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Injection Guide
The master hormone that acts as your body's repair crew—building lean muscle, burning stubborn fat, strengthening bones, and helping you recover like you did in your younger years.
Route
Subcutaneous
Sites
4 recommended
Frequency
Once daily
Preparation
Bacteriostatic water (BAC water)—the benzyl alcohol preservative keeps it sterile for multiple uses
Insulin syringes (29-31 gauge)—smaller gauge means less discomfort
Alcohol swabs for cleaning vial tops and injection sites
Your HGH powder vial
Optional: multi-use vial adapter for easier drawing
Pro Tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example Calculation
If you have a 10 IU vial and add 1mL of BAC water, you get 10 IU/mL. Each 0.1mL (10 units on an insulin syringe) equals 1 IU of HGH. For a 2 IU dose, you'd draw 0.2mL (20 units).
Dose Calculation
For a 2 IU dose at 10 IU/mL concentration: draw 0.2mL (20 units on a standard insulin syringe with 100 unit markings). Always double-check your math—HGH is expensive and you don't want to waste it or underdose.
Pro Tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 02
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Site 03
Upper Arm
Back or outer area of the upper arm. This site may require assistance from another person for proper technique.
Site 04
Gluteal
Upper outer quadrant of the buttock. This site is best for intramuscular injections and larger volumes.
Rotate between 4 sites to prevent tissue buildup and ensure consistent absorption.
Pro Tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by Step
Wash your hands thoroughly with soap and water
Let the HGH vial reach room temperature if refrigerated (5-10 minutes)
Clean the vial top and injection site with alcohol swabs
Draw your dose slowly and carefully—avoid shaking or agitating the solution
Pinch about an inch of skin and insert needle at 45-90 degree angle
Inject slowly over 5-10 seconds
Wait a few seconds before withdrawing the needle
Apply gentle pressure—don't rub the area
Pro Tip
This peptide uses subcutaneous injection (into the fat layer just under the skin)—the most common and easiest method for self-administration. Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal Timing
Best time
Most people inject first thing in the morning on an empty stomach, or before bed. Morning dosing mimics your body's natural GH pulse and avoids potential sleep disruption. Evening/bedtime dosing can enhance the natural nighttime GH surge during deep sleep.
With food?
Best taken on an empty stomach. Insulin and blood sugar spikes from food can blunt HGH's effects. Wait at least 30-60 minutes after injection before eating, especially carbohydrates. Some users inject 2-3 hours after their last meal.
Stacking notes
If combining with peptides like CJC-1295 or Ipamorelin, you can inject at the same time but use different sites. Space HGH away from meals by at least 30 minutes. If using insulin (only under medical supervision), timing becomes critical—consult a physician.
Sample Daily Schedule
Morning (immediately upon waking, 6-7 AM)
Full daily dose (e.g., 2 IU) injection
Site: Rotate between belly, thigh, and arm
This is the most popular timing. Inject on an empty stomach and wait 30-60 minutes before eating breakfast. This mimics your body's natural morning GH pulse and gives you energy for the day.
Split dosing: Morning + Bedtime
Half in AM (e.g., 1 IU), half before bed (e.g., 1 IU) injection
Site: Different sites for each injection
Some users prefer splitting their dose to maintain steadier blood levels. The nighttime dose can enhance the natural GH surge during deep sleep. This protocol may help reduce side effects from a single larger dose.
Dosing Tiers
Dose
1-2 IU
Frequency
Once daily
Duration
4-6 weeks
Start low to let your body adjust and minimize side effects like water retention and joint pain. Many people start even lower at 1 IU and slowly increase. This gentle approach helps you find your sweet spot without overwhelming your system.
Dose
2-3 IU
Frequency
Once daily
Duration
3-6 months
The most commonly used range for anti-aging, body composition, and recovery benefits. Most people find their optimal results here without significant side effects. Some split this into two daily doses (morning and evening) for steadier levels.
Dose
3-4 IU
Frequency
Once daily or split AM/PM
Duration
Ongoing with periodic breaks
Higher doses are typically used under medical supervision for more pronounced body composition changes. Side effects become more common at these levels, so careful monitoring is essential. Some users cycle 5 days on, 2 days off to maintain sensitivity.
Preservation
Before Mixing
Keep unopened HGH vials refrigerated at 36-46°F (2-8°C). Protect from light and keep in original packaging. Never freeze powder form. Most pharmaceutical HGH has an 18-24 month shelf life when properly stored. Check expiration dates.
After Mixing
Refrigerate immediately after mixing at 36-46°F (2-8°C). Never freeze reconstituted HGH—freezing denatures the protein and destroys the hormone. Avoid shaking—swirl gently if needed. Use within 28 days. Keep away from light.
Shelf Life After Mixing
28 days
Signs of Degradation
Discard the vial immediately if you notice any of these:
Cloudy or hazy solution (should be crystal clear)
Visible particles, floaters, or sediment
Any color change—should be colorless
Unusual smell or odor
Important
When to Stop
Severe or persistent headaches with vision changes—may indicate intracranial pressure
Significant swelling that doesn't improve with dose reduction
Blood sugar levels becoming diabetic range despite management
Development of new growths, lumps, or concerning symptoms
Severe joint pain or carpal tunnel that interferes with daily life
Any allergic reaction—hives, difficulty breathing, facial swelling
Human Growth Hormone is a prescription medication in most countries. This information is for educational purposes only and does not constitute medical advice. Always work with a qualified healthcare provider before starting, stopping, or adjusting HGH therapy. Regular monitoring through blood tests is essential for safe use.
Clean Technique Checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above