Peptide Profile

Human Chorionic Gonadotropin (HCG)

A powerful hormone that mimics luteinizing hormone (LH) to stimulate your body's natural testosterone production while keeping your fertility intact—like having your cake and eating it too for men on hormone therapy.

Hormone Support

Dose Range

250-2000IU

Frequency

Twice weekly

Route

Subcutaneous injection

Cycle Length

Ongoing/indefinite

Onset

Moderate (1-2 weeks)

Evidence

Strong

Compound Profile

Scientific & Efficacy Data

C1105H1770N318O336S26 (approximate)

Molecular Formula

36,700 g/mol (approximately)

Molecular Weight

24-36 hours

Half-Life

High when injected subcutaneously or intramuscularly

Bioavailability

9002-61-3

CAS #

Not assigned (complex glycoprotein)

PubChem ID ↗

Developed By · 1920

Bernhard Zondek and Selmar Aschheim

Charité University Hospital, Berlin

Primary Benefits

Fertility Preservation

Gold standard for maintaining sperm production during TRT—research shows ~80% success rate in preserving or restoring spermatogenesis

Testicular Health

Highly effective at preventing and reversing testicular atrophy caused by external testosterone or steroid use

Hormone Balance

Supports natural testosterone production and helps maintain healthy intratesticular hormone levels alongside TRT

Amino Acid Sequence

Heterodimeric glycoprotein: alpha subunit (92 amino acids) common to LH, FSH, TSH; beta subunit (145 amino acids) unique to HCG

Dosing

How much
do I take?

Starting Dose

250-500 IU

Frequency

Twice weekly

Duration

Ongoing with TRT

This is a conservative starting point for men using HCG alongside testosterone therapy. The goal is to maintain testicular function and fertility without overstimulating estrogen production. Start low and adjust based on lab work and how you feel.

Standard Dose

500-1000 IU

Frequency

Twice weekly

Duration

Ongoing with TRT or standalone

The most commonly prescribed range for men on TRT who want to maintain testicular size and fertility. Many clinics use 500 IU two to three times per week as their standard protocol. Monitor estrogen levels as HCG can increase aromatization.

Advanced Dose

1000-2000 IU

Frequency

Two to three times weekly

Duration

12-16 weeks for restart protocols

Higher doses are typically used for fertility induction in hypogonadotropic hypogonadism or as part of post-cycle therapy after anabolic steroid use. These protocols often include FSH as well. Requires careful medical supervision and monitoring of estrogen and testosterone levels.

Timing

Best time to take

Most men inject HCG on set days—for example, Monday and Thursday, or Sunday and Wednesday. Time of day doesn't matter much, but consistency helps you remember. Some prefer morning injections.

With food?

HCG can be taken regardless of food timing. Absorption is not affected by meals since it's injected, not taken orally.

If stacking

When using with testosterone, inject HCG on the same days or alternate days—both approaches work. If using an aromatase inhibitor, take it as prescribed to manage any estrogen increase from HCG.

Adjusting Your Dose

Increase if

  • +Testicular volume continues to decrease despite current dose
  • +Sperm count remains suppressed when fertility is the goal
  • +Your doctor determines testosterone response is inadequate
  • +You're transitioning to a fertility-focused protocol

Decrease if

  • -Estrogen levels rise too high (confirmed by blood work)
  • -You experience significant water retention or bloating
  • -Gynecomastia symptoms develop (tender or swelling breast tissue)
  • -Mood swings or irritability become problematic

Signs of right dose

  • Testicles maintain normal size and firmness
  • Hormone levels (testosterone, estrogen, LH) are in healthy ranges
  • Sperm production maintained or improved (if monitored)
  • No significant side effects like gynecomastia or water retention

Dosing Calculator

Calculate Your Exact Dose

Peptide in Vial

mg

BAC Water Added

2ml
ml

Desired Dose

mcg

Amount to Draw

10

units

0.0ml0.1ml0.2ml0.3ml0.4ml0.5ml0.6ml0.7ml0.8ml0.9ml1.0ml
0102030405060708090100

Concentration

2.50 mg/ml (2500 mcg/ml)

Suitability

Is this
right for me?

Best For

Fertility Preservation During TRT

If you're on testosterone therapy but want to keep your swimmers swimming, HCG is your best friend. It keeps your testes active and producing sperm even when external testosterone would normally shut that down. Research shows it can maintain or restore spermatogenesis in about 80% of men.

Preventing Testicular Atrophy

Nobody wants their boys to shrink. When you take external testosterone, your brain stops signaling your testes to work—and they get smaller from disuse. HCG mimics the signal (LH) that keeps them active, maintaining their size and function.

Hypogonadotropic Hypogonadism

If your pituitary gland isn't sending the right signals to your testes (low LH), HCG can step in and do the job. It's been used for decades to treat this condition, helping men achieve normal testosterone levels and fertility.

Post-Cycle Therapy (PCT)

After using anabolic steroids, your natural hormone production is suppressed. HCG can help jumpstart your testes back into action, speeding up the recovery of your natural testosterone production. It's often used as part of a comprehensive PCT protocol.

Consider Alternatives If

Goal: Stimulating natural testosterone without injections

Consider: Clomiphene citrate (Clomid), Enclomiphene, Gonadorelin

Goal: Fertility treatment in hypogonadal men

Consider: FSH (follicle-stimulating hormone), hMG (human menopausal gonadotropin), GnRH pulsatile therapy

Goal: Testosterone optimization without fertility concerns

Consider: Testosterone replacement therapy (TRT), Clomiphene monotherapy, Lifestyle optimization

Who Should Avoid

Do not use if

  • ×You have a hormone-sensitive cancer such as prostate cancer—HCG increases testosterone which could stimulate tumor growth
  • ×You have a history of blood clots—HCG may increase clotting risk in susceptible individuals
  • ×You're a woman trying to use HCG for weight loss—this use is not FDA approved and studies show it doesn't work
  • ×You have precocious puberty or a child with early puberty signs—HCG can accelerate pubertal development
  • ×You've had an allergic reaction to HCG or gonadotropin products before

Use with caution if

  • !You have heart disease or are at risk for cardiovascular events—monitor closely
  • !You're prone to gynecomastia—HCG can increase estrogen through aromatization
  • !You have a history of migraines—headaches may worsen initially
  • !You have kidney disease—fluid retention effects may be amplified
  • !You take blood thinners—monitor for any injection site bleeding
  • !You have epilepsy—there are rare reports of seizure threshold changes

Not Sure?

Compare Human Chorionic Gonadotropin (HCG) with similar peptides to find the best fit for your goals.

Administration

How do I
use it?

Reconstitution

What you need

  • Bacteriostatic water (BAC water)—the preservative allows multiple uses
  • Insulin syringes (29-31 gauge)—thin needles for comfortable injections
  • Alcohol swabs for cleaning vial tops and injection sites
  • Your HCG powder vial (typically 5,000 IU or 10,000 IU)

Example

If you have a 5,000 IU vial and add 2.5mL of BAC water, you get a concentration of 2,000 IU/mL. So every 0.25mL (25 units on an insulin syringe) equals 500 IU of HCG.

For a 500 IU dose at 2,000 IU/mL concentration: draw 0.25mL (25 units on a standard insulin syringe). For 1,000 IU: draw 0.5mL (50 units).

Injection

Route

Subcutaneous injection (just under the skin) or intramuscular injection—subcutaneous is easier for self-administration and equally effective

Best sites

  • Belly fat area (about 2 inches away from your belly button)
  • Front or outer thigh (middle section)
  • Upper outer buttock area (for IM injections)

Technique

  • 1.Wash your hands thoroughly with soap and water
  • 2.Clean the injection site with an alcohol swab and let it air dry
  • 3.For subcutaneous: pinch about an inch of skin to create a fold
  • 4.Insert the needle at a 45-90 degree angle (45 if you're lean, 90 if you have more tissue)
  • 5.Push the plunger slowly and steadily over 5-10 seconds
  • 6.Wait a few seconds before removing the needle
  • 7.Apply light pressure with a clean swab if needed—don't rub

Storage

Before reconstitution

Keep your HCG powder in the refrigerator (36-46°F / 2-8°C). While some products claim room temperature stability, refrigeration extends shelf life. Store in the original sealed vial away from light. Properly stored powder remains stable for 1-2 years.

After reconstitution

Once mixed with bacteriostatic water, refrigerate at 36-46°F (2-8°C). Never freeze the reconstituted solution—freezing damages the protein structure and destroys the hormone. Keep away from light. Use within 30-60 days depending on your provider's guidance.

Signs of degradation

  • Cloudy or hazy appearance (should be crystal clear)
  • Visible particles floating or settled at the bottom
  • Color changes—any discoloration means discard it
  • Clumping or precipitation that doesn't dissolve when gently swirled

Sample Daily Schedule

Morning (or whenever convenient)

250-500 IU injection

Site: Rotate between belly and thigh

Standard twice-weekly protocol: inject Monday and Thursday, or Sunday and Wednesday. Pair with your TRT injections or on alternate days—both approaches are effective. Most men use HCG continuously alongside testosterone therapy.

Safety

Is it
safe?

Safety Profile

HCG has been used medically for over 80 years with a well-established safety profile. When used appropriately for male hypogonadism or fertility preservation, side effects are generally mild and manageable. The most common issues relate to elevated estrogen from increased testosterone aromatization, which can be managed with aromatase inhibitors if needed. Serious side effects are rare when HCG is used under medical supervision.

HCG is FDA-approved for specific indications including hypogonadotropic hypogonadism in males. There's substantial clinical evidence from decades of use in fertility medicine and more recent research on its role in TRT protocols. Large real-world studies have confirmed its safety and effectiveness when properly monitored.

Common Side Effects

Experienced by some users

Injection site reactions

Mild pain, redness, or swelling at the injection site is normal and typically resolves within a day or two.

Management: Rotate injection sites regularly. Use proper technique and clean supplies. A cold compress can help if there's swelling.

Headache

Some men experience headaches, especially when starting HCG therapy. This usually improves after the first few weeks.

Management: Stay well-hydrated and use over-the-counter pain relievers if needed. If persistent, discuss with your doctor.

Water retention

Mild fluid retention can occur, causing slight bloating or puffiness. This is related to hormonal changes.

Management: Reduce sodium intake, stay hydrated, and exercise regularly. Usually mild and manageable.

Less Common

  • Gynecomastia
  • Mood changes
  • Acne

These typically resolve with continued use or dose adjustment.

Stop and Seek Help If

  • ×Signs of allergic reaction—rash, hives, swelling, difficulty breathing (stop immediately, seek emergency care)
  • ×Significant gynecomastia development that doesn't respond to estrogen management
  • ×Blood clot symptoms—sudden leg pain/swelling, chest pain, shortness of breath (stop immediately, seek emergency care)
  • ×Your fertility or TRT goals have been achieved and your doctor recommends discontinuation
  • ×Unacceptable side effects that don't improve with dose adjustments
  • ×Development of hormone-sensitive conditions

HCG is a prescription medication and should only be used under medical supervision. Never start, stop, or adjust your dosing without consulting your healthcare provider. This information is for educational purposes only—not medical advice.

Interactions

With other peptides

  • Both stimulate the reproductive axis through different mechanisms. Can be used together in some protocols but monitor hormone levels carefully.
  • Experimental combination—both affect gonadotropin release. Limited data on concurrent use.
  • No known interaction. Different mechanisms of action.

With medications

  • Testosterone (TRT) - Commonly used together. HCG helps maintain testicular function during TRT. This is a standard protocol.
  • Clomiphene citrate - Often combined for fertility protocols or PCT. Both stimulate testosterone through different pathways.
  • Aromatase inhibitors (Anastrozole) - May be needed to control estrogen that rises with HCG use. Monitor levels and dose appropriately.
  • Blood thinners (Warfarin, etc.) - May increase bruising at injection sites. Monitor INR closely as hormone changes can affect clotting.
  • Thyroid medications - HCG has weak TSH-like activity. Monitor thyroid function if on thyroid medication.

With supplements

  • DHEA - Both affect hormone levels. Use together cautiously and monitor hormone panels.
  • Zinc - Zinc supports testosterone production and is safe to take with HCG.
  • Vitamin D - Important for hormone health. Safe and potentially complementary.
  • DIM (Diindolylmethane) - May help with estrogen metabolism. Can be used alongside HCG.

Effectiveness

Does it
work?

Evidence Level

Strong human trials

What this means

HCG is a hormone that tricks your body into thinking it's receiving the signal to make testosterone. Normally, your brain releases LH (luteinizing hormone) which tells your testes to produce testosterone and keep sperm production running. When you take external testosterone, your brain stops sending that LH signal because it thinks there's enough testosterone already. HCG steps in and mimics LH, binding to the same receptors on your testes and keeping them active—producing testosterone and maintaining sperm production even when you're on TRT.

What to Expect

Week 1-2

What you might notice

  • Injection site reactions as your body gets used to the medication
  • Possible mild headaches or fatigue during adjustment
  • Some men notice improved mood or libido relatively quickly
  • Your body is beginning to respond to the LH-like stimulation

What's normal

  • Not feeling dramatic changes yet—HCG works gradually
  • Minor injection site redness or soreness that fades
  • Some water retention or mild bloating

What's next

  • Continue your prescribed protocol consistently
  • Track how you feel in a simple log
  • Side effects typically improve after the first couple weeks

Week 3-6

What you might notice

  • Testicles maintaining size or returning to fuller size
  • Stable testosterone levels when combined with TRT
  • Improved energy, libido, and overall well-being
  • Body adjusting to the hormonal changes

What's normal

  • Settling into the routine with fewer initial side effects
  • Noticeable testicular fullness compared to before HCG
  • Stable mood and energy levels

What's next

  • Blood work at 4-6 weeks to check testosterone, estrogen, and other markers
  • Dose adjustments may be made based on lab results
  • Continue monitoring for any gynecomastia signs

Week 8-12 and beyond

What you might notice

  • Sustained testicular size and function
  • For fertility goals: sperm count may be returning or maintained
  • Stable hormonal balance with good quality of life
  • Long-term benefits becoming apparent

What's normal

  • HCG becomes a routine part of your protocol
  • Consistent results with minimal side effects
  • Fertility preserved or improving (if that's your goal)

What's next

  • Regular monitoring every 3-6 months (blood work)
  • Semen analysis if fertility is the goal
  • Ongoing protocol adjustments as needed based on labs and symptoms

Not Seeing Results?

Common reasons

  • Dose too low—some men need higher doses to maintain testicular function, especially if already significantly suppressed
  • Poor quality HCG from unreliable source—always use pharmaceutical-grade product from licensed pharmacies
  • Improper storage degraded the hormone—HCG is a protein and degrades if not refrigerated or if frozen after reconstitution
  • Not enough time—testicular recovery and spermatogenesis take months, not weeks
  • Estrogen rising unchecked—high estrogen can counteract some benefits; may need an aromatase inhibitor
  • Underlying primary hypogonadism—if testes are damaged, they may not respond to HCG (it requires functioning Leydig cells)

Key Research

"Indications for the use of human chorionic gonadotropic hormone for the management of infertility in hypogonadal men"

Lee JA, Ramasamy R, 2018

Finding: This review established that HCG therapy can help re-establish or maintain spermatogenesis in hypogonadal men, making it an essential tool for fertility preservation during testosterone therapy. It highlighted HCG's role in maintaining intratesticular testosterone levels.

View Study

"Preserving fertility in the hypogonadal patient: an update"

Ramasamy R, Armstrong JM, Lipshultz LI, 2015

Finding: This study showed that HCG can reverse azoospermia caused by testosterone therapy and maintain elevated intratesticular testosterone levels. The research demonstrated that combining HCG with selective estrogen receptor modulators effectively maintains spermatogenesis in hypogonadal men.

View Study

"Gonadotropin Treatment for the Male Hypogonadotropic Hypogonadism"

Boeri L, Capogrosso P, Salonia A, 2021

Finding: This comprehensive review found that combined HCG and FSH therapy promotes testicular growth in almost all patients, spermatogenesis in approximately 80%, and pregnancy rates around 50% in men with hypogonadotropic hypogonadism. Treatment duration of 12-24 months was typical for optimal results.

View Study

"Induction of Spermatogenesis and Its Predictors in Men with Prepubertal-Onset Hypogonadotropic Hypogonadism"

Cho MC, Lee H, Kim SW, 2025

Finding: This study demonstrated that gonadotropin therapy with HCG and FSH successfully induced spermatogenesis in 82% of patients with prepubertal-onset hypogonadotropic hypogonadism. Larger baseline testicular volume was the best predictor of treatment success.

View Study

"Real-World Outcomes and Safety of Testosterone Therapy: A Longitudinal, Retrospective Cohort Study"

Clift AK, Johnson H, Huang DR, Morgentaler A, 2026

Finding: In a study of over 9,500 men, 75% received HCG as part of their testosterone therapy protocol. The combination showed a favorable safety profile with significant improvements in quality of life, sexual function, energy levels, and performance in work and sport.

View Study

Frequently Asked Questions

Medical Disclaimer

Human Chorionic Gonadotropin (HCG) is an investigational research compound not approved by the FDA for human therapeutic use. This information is for educational purposes only and should not be construed as medical advice. Always consult with a qualified healthcare provider before starting any new supplement or treatment protocol.

Last updated: 1/18/2026