, avoid injecting within 30-60 minutes of eating a large meal if injecting intramuscularly, as blood flow changes may affect local distribution.

If stacking

If combining with IGF-1 LR3, use PEG-MGF immediately post-workout and IGF-1 LR3 on rest days or several hours later—they work through different but complementary pathways. Some protocols alternate them.

Adjusting Your Dose

Increase if

  • +You've completed 2+ cycles at starting doses with good tolerance and want enhanced recovery
  • +Your injury or recovery demands require more aggressive support
  • +You're following a high-volume training program with significant muscle breakdown

Decrease if

  • -You experience persistent injection site reactions beyond 48 hours
  • -You notice any blood sugar irregularities
  • -Headaches or fatigue don't resolve within the first week
  • -Any unexpected or concerning side effects develop

Signs of right dose

  • Noticeably faster recovery between training sessions
  • Reduced muscle soreness after intense workouts
  • Injured areas healing more quickly than expected
  • No injection site issues beyond initial mild redness

Dosing Calculator

Calculate Your Exact Dose

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Peptide vial
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mg

Look for a number followed by 'mg' on the vial label (e.g., 5mg, 10mg)

Suitability

Is this
right for me?

Best For

Athletes Recovering from Muscle Injuries

If you've pulled, strained, or torn a muscle, PEG-MGF could be a game-changer. It activates the satellite cells (muscle stem cells) that your body uses to repair damaged fibers, potentially speeding up your return to training.

Serious Lifters and Bodybuilders

Heavy training creates microdamage in your muscles—that's actually how they grow. PEG-MGF amplifies your body's repair response, potentially helping you recover faster between sessions and make more gains from each workout.

Older Adults Concerned About Muscle Loss

As we age, our muscles become less responsive to the 'repair and grow' signals. Research shows older adults don't produce as much MGF after exercise. Supplementing with PEG-MGF might help restore some of that youthful repair capacity.

Post-Surgical Recovery (Under Medical Supervision)

If you're recovering from surgery involving muscle tissue, PEG-MGF's ability to recruit stem cells and promote tissue repair could support faster healing. Always use under medical guidance in these situations.

Consider Alternatives If

Goal: General muscle repair and healing

Consider: BPC-157, TB-500, IGF-1 LR3

Goal: Growth hormone pathway support

Consider: CJC-1295/Ipamorelin, Sermorelin, Tesamorelin

Goal: Bone and connective tissue healing

Consider: BPC-157, TB-500, Pentosan Polysulfate

Who Should Avoid

Do not use if

  • ×You have active cancer or a history of cancer—growth factors can potentially stimulate tumor growth
  • ×You are pregnant or might become pregnant—effects on fetal development are unknown
  • ×You are breastfeeding—the peptide could potentially pass into breast milk
  • ×You have a known allergy to polyethylene glycol (PEG) or peptide components
  • ×You're under 18—PEG-MGF is for adult use only
  • ×You have uncontrolled diabetes—IGF-related compounds can affect blood sugar

Use with caution if

  • !You have controlled diabetes—monitor blood sugar closely as IGF-related peptides can affect glucose
  • !You take insulin or other diabetes medications—risk of hypoglycemia interactions
  • !You have a history of benign tumors or growths—growth factors warrant extra caution
  • !You're on blood thinners—may increase bruising at injection sites
  • !You have autoimmune conditions—immune system effects not fully characterized
  • !You're using other growth factors or peptides—consult a professional about interactions

Not Sure?

Compare PEG-MGF with similar peptides to find the best fit for your goals.

PEG-MGF vs TB-500View all Healing & Recovery peptides

Administration

How do I
use it?

Reconstitution

What you need

  • Bacteriostatic water (BAC water)—the preservative keeps it safe for multiple uses
  • Insulin syringes (29-31 gauge)—thin needles mean less discomfort
  • Alcohol swabs for cleaning vial tops and injection sites
  • Your PEG-MGF powder vial
  • A clean, well-lit workspace

Example

If you have a 2mg (2000 mcg) vial and add 2mL of BAC water, you get a concentration of 1000 mcg/mL. So every 0.1mL (10 units on an insulin syringe) equals 100 mcg of PEG-MGF.

For a 200 mcg dose at 1000 mcg/mL concentration: draw 0.2mL (20 units on a standard insulin syringe). Always double-check your math before injecting.

Injection

Route

Subcutaneous or intramuscular injection—many users prefer intramuscular directly into the target muscle for localized effects

Best sites

  • The specific muscle group you just trained (for targeted recovery)
  • Belly fat area (about 2 inches from belly button) for subcutaneous
  • Front or outer thigh for subcutaneous
  • Deltoid (shoulder) muscle for intramuscular

Technique

  • 1.Wash your hands thoroughly with soap and water
  • 2.Clean the injection site with an alcohol swab and let it air dry
  • 3.For subcutaneous: pinch about an inch of skin and insert at 45-degree angle
  • 4.For intramuscular: insert needle straight (90 degrees) into the muscle belly
  • 5.Push the plunger slowly and steadily—don't rush
  • 6.Wait 5-10 seconds before removing the needle
  • 7.Apply light pressure with a clean swab if needed—don't rub vigorously

Storage

Before reconstitution

Keep your PEG-MGF powder refrigerated (36-46°F / 2-8°C) for short-term storage or frozen (-4°F / -20°C) for long-term storage. Protect from light and moisture. Properly stored powder remains stable for 2+ years when frozen, 6+ months refrigerated.

After reconstitution

Once mixed with bacteriostatic water, refrigerate at 36-46°F (2-8°C). Never freeze reconstituted peptide—freezing destroys it. Keep away from light and use within 3-4 weeks for best potency.

Signs of degradation

  • Cloudy or hazy appearance (should be crystal clear)
  • Visible particles floating or settled at the bottom
  • Color changes—any yellowing or discoloration means toss it
  • Unusual smell—fresh solution should have minimal odor

Sample Daily Schedule

Immediately post-workout

100-200 mcg injection

Site: Into the muscle group trained, or subcutaneous

Inject within 30 minutes of finishing your workout for best timing with natural MGF release. For bilateral muscles (legs, arms), can split dose between both sides.

Second weekly injection (48-72 hours after first)

100-200 mcg injection

Site: Same or different target muscle

Maintain consistent twice-weekly schedule. If not training that day, subcutaneous injection is fine. The extended half-life means you don't need daily dosing.

Safety

Is it
safe?

Safety Profile

PEG-MGF has shown a favorable safety profile in preclinical research, with the pegylation process making it more stable and longer-lasting than unmodified MGF. The E-peptide portion of MGF has been studied for decades and appears well-tolerated. Most reported side effects are mild and injection-site related. However, human clinical trials are limited, and long-term safety data in humans is not yet established.

Most safety data comes from animal studies and the broader body of IGF-1 research. The original MGF (without PEG) was discovered and characterized by Dr. Geoffrey Goldspink's team at University College London. While preclinical results are promising, PEG-MGF remains a research compound without FDA approval. Always work with a knowledgeable healthcare provider.

Common Side Effects

Experienced by some users

Injection site irritation

A small area of redness, mild swelling, or tenderness where you injected is normal. This is your body's response to the injection and solution.

Management: Rotate injection sites between different areas. A cool compress can help. Usually resolves within 24-48 hours.

Mild headache

Some users report mild headaches in the first few days of use, possibly related to the growth factor activity.

Management: Stay well-hydrated and rest if needed. These typically resolve within the first week as your body adjusts.

Temporary fatigue

Feeling a bit tired after injection is normal, especially during the first few uses. Your body is responding to the peptide.

Management: Many users inject post-workout when they'd be resting anyway. Fatigue usually improves after the first few injections.

Less Common

  • Localized swelling
  • Blood sugar fluctuations

These typically resolve with continued use or dose adjustment.

Stop and Seek Help If

  • ×Any signs of allergic reaction—stop immediately and seek medical help
  • ×Injection site reactions that spread, worsen, or don't heal within 72 hours
  • ×Persistent headaches or fatigue that don't improve after the first week
  • ×Any blood sugar problems, especially if diabetic
  • ×Unusual lumps, growths, or tissue changes anywhere in your body
  • ×Your cycle is complete—always take breaks between cycles

PEG-MGF is a research compound, not an FDA-approved medication. Never start, stop, or change your dosing without guidance from a qualified healthcare provider. This information is for educational purposes only—not medical advice. Individual responses can vary significantly.

Interactions

With other peptides

  • Complementary but use different timing—PEG-MGF post-workout activates satellite cells, IGF-1 LR3 on rest days supports protein synthesis. Powerful combination when used correctly.
  • Excellent pairing for comprehensive healing. BPC-157 works on gut and systemic repair while PEG-MGF targets muscle-specific regeneration. Different mechanisms, synergistic effects.
  • Both promote healing but through different pathways. TB-500 is systemic and affects blood vessel formation, PEG-MGF is more muscle-specific. Can be used together.
  • Redundant—PEG-MGF is just a longer-acting version of MGF. Using both wastes money and doesn't add benefit. Choose one or the other.
  • Can be stacked for comprehensive growth hormone axis support. Different targets and mechanisms. Many users combine these successfully.

With medications

  • !Insulin - Potential for additive hypoglycemic effects since IGF-related peptides can lower blood sugar. Use extreme caution and monitor glucose closely.
  • !Diabetes medications (metformin, sulfonylureas) - May interact with glucose regulation. Monitor blood sugar more frequently. Consult your endocrinologist.
  • Blood thinners (warfarin, aspirin) - May increase bruising at injection sites. Not dangerous but monitor for excessive bruising. Inform your doctor.
  • Corticosteroids - Steroids can inhibit muscle repair processes. The interaction may reduce PEG-MGF effectiveness, though both can still be used if medically necessary.

With supplements

  • Creatine - Safe and potentially synergistic—creatine supports muscle energy while PEG-MGF supports repair. Common combination for athletes.
  • Protein supplements - Essential pairing—you need adequate protein for the repair processes PEG-MGF initiates. Make sure you're getting enough.
  • BCAAs - Safe to combine. BCAAs provide building blocks while PEG-MGF signals repair. Complementary mechanisms.
  • High-dose vitamin E - Very high doses of vitamin E may thin blood and increase injection site bruising. Normal supplement doses are fine.

Effectiveness

Does it
work?

Evidence Level

Strong preclinical

What this means

When you exercise hard or injure a muscle, your body produces a special signal called Mechano Growth Factor (MGF). Think of MGF as an alarm that wakes up sleeping stem cells in your muscles called satellite cells. These satellite cells then multiply and fuse with your damaged muscle fibers to repair them and make them stronger. The problem is, natural MGF only lasts a few minutes before your body breaks it down. PEG-MGF solves this by attaching a protective coating (polyethylene glycol) that keeps it active for 2-3 days instead of minutes. This gives your repair cells much more time to respond to the 'wake up' signal.

What to Expect

Week 1

What you might notice

  • Mild injection site redness or soreness (completely normal)
  • Possibly some fatigue or mild headache initially
  • Maybe nothing dramatic yet—the peptide is getting to work at the cellular level
  • Your body is beginning to respond to the satellite cell activation signal

What's normal

  • Minor injection site reactions that fade within a day or two
  • No dramatic visible changes yet
  • Feeling normal aside from minor injection-related effects

What's next

  • Maintain your twice-weekly schedule consistently
  • Track injection sites and any effects in a simple log
  • Continue your normal training—PEG-MGF works best with exercise stimulus

Weeks 2-3

What you might notice

  • Potentially faster recovery between training sessions
  • Reduced muscle soreness (DOMS) after hard workouts
  • Injured areas may start feeling better faster than usual
  • Injection site reactions typically decrease as technique improves

What's normal

  • Subtle improvements in recovery times
  • Feeling like you can train hard more frequently
  • Injured muscles feeling progressively better

What's next

  • Continue consistent protocol
  • Push your training appropriately—the peptide supports recovery
  • Note any changes in how you feel between sessions

Weeks 4-6

What you might notice

  • Clear improvements in recovery capacity
  • Muscle injuries showing meaningful healing progress
  • Potentially some improvements in muscle fullness/quality
  • Established routine with minimal side effects

What's normal

  • Feeling stronger in your recovery abilities
  • Being able to handle higher training volumes
  • Injured areas continuing to improve

What's next

  • Complete your cycle (don't exceed 8 weeks without a break)
  • Plan for at least 4 weeks off before your next cycle
  • Maintain good training and nutrition during the break

Signs It's Working

Recovery Indicators

  • Less muscle soreness 24-48 hours after hard training
  • Able to train the same muscle group again sooner
  • Feeling fresh rather than beat up between sessions
  • Injured muscles healing faster than your normal experience

Performance Indicators

  • Maintaining or increasing strength during high-volume phases
  • Better tolerance for increased training frequency
  • Consistent performance without accumulating fatigue
  • Injured areas returning to full function more quickly

Physical Indicators

  • Potentially improved muscle fullness and quality over time
  • Reduced visible bruising or damage after intense training
  • Better overall sense of physical resilience
  • Injured sites showing visible healing progress

Not Seeing Results?

Common reasons

  • Not training hard enough—PEG-MGF responds to muscle damage/stress, so light workouts won't give it much to work with
  • Poor injection timing—injecting long after training misses the optimal window when your body expects the MGF signal
  • Inadequate protein intake—PEG-MGF signals repair but your body needs building materials (amino acids) to actually rebuild
  • Expecting steroid-like gains—PEG-MGF enhances recovery, not anabolic capacity; results are more subtle than steroids
  • Degraded product from poor storage or unreliable source—always get third-party testing certificates (COAs)
  • Inconsistent dosing schedule—skipping doses or irregular timing reduces effectiveness

Key Research

"Mechano Growth Factor E peptide (MGF-E), derived from an isoform of IGF-1, activates human muscle progenitor cells and induces an increase in their fusion potential at different ages"

Kandalla PK, Goldspink G, Butler-Browne G, Mouly V, 2011

Finding: This landmark study showed that the MGF E-peptide significantly increases the proliferative lifespan of satellite cells from young adults and delays their senescence. It boosted the cells' ability to fuse and repair muscle—exactly what you want for recovery. Importantly, researchers noted MGF could provide a new strategy against age-related muscle loss without the cancer risks of full IGF-1.

View Study

"Expression and splicing of the insulin-like growth factor gene in rodent muscle is associated with muscle satellite (stem) cell activation following local tissue damage"

Hill M, Goldspink G, 2003

Finding: This foundational study from the lab that discovered MGF showed that after muscle damage, MGF is rapidly produced first and triggers satellite cell activation. Then the systemic IGF-1 form takes over to complete repair. This established the crucial 'first responder' role of MGF in muscle healing.

View Study

"Sustained delivery of MGF peptide from microrods attracts stem cells and reduces apoptosis of myocytes"

Doroudian G, Pinney J, Ayala P, et al., 2014

Finding: Researchers showed that MGF peptide not only attracts stem cells to damaged areas but also protects heart muscle cells from dying under low-oxygen conditions. The MGF treatment increased stem cell migration and boosted the survival protein Bcl-2—demonstrating both the homing and protective effects of this peptide.

View Study

"Mechano growth factor E peptide promotes osteoblasts proliferation and bone-defect healing in rabbits"

Deng M, Zhang B, Wang K, et al., 2011

Finding: In a study that went beyond just muscle, researchers found that MGF E-peptide was actually more effective at stimulating bone-building cells than full IGF-1. Rabbits with bone defects healed significantly faster when treated with MGF. This opens doors for MGF in fracture healing and bone repair applications.

View Study

"Expression of IGF-I splice variants in young and old human skeletal muscle after high resistance exercise"

Hameed M, Orrell RW, Cobbold M, Goldspink G, Harridge SD, 2003

Finding: This human study revealed a critical age-related problem: after resistance exercise, young people showed a significant increase in MGF, but elderly subjects did not. This 'MGF deaf' response to exercise in older adults may explain age-related muscle loss—and suggests supplementing MGF could restore some youthful repair capacity.

View Study

Frequently Asked Questions

Medical Disclaimer

PEG-MGF is an investigational research compound not approved by the FDA for human therapeutic use. This information is for educational purposes only and should not be construed as medical advice. Always consult with a qualified healthcare provider before starting any new supplement or treatment protocol.

Last updated: 1/19/2026