Peptide Profile

AOD-9604

A modified fragment of human growth hormone that targets fat cells for breakdown without affecting blood sugar or muscle growth—your metabolism's precision tool.

Weight Management

Dose Range

250-500mcg

Frequency

Once daily

Route

Subcutaneous injection

Cycle Length

8-12 weeks

Onset

Gradual (3-4 weeks)

Evidence

Moderate

Compound Profile

Scientific & Efficacy Data

C78H123N23O23S2

Molecular Formula

1815.1 g/mol

Molecular Weight

~3-4 minutes (plasma)

Half-Life

High (subcutaneous)

Bioavailability

221231-10-3

CAS #

71300630

PubChem ID ↗

Developed By · 1997

Frank Ng

Monash University / Metabolic Pharmaceuticals

Primary Benefits

Fat Targeting

Specifically targets fat cells through beta-3 receptor activation without affecting muscle, blood sugar, or other tissues—one of the most selective fat-targeting compounds available

Safety Profile

Extensive human clinical trial data showing excellent tolerability with no effect on glucose metabolism, insulin levels, or IGF-1—safer than full HGH for metabolic support

Joint Support

Emerging research demonstrates cartilage regeneration properties, with animal studies showing enhanced joint repair especially when combined with hyaluronic acid

Amino Acid Sequence

Tyr-Leu-Arg-Ile-Val-Gln-Cys-Arg-Ser-Val-Glu-Gly-Ser-Cys-Gly-Phe

Dosing

How much
do I take?

Starting Dose

250 mcg

Frequency

Once daily

Duration

2-4 weeks

Begin here to assess tolerance. Inject on an empty stomach, ideally in the morning before food or exercise.

Standard Dose

300-400 mcg

Frequency

Once daily

Duration

8-12 weeks

The most commonly used dose range for consistent fat metabolism support. Best taken fasted in the morning.

Advanced Dose

500 mcg

Frequency

Once daily

Duration

8-12 weeks

Maximum dose used in clinical studies. Some users split into two 250mcg doses (morning and before bed).

Timing

Best time to take

Morning on an empty stomach, 30-60 minutes before food or exercise

With food?

Best taken on an empty stomach for optimal absorption. Wait at least 30 minutes before eating.

If stacking

Can be combined with GHRP peptides like Ipamorelin at the same time. If using with CJC-1295, inject together for synergistic effects on fat metabolism.

Adjusting Your Dose

Increase if

  • +Tolerating current dose well with no side effects after 2-3 weeks
  • +Fat loss progress has stalled despite consistent diet and exercise
  • +Seeking enhanced fat oxidation during intensive training phases

Decrease if

  • -Experiencing persistent headaches or flushing
  • -Injection site reactions are bothersome
  • -Any signs of allergic reaction occur

Signs of right dose

  • Gradual reduction in body fat, especially in stubborn areas
  • No significant side effects
  • Improved body composition without muscle loss
  • Stable energy levels throughout the day

Dosing Calculator

Calculate Your Exact Dose

Peptide in Vial

mg

BAC Water Added

2ml
ml

Desired Dose

mcg

Amount to Draw

10

units

0.0ml0.1ml0.2ml0.3ml0.4ml0.5ml0.6ml0.7ml0.8ml0.9ml1.0ml
0102030405060708090100

Concentration

2.50 mg/ml (2500 mcg/ml)

Suitability

Is this
right for me?

Best For

Targeted Fat Loss

AOD-9604 shines when you want to target stubborn fat deposits. It works by mimicking how growth hormone breaks down fat, but without the side effects of actual HGH. Think of it as a smart missile that targets fat cells specifically.

Body Recomposition

Perfect for those who want to lose fat while maintaining muscle. Unlike many fat loss compounds, AOD-9604 doesn't affect muscle tissue or mess with your blood sugar levels—it's laser-focused on fat metabolism.

Metabolic Enhancement

Ideal for boosting your metabolism naturally. AOD-9604 increases fat oxidation (your body's ability to burn fat for fuel) without the jittery stimulant effects of caffeine or other thermogenics.

Joint Health Support

Emerging research shows AOD-9604 may help repair cartilage and support joint health. Studies in osteoarthritis models show it can reduce cartilage damage, making it a dual-purpose peptide for active individuals.

Metabolically Safe Fat Loss

Unlike many weight loss compounds, AOD-9604 doesn't raise blood sugar, doesn't affect insulin, and doesn't stimulate cell growth. It's one of the gentlest approaches to enhancing fat metabolism available.

Consider Alternatives If

Goal: More potent fat loss with appetite suppression

Consider: Semaglutide, Tirzepatide, Tesofensine

Goal: Growth hormone benefits beyond fat loss

Consider: CJC-1295/Ipamorelin, Tesamorelin, Sermorelin

Goal: Non-peptide metabolic support

Consider: 5-Amino-1MQ, MOTS-c, Berberine

Who Should Avoid

Do not use if

  • ×Pregnant or breastfeeding—safety not established
  • ×Active cancer or history of cancer—any growth-related peptide should be avoided
  • ×Under 18 years of age—not studied in children or adolescents
  • ×Known allergy to AOD-9604 or growth hormone peptides
  • ×Severe liver or kidney disease—clearance may be affected

Use with caution if

  • !Taking blood thinners—monitor for any unusual bruising
  • !History of autoimmune conditions—limited data available
  • !Currently using other growth hormone-related compounds
  • !History of blood clotting disorders
  • !Taking diabetes medications—though AOD-9604 shouldn't affect blood sugar, monitor closely
  • !Planning surgery in the near future—discuss with your surgeon

Not Sure?

Compare AOD-9604 with similar peptides to find the best fit for your goals.

Administration

How do I
use it?

Reconstitution

What you need

  • Bacteriostatic water (BAC water)
  • Insulin syringe (29-31 gauge)
  • Alcohol swabs
  • Sterile AOD-9604 vial (typically 5mg)

Example

5mg vial + 2mL BAC water = 2.5mg/mL (2500mcg/mL). For 250mcg dose, draw 0.1mL (10 units on insulin syringe).

At 2500mcg/mL concentration: 0.1mL = 250mcg, 0.15mL = 375mcg, 0.2mL = 500mcg

Injection

Route

Subcutaneous injection into fatty tissue

Best sites

  • Abdomen (2 inches from navel)—most common site for fat-targeting
  • Love handle area—some prefer injecting near target fat areas
  • Outer thigh (middle third)

Technique

  • 1.Clean injection site thoroughly with alcohol swab and let dry
  • 2.Pinch a fold of skin between thumb and forefinger
  • 3.Insert needle at 45-90 degree angle into the pinched fold
  • 4.Inject slowly and steadily
  • 5.Remove needle and apply light pressure—do not rub

Storage

Before reconstitution

Refrigerate at 36-46°F (2-8°C) for up to several months. Can also be stored frozen at -4°F (-20°C) for longer-term storage. Keep in original packaging away from light.

After reconstitution

Refrigerate immediately at 36-46°F (2-8°C). Never freeze after mixing with bacteriostatic water. Store away from light. Discard after 28 days or if any degradation signs appear.

Signs of degradation

  • Cloudy or milky appearance (should be clear)
  • Visible particles or floaters in solution
  • Unusual color change
  • Clumping or precipitation at bottom of vial

Safety

Is it
safe?

Safety Profile

AOD-9604 has been studied in multiple human clinical trials involving thousands of participants. The safety profile is notably clean—it doesn't affect blood sugar, insulin levels, or cause the typical side effects of growth hormone. Phase 2 trials showed it was well-tolerated even at doses higher than typically used. The most common side effects are mild and injection-site related.

Metabolic Pharmaceuticals invested over $50 million in research, generating extensive human safety data. Multiple Phase 1-2 trials demonstrated excellent tolerability. The compound received GRAS (Generally Recognized as Safe) status for oral use in some jurisdictions. However, Phase 3 trials for obesity were not completed.

Common Side Effects

Experienced by some users

Injection site reactions

Redness, minor swelling, or tenderness at the injection site that typically resolves within hours

Management: Rotate injection sites, ensure proper technique, apply ice if needed

Mild headache

Occasional headache, especially in the first few days of use

Management: Stay well hydrated, usually resolves with continued use

Flushing or warmth

Temporary flushing sensation after injection, lasting minutes

Management: This is normal and harmless—resolves quickly on its own

Less Common

  • Fatigue
  • Dizziness

These typically resolve with continued use or dose adjustment.

Stop and Seek Help If

  • ×Signs of allergic reaction (rash, swelling, difficulty breathing)
  • ×Persistent side effects that don't improve after dose reduction
  • ×Pregnancy confirmed or suspected
  • ×Development of any new medical condition—consult your provider
  • ×No noticeable benefits after 8-12 weeks of consistent use
  • ×Prior to any surgical procedure—discuss timing with your surgeon

Always consult with a healthcare provider before stopping or if you experience any concerning symptoms. This information is for educational purposes and should not replace professional medical advice.

Interactions

With other peptides

  • Excellent synergy for fat loss—can be injected together
  • Commonly stacked for body recomposition—complementary mechanisms
  • Safe to combine; different mechanisms, no interaction concerns
  • Safe combination for those seeking fat loss plus recovery benefits
  • !Generally not combined—AOD-9604 mimics part of HGH's action

With medications

  • Insulin - AOD-9604 does not affect blood sugar, but monitor if diabetic
  • Diabetes medications - No known interactions, but monitor blood sugar as always
  • Blood thinners - Use caution—may slightly increase bruising at injection sites
  • Thyroid medications - No known interactions, safe to use together

With supplements

  • L-Carnitine - May enhance fat oxidation effects—good synergy
  • Caffeine/Thermogenics - Safe to combine for enhanced fat loss, but not necessary
  • Fish oil/Omega-3s - Safe and may support overall metabolic health
  • Protein supplements - Recommended to support muscle preservation during fat loss

Effectiveness

Does it
work?

Evidence Level

Moderate human trials

What this means

AOD-9604 is like a tiny piece of growth hormone that only does one job: tell your fat cells to release their stored fat. Your body naturally uses growth hormone to burn fat, but full HGH comes with side effects like blood sugar problems and unwanted growth. Scientists figured out which part of growth hormone handles fat-burning and made AOD-9604 from just that piece. So you get the fat-burning signal without any of the other effects—your blood sugar stays normal, and you don't grow bigger hands or feet!

What to Expect

Week 1-2

What you might notice

  • Getting used to the injection routine
  • Possibly mild injection site reactions (normal)
  • No dramatic changes yet—this is expected
  • Perhaps subtle increase in energy during fasted morning

What's normal

  • Not seeing visible fat loss yet
  • Minor redness at injection sites
  • Feeling exactly the same as before

What's next

  • Continue consistent daily dosing
  • Maintain your diet and exercise routine
  • Track your starting measurements and photos

Week 3-4

What you might notice

  • Subtle changes in how clothes fit
  • Possibly noticing slightly more definition
  • Body may be responding to enhanced fat oxidation
  • Energy levels during fasted cardio may improve

What's normal

  • Changes are gradual—not dramatic transformations
  • Scale weight may not change much (fat loss, water shifts)
  • Some days feel more effective than others

What's next

  • Consider increasing dose to 300-400mcg if tolerating well
  • Continue tracking measurements
  • Stay consistent with timing and fasted administration

Week 5-8

What you might notice

  • More noticeable fat loss, especially in stubborn areas
  • Improved body composition visible in mirror
  • Clothes fitting noticeably looser in problem areas
  • Enhanced results from your workout program

What's normal

  • Fat loss is gradual—typically 0.5-1 lb fat per week
  • Results vary based on diet and exercise consistency
  • Some areas respond faster than others

What's next

  • Continue at current dose if seeing progress
  • May increase to 500mcg if needed and tolerating well
  • Reassess at week 8 to decide on continuation

Week 9-12+

What you might notice

  • Significant improvement in body composition
  • Stubborn fat areas showing real reduction
  • Potentially 3-6+ pounds of fat lost (with proper diet)
  • Better muscle definition as fat decreases

What's normal

  • Rate of fat loss may slow as you get leaner
  • Results plateau if diet isn't optimized
  • Some users continue beyond 12 weeks

What's next

  • Evaluate results and decide on continuing or taking a break
  • Some users cycle off for 4-6 weeks before resuming
  • Maintain results with diet and exercise during breaks

Signs It's Working

Fat Loss Progress

  • Gradual reduction in body fat percentage
  • Clothes fitting looser, especially around midsection
  • Visible improvement in stubborn fat areas
  • Better muscle definition becoming apparent
  • Progress photos showing real differences over time

Metabolic Response

  • Improved energy during fasted exercise sessions
  • Body responding better to your fat loss diet
  • Enhanced fat oxidation during cardio (feeling warmer)
  • Maintaining muscle while losing fat
  • Stable energy levels throughout the day

Joint Health (if applicable)

  • Reduced joint discomfort during exercise
  • Improved mobility and flexibility
  • Less stiffness after workouts
  • Better recovery from joint stress

Not Seeing Results?

Common reasons

  • Not taking on an empty stomach—fat and food reduce effectiveness
  • Inconsistent dosing—daily consistency is important
  • Dose too low—may need to increase to 400-500mcg
  • Diet not optimized—AOD-9604 enhances fat loss but doesn't replace caloric deficit
  • Not enough time—real results take 6-8+ weeks of consistent use
  • Poor peptide quality—ensure you're getting from a reputable source
  • Expectations too high—AOD-9604 is a gentle compound, not a dramatic fat burner

Key Research

"Metabolic studies of a synthetic lipolytic domain (AOD9604) of human growth hormone"

Ng FM et al., 2000

Finding: AOD-9604 reduced body weight gain by over 50% in obese rats without affecting insulin sensitivity—showing it targets fat metabolism safely without diabetic side effects.

View Study

"Increase of fat oxidation and weight loss in obese mice caused by chronic treatment with human growth hormone or a modified C-terminal fragment"

Heffernan MA et al., 2001

Finding: Both growth hormone and AOD-9604 increased fat oxidation and reduced weight gain, but unlike HGH, AOD-9604 did not cause high blood sugar or reduce insulin—making it a safer option.

View Study

"The effects of human GH and its lipolytic fragment (AOD9604) on lipid metabolism following chronic treatment in obese mice and beta(3)-AR knock-out mice"

Heffernan M et al., 2001

Finding: AOD-9604 works through beta-3 adrenergic receptors on fat cells—it increases expression of these fat-burning receptors, explaining its targeted fat loss mechanism.

View Study

"Effect of Intra-articular Injection of AOD9604 with or without Hyaluronic Acid in Rabbit Osteoarthritis Model"

Kwon DR et al., 2015

Finding: AOD-9604 injections enhanced cartilage regeneration in arthritic joints, with the combination of AOD-9604 plus hyaluronic acid showing the best results for joint repair.

View Study

Frequently Asked Questions

Medical Disclaimer

AOD-9604 is an investigational research compound not approved by the FDA for human therapeutic use. This information is for educational purposes only and should not be construed as medical advice. Always consult with a qualified healthcare provider before starting any new supplement or treatment protocol.

Last updated: 1/15/2026