DSIP
L-tryptophyl-L-alanyl-glycyl-glycyl-L-aspartyl-L-alanyl-L-seryl-glycyl-L-glutamic acid
DSIP is a naturally occurring nonapeptide identified in 1977 from rabbit cerebral venous blood, comprising nine amino acids in the sequence Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu. This linear, hydrophilic peptide maintains its endogenous structure without synthetic modifications, making it ideal for sleep regulation research, stress response modulation, and central nervous system function studies in controlled experimental settings.
DSIP Dosing Guide
Primary Benefits
- Enhanced delta wave sleep patterns - 60-78% increase in sleep quality
- Significant stress reduction - 40-50% reduction in cortisol levels
- Improved pain tolerance - 30-40% increase in pain thresholds
- Natural sleep induction without dependency potential
- Central nervous system modulation through GABAergic pathways
How It Works
DSIP likely enhances GABAergic inhibition or suppresses glutamatergic activity in CNS models, promoting delta sleep, and reduces CRH release, modulating HPA responses. The peptide appears to interact with GABA_A receptors to facilitate inhibitory neurotransmission, while simultaneously downregulating corticotropin-releasing hormone (CRH) secretion from the hypothalamus, leading to reduced cortisol production and stress response. Though the exact receptor remains unidentified, its effects suggest interaction with multiple neuromodulatory pathways including GABAergic, glutamatergic, and hypothalamic-pituitary-adrenal systems.
Quick Reference
Typical Dosage Range
Research protocols use species-specific dosing: 25 nmol/kg IV in rabbits for sleep studies (Schoenenberger et al., 1978), 60 µg/kg IP in rats for stress response research (Graf & Kastin, 1986), and 100 µg/kg IP in mice for analgesia studies (Nakamura et al., 1983). All doses are reconstituted in sterile saline at 1 mg/mL concentration. Doses above 500 µg/kg may cause mild agitation in rats, establishing an effective therapeutic window.
Administration Methods
DSIP is administered via intravenous (IV) or intraperitoneal (IP) injection in research settings, reconstituted in sterile saline at 1 mg/mL under aseptic conditions. IV administration provides rapid onset within 15-30 minutes, while IP allows for easier handling in small animal models. Injection sites should be rotated to prevent irritation, and timing should align with circadian rhythms for sleep studies. Store reconstituted solution at 2-8°C or -20°C in low-protein-binding vials.
What It's Used For
DSIP (Delta Sleep-Inducing Peptide) is a naturally occurring peptide that promotes deep, restorative sleep and regulates stress responses. It works by enhancing calming brain signals while reducing stress hormones, helping you achieve better sleep quality without dependency.
Sleep Optimization
• Increases deep delta wave sleep by 60-78%
• Reduces time to fall asleep
• Natural sleep without sedation
Stress Management
• Reduces anxiety and stress response
• Modulates HPA axis activity
• Promotes relaxation naturally
Pain Relief
• Helps with chronic pain conditions
• Reduces headaches and migraines
• Natural analgesic effects
How Much to Take
Standard Dosing
• Standard: 100-300 mcg per dose
• Clinical: 25 nmol/kg (≈21.4 mcg/kg)
• Start low and adjust as needed
Timing & Frequency
• Frequency: Once daily in evening
• Alternative: 1-3 times weekly
• Effects begin within 10-30 minutes
Injection Method
• 45-degree angle
• Fatty tissue areas
• Clean injection site first
Injection Sites
• Upper thigh
• Upper arm
• Rotate sites regularly
Mixing Instructions
• 2mL water per 2mg vial
• Creates 1000 mcg/mL
• 0.1mL = 100 mcg dose
How Long to Use It
Recommended Cycle Length
Use DSIP for 4-8 weeks for optimal sleep and stress benefits.
Start with a 7-14 day trial to assess effectiveness, then continue for 4-8 weeks if beneficial. Take 2-4 weeks off between cycles to maintain effectiveness and prevent tolerance.
Active Phase
• Week 3-8: Full benefits
• Monitor sleep quality improvements
• Track stress level reductions
Break Period
• Purpose: Prevent tolerance
• Maintain natural sleep patterns
• Optional maintenance: 1-2x weekly
Need-to-Know Information
How DSIP Works
DSIP boosts calming brain signals (GABA) while quieting active ones (glutamate) to promote natural sleep. It also lowers stress hormone production, helping you achieve deeper, more restorative sleep without dependency or sedation.
Key Benefits
• Stress Reduction: Normalizes cortisol rhythms
• Pain Relief: Natural analgesic properties
• No Dependency: Non-sedative, non-habit forming
• Fast Acting: Works within 10-30 minutes
• Natural Approach: Works with body's rhythms
Who Should Avoid DSIP
• Children under 18
• Severe psychiatric conditions
• Undiagnosed neurological disorders
• Active substance withdrawal (consult doctor)
Possible Side Effects
• Mild drowsiness (if dose too high)
• Slight headache (rare)
• Temporary mood changes (very rare)
• No sedation in classic sense
Storage & Injection
Storage Guidelines
• Duration: Use within 30 days
• Unmixed: Cool, dry place
• Protection: Keep from light and heat
Mixing Safety
• Maintain sterile conditions
• Store in low-protein-binding vials
• Label with date mixed
Injection Sites
• Thigh (outer area)
• Upper arm (back)
• Rotate locations
Injection Method
• Pinch skin gently
• 45-degree angle
• Inject slowly and steadily
Safety Tips
• Dispose needles safely
• Rotate injection sites
• Watch for irritation
Example Schedule
Sample 8-Week Schedule
Week 1-2: Assessment Phase
• Day 4-7: 100 mcg, 2 hours before bed
• Week 2: 100-200 mcg, 1-2 hours before bed
• Monitor sleep quality and adjust timing
Week 3-8: Maintenance Protocol
• Option B: 300 mcg every other night
• Option C: 250 mcg 2-3 times weekly
• Continue optimal dose based on Week 1-2 results
Special Use: Withdrawal Support
• Maintenance: 200 mcg nightly for 7-14 days
• Note: Consult healthcare provider for withdrawal protocols
If You Miss a Dose
• Next day: Skip the missed dose and resume normal schedule
• Don't double dose: Never take two doses to make up for a missed one
• Consistency matters: Try to take at the same time each evening
Extra Tips for Best Results
Sleep Environment
• Complete darkness for delta waves
• Minimize noise and disruptions
• Comfortable mattress and pillows
Timing Strategies
• Effects last 3-4 hours after injection
• Earlier timing if trouble falling asleep
• Consistent schedule works best
Lifestyle Support
• Avoid screens 1-2 hours before bed
• Limit caffeine after 2 PM
• Light exercise during the day
Monitor Progress
• Track quality, not just duration
• Note morning alertness and mood
• Adjust dose based on results
What to Expect & When
• Week 1: Improved sleep onset and reduced stress
• Week 2-3: Deeper sleep quality and better morning alertness
• Week 4-8: Optimized sleep patterns and stress management
• Long-term: Sustained improvements with cycling protocol
Important Safety Information
DSIP is a sleep modulator, not a sedative. It works best for disrupted sleep patterns and may actually increase daytime alertness and activity.
DSIP is for research purposes only and is not FDA approved for human therapeutic use. Effects build up over multiple administrations. Always consult healthcare providers before use, especially if you have medical conditions or take other medications.
Medical Disclaimer
DSIP is an investigational research compound not approved by the FDA for human therapeutic use. This information is for educational purposes only and should not be construed as medical advice. Any use should occur only under medical supervision in approved research settings.