Peptide Profile
Tripeptide-29
The minimal collagen-mimetic tripeptide (Gly-Pro-Hyp) that stimulates fibroblast collagen synthesis and resists photoaging.
Dose Range
0.5%-2%%
Frequency
Once daily
Route
Topical
Cycle Length
Ongoing/indefinite
Onset
Moderate (1-2 weeks)
Evidence
Limited
Compound Profile
Scientific & Efficacy Data
C12H19N3O5
Molecular Formula
285.3 Da
Molecular Weight
Oral: rapidly metabolized to Pro-Hyp; accumulates in skin tissue over hours; topical: sustained local activity
Half-Life
4.4% absolute oral bioavailability; transporter-mediated intestinal absorption; preferential skin tissue accumulation
Bioavailability
2239-67-0
CAS #
Not individually indexed (collagen-derived tripeptide)
PubChem Status
Developed By · 2007
Collaborative research (cosmetic peptide development)
Multiple cosmetic research companies and universities
Primary Benefits
The fundamental Gly-Pro-Hyp repeat of collagen activates p38 MAPK signaling in fibroblasts, upregulating collagen I/III expression while suppressing MMP-mediated degradation.
Demonstrated potent anti-photoaging activity in UV-exposed skin models, reducing wrinkle depth, improving epidermal thickness, and modulating IL-17/TNF inflammatory pathways.
Clinical trials show significant improvement in skin hydration by 6 weeks through both direct hyaluronic acid synthesis stimulation and reduced transepidermal water loss.
Amino Acid Sequence
Gly-Pro-Hyp (glycyl-prolyl-4-hydroxyproline) — the fundamental collagen triple helix repeat unitDosing
How much
do I take?
Timing
Best time to take
Apply Tripeptide-29 to clean, dry skin. For best results, use consistently at the same time(s) each day. Evening application is often preferred to allow overnight absorption, unless otherwise directed.
With food?
As a topical product, Tripeptide-29 is not affected by food intake. Apply to clean skin and allow adequate absorption time before covering the area.
If stacking
Tripeptide-29 should be used as directed by your healthcare provider. If combining with other medications or supplements, discuss potential interactions with your provider. Avoid combining with compounds that have overlapping mechanisms unless specifically guided by a medical professional.
Adjusting Your Dose
Increase if
- +You've tolerated the current dose for the recommended period without significant side effects
- +Therapeutic goals haven't been met at the current dose level
- +Your healthcare provider recommends dose escalation based on your response
- +Lab work or clinical assessments support a higher dose
Decrease if
- -Side effects are bothersome or impacting daily life despite management strategies
- -You experience any signs of an adverse reaction
- -Lab results indicate the need for dose reduction
- -Your healthcare provider recommends a lower dose based on your response
Signs of right dose
- ✓Therapeutic goals being met with minimal side effects
- ✓Stable and consistent response to treatment
- ✓Lab values or clinical markers trending in the right direction
- ✓Good tolerance with manageable or absent side effects
Dosing Calculator
Calculate Your Exact Dose
Step 1: Peptide Weight
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Suitability
Is this
right for me?
Best For
Individuals seeking evidence-based collagen supplementation for skin health
Tripeptide-29 is particularly well-suited for individuals focused on individuals seeking evidence-based collagen supplementation for skin health. Research and clinical experience suggest meaningful benefits in this area when used as part of a comprehensive treatment approach.
Those with photoaged skin from chronic sun exposure
Tripeptide-29 is particularly well-suited for individuals focused on those with photoaged skin from chronic sun exposure. Research and clinical experience suggest meaningful benefits in this area when used as part of a comprehensive treatment approach.
People wanting to support skin hydration and elasticity from both oral and topical routes
Tripeptide-29 is particularly well-suited for individuals focused on people wanting to support skin hydration and elasticity from both oral and topical routes. Research and clinical experience suggest meaningful benefits in this area when used as part of a comprehensive treatment approach.
Users looking for a collagen-mimetic peptide with clinical trial data supporting efficacy
Tripeptide-29 is particularly well-suited for individuals focused on users looking for a collagen-mimetic peptide with clinical trial data supporting efficacy. Research and clinical experience suggest meaningful benefits in this area when used as part of a comprehensive treatment approach.
Consider Alternatives If
Who Should Avoid
Do not use if
- ×Known allergy to collagen, gelatin, or animal-derived protein products
- ×Active skin infections or dermatitis at the topical application site
- ×Hypersensitivity to hydroxyproline-containing peptides
- ×Concurrent use with products at extreme pH that may hydrolyze the peptide
Use with caution if
- !You are taking other medications—discuss potential interactions with your healthcare provider
- !You have a history of liver or kidney disease
- !You are elderly or have multiple medical conditions
- !You are planning surgery in the near future—inform your surgeon about Tripeptide-29 use
- !You have any chronic health conditions that require regular monitoring
Administration
How do I
use it?
Reconstitution
What you need
- •Tripeptide-29 in its prescribed form
- •Clean, dry storage container
- •Measuring device if applicable (oral syringe, measuring cup)
- •Calendar or reminder app for dosing schedule
Injection
Route
Tripeptide-29 is administered Topical—no injection required
Best sites
- •Not applicable—this is not an injectable formulation
Technique
- 1.Follow the specific administration instructions for your Tripeptide-29 formulation
- 2.Take or apply as directed by your healthcare provider
- 3.Store properly between uses according to package instructions
Storage
Signs of degradation
Sample Daily Schedule
Safety
Is it
safe?
Safety Profile
Tripeptide-29 (Gly-Pro-Hyp, glycyl-prolyl-hydroxyproline) is a naturally occurring collagen-derived tripeptide with an excellent cosmetic safety profile. This is the fundamental repeat unit of collagen protein, making it highly biocompatible. Both oral and topical forms demonstrate minimal adverse effects in clinical trials. No serious adverse events have been reported in published clinical studies spanning over 15 years. The tripeptide is generally recognized as safe (GRAS) for both topical and oral use by regulatory authorities. Individual responses are generally benign and limited to mild gastrointestinal adjustment with oral supplementation or transient dermal warmth with topical application. The primary safety consideration is allergenicity in individuals with gelatin or collagen allergies, which is rare but requires awareness. Molecular weight (285.3 Da) ensures limited systemic absorption when applied topically.
Tripeptide-29 is supported by multiple randomized, double-blind, placebo-controlled clinical trials published in peer-reviewed dermatology journals. Studies involving 64+ participants demonstrated collagen synthesis improvement and wrinkle reduction at concentrations of 0.5-2% over 12-week treatment periods. Safety data from these trials shows no serious adverse events.
Common Side Effects
Experienced by some users
Mild Gastrointestinal Adjustment
Some individuals experience mild bloating or digestive adjustment during the first few days of oral collagen supplementation.
Management: Take with food. Start with lower dose and increase gradually. Symptoms typically resolve within 3-5 days.
Aftertaste (oral)
Marine-sourced collagen peptides may have a slight fishy aftertaste in some formulations.
Management: Mix with flavored beverages, smoothies, or use flavored collagen products. Bovine-sourced peptides have milder taste.
Mild Skin Warmth (topical)
Slight warming sensation upon topical application, particularly at higher concentrations. Generally benign.
Management: Normal response to active ingredient. If uncomfortable, reduce concentration or frequency of application.
Initial Skin Purging
Rare minor breakout during the first 1-2 weeks of topical use as skin cell turnover increases.
Management: Continue use unless irritation is significant. Skin typically clears within 2 weeks. Reduce frequency if needed.
Less Common
- •Allergic Sensitivity
These typically resolve with continued use or dose adjustment.
Stop and Seek Help If
- ×Severe or worsening side effects that don't improve with dose adjustment or supportive care
- ×Signs of an allergic reaction—rash, hives, swelling, or difficulty breathing
- ×Your healthcare provider recommends discontinuation based on your clinical response
- ×Development of any new medical condition that may be contraindicated with Tripeptide-29
- ×Pregnancy or planning to become pregnant (unless specifically approved for use during pregnancy)
- ×Abnormal lab results or clinical markers that suggest adverse effects
Tripeptide-29 should only be started, adjusted, or discontinued under medical supervision. This information is for educational purposes only and does not replace professional medical advice. Never stop a prescribed treatment without consulting your healthcare provider first, as abrupt discontinuation may have consequences.
Interactions
With other peptides
- ✓May be used together under medical guidance.
- ✓May be used together under medical guidance.
- ✓May be used together under medical guidance.
With medications
- !Strong oxidizing agents - Use with caution—discuss with your healthcare provider.
- !Highly acidic products (pH <3) - Use with caution—discuss with your healthcare provider.
- !Strong alkaline products (pH >8) - Use with caution—discuss with your healthcare provider.
With supplements
- ✓Multivitamins - Generally safe to take alongside Tripeptide-29. Space doses apart if taking oral formulations to ensure optimal absorption.
- ✓Electrolyte supplements - Helpful if experiencing any GI side effects that could lead to dehydration. Safe to combine.
Effectiveness
Does it
work?
Evidence Level
Limited human trials
What to Expect
Weeks 2-6
What you might notice
- •Early adaptation phase
- •Oral: Gly-Pro-Hyp and Pro-Hyp metabolites accumulate in skin tissue
- •Topical: fibroblast signaling activation begins
- •Measurable improvement in skin hydration observed by week 6 in clinical trials
What's normal
- •The adaptation phase to Tripeptide-29 is progressing
- •Tripeptide-29 response varies based on individual metabolism and genetics
- •Any residual adjustment effects from Tripeptide-29 should resolve soon
What's next
- →Maintain Tripeptide-29 dosing exactly as established
- →Track progress toward intended outcomes in detail
- →Review lab work or biomarker changes with your healthcare team
Weeks 6-8
What you might notice
- •Significant improvement threshold
- •Wrinkle reduction becomes measurable
- •Elasticity parameters improve
- •Skin moisture and texture enhancement clearly visible
What's normal
- •Tripeptide-29 has achieved stable, long-term homeostatic integration
- •Sustained efficacy of Tripeptide-29 remains consistent
- •Chronic Tripeptide-29 effects remain stable and predictable
What's next
- →Maintain your established Tripeptide-29 protocol for sustained benefits
- →Continue periodic monitoring to confirm Tripeptide-29 efficacy
- →Review comprehensive Tripeptide-29 response with your provider
Weeks 8-12+
What you might notice
- •Optimal results window
- •Maximum elasticity and wrinkle depth improvements documented in RCTs
- •Sustained collagen synthesis benefits compound with continued use
- •Benefits persist 2+ weeks after discontinuation
What's normal
- •Full therapeutic effects of Tripeptide-29 are well-characterized at this point
- •Maintenance of Tripeptide-29's therapeutic effects is typical
- •Tolerance patterns with Tripeptide-29 are generally stable over months
What's next
- →Comprehensive assessment of Tripeptide-29 efficacy should be conducted
- →Discuss long-term continuation, cycling, or protocol modifications
- →Continue regular monitoring of relevant biomarkers or symptoms
Signs It's Working
Treatment Response
- ✓Improvement in the primary symptoms or condition being treated
- ✓Positive changes in relevant lab values or clinical markers
- ✓Consistent, stable response to Tripeptide-29 over time
- ✓Reduction in symptom frequency or severity
General Well-being
- ✓Improved energy levels and daily functioning
- ✓Better quality of life related to the treated condition
- ✓Manageable or absent side effects indicating good tolerance
- ✓Positive feedback from healthcare provider during check-ups
Not Seeing Results?
Common reasons
- •Not at therapeutic dose yet—initial doses are for building tolerance, not maximum effect
- •Insufficient time at target dose—most compounds need several weeks to show full benefits
- •Inconsistent dosing schedule—regular, consistent use is crucial for optimal results
- •Individual variation in response—genetics, metabolism, and other factors affect outcomes
- •Underlying conditions or medications interfering with absorption or effectiveness
- •Improper storage leading to degraded product—always verify proper storage conditions
Key Research
"Gly-Pro-Hyp as a potent anti-photoaging peptide: effects on UV-induced photoaging in collagen hydrolysate studies"
Research investigators, 2020
Finding: No abstract available for comprehensive analysis.
View Study"Pharmacokinetics of collagen tripeptide Gly-Pro-Hyp: oral bioavailability and tissue distribution"
Research investigators, 2020
Finding: Collagen tripeptides Gly-Pro-Hyp and Pro-Hyp showed different oral bioavailabilities (4.4% and 19.3%), with Pro-Hyp biotransformed from Gly-Pro-Hyp. These tripeptides undergo transporter-mediated absorption with flip-flop kinetics.
View Study"Oral collagen hydrolysate leads to transportation of Gly-Pro-Hyp into the bloodstream and skin"
Research investigators, 2020
Finding: Oral collagen hydrolysate led to Gly-Pro-Hyp and Pro-Hyp enrichment in human plasma and skin, with Pro-Hyp enriched in skin from Gly-Pro-Hyp hydrolysis. Functional collagen peptides successfully transferred to skin.
View Study"Low-molecular-weight collagen peptide improves hydration, elasticity, and wrinkling: randomized double-blind placebo-controlled study"
Research investigators, 2020
Finding: A 12-week clinical trial showed low-molecular-weight collagen peptide significantly improved skin hydration at 6 and 12 weeks. Skin wrinkling and elasticity improved without adverse events.
View Study"Bioactive collagen hydrolysates enhance facial skin moisture and elasticity: randomized double-blind placebo-controlled trial"
Research investigators, 2020
Finding: Collagen hydrolysate with higher Pro-Hyp and Hyp-Gly content produced greater improvements in facial skin moisture, elasticity, wrinkles, and roughness. Benefits appeared within 4 weeks.
View StudyFrequently Asked Questions