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Injection Guide
A powerful reproductive hormone regulator that acts like the master switch for your body's fertility system—triggering the release of hormones that control everything from ovulation to testosterone production.
Route
Subcutaneous
Sites
3 recommended
Frequency
Once daily
Preparation
Bacteriostatic water (BAC water)—the preservative keeps it safe for multiple uses
Insulin syringes (29-31 gauge)—thin needles for comfortable injections
Alcohol swabs for cleaning vial tops and injection sites
Your kisspeptin-10 powder vial
Pro Tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example Calculation
If you have a 5mg (5000mcg) vial and add 2mL of BAC water, you get 2500mcg/mL. For a 100mcg dose, you'd draw 0.04mL (4 units on a 100-unit insulin syringe).
Dose Calculation
For 100mcg dose at 2500mcg/mL concentration: draw 0.04mL (4 units). For 50mcg: draw 0.02mL (2 units). Use a 0.5mL (50-unit) insulin syringe for more precise small-volume measurements.
Pro Tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 02
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Site 03
Upper Arm
Back or outer area of the upper arm. This site may require assistance from another person for proper technique.
Rotate between 3 sites to prevent tissue buildup and ensure consistent absorption.
Pro Tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by Step
Wash your hands thoroughly with soap and water
Clean the injection site with an alcohol swab and let it air dry completely
Pinch about an inch of skin to create a fold
Insert the needle at a 45-90 degree angle (45 if you're lean, 90 if you have more tissue)
Push the plunger slowly and steadily—kisspeptin is a small volume
Wait 3-5 seconds before removing the needle
Apply light pressure if needed—don't rub
Pro Tip
This peptide uses subcutaneous injection (just under the skin)—the most practical method for self-administration with good absorption. Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal Timing
Best time
Morning administration works well for most goals, as it aligns with your body's natural cortisol awakening response and gives hormones time to rise during your active hours. For fertility protocols, timing may be adjusted based on cycle phase.
With food?
Kisspeptin can be taken regardless of food timing. Injection absorption isn't significantly affected by meals, so choose whatever time works best for your routine.
Stacking notes
If combining with other peptides like PT-141 for sexual health, space injections by at least 30 minutes. Don't combine with GnRH antagonists as they directly counteract kisspeptin's mechanism. Can be used alongside clomiphene for enhanced reproductive hormone support.
Sample Daily Schedule
Morning (7-9 AM)
50-100 mcg injection
Site: Rotate between belly, thigh, and arm
Morning dosing aligns with natural cortisol rhythm and allows hormonal effects during active hours. For fertility protocols, timing may be adjusted based on cycle monitoring.
Dosing Tiers
Dose
50 mcg
Frequency
Once daily
Duration
1-2 weeks
Start low to assess your body's response. Kisspeptin works quickly—you may notice hormonal effects within hours. This dose is enough to stimulate LH release without overwhelming your system.
Dose
100 mcg
Frequency
Once daily
Duration
4-6 weeks
The commonly studied dose in clinical trials. This provides robust LH and FSH stimulation while remaining well-tolerated. In men, expect noticeable effects on testosterone within days.
Dose
100-200 mcg
Frequency
Once or twice daily
Duration
As directed by physician
Higher doses are typically used in clinical research settings, particularly for IVF protocols. The twice-daily approach (8-12 hours apart) maintains more consistent hormone stimulation. Should only be used under medical supervision.
Preservation
Before Mixing
Keep your kisspeptin powder in the refrigerator (36-46°F / 2-8°C) for short-term storage or in the freezer (-4°F / -20°C) for long-term storage. Kisspeptin-10 is a relatively stable peptide when lyophilized, but protect it from light and moisture.
After Mixing
Once mixed with bacteriostatic water, refrigerate at 36-46°F (2-8°C). Never freeze the reconstituted solution. Use within 14-21 days—kisspeptin-10's short half-life means the reconstituted peptide may degrade faster than longer peptides.
Shelf Life After Mixing
14-21 days
Signs of Degradation
Discard the vial immediately if you notice any of these:
Cloudy or hazy appearance (should be crystal clear)
Visible particles floating or settled at the bottom
Color changes—any yellowing or discoloration means discard it
Solution that smells unusual
Important
When to Stop
Any signs of allergic reaction—stop immediately and seek medical help
Persistent flushing or overheating that doesn't improve with dose reduction
Significant increases in blood pressure or heart rate
Worsening of any hormone-sensitive condition
Unexpected breast tissue changes in men (gynecomastia symptoms)
Your treatment protocol is complete or goals achieved
Kisspeptin-10 is a research compound, not an FDA-approved medication. Never start, stop, or change your dosing without guidance from a qualified healthcare provider. This information is for educational purposes only—not medical advice.
Clean Technique Checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above