Immune-boosting pentapeptide fragment that enhances T-cell function and dendritic cell maturation
Written by Michael Carroll — Owner, Director of Research · Reviewed by the Peptide Initiative Research Team · Editorial standards
Subcutaneous
Route
4 recommended
Sites
Once daily
Frequency
Preparation
Thymopentin vial
Sterile saline (0.9% sodium chloride)
Sterile syringe (1 mL)
Sterile needle (25-27 gauge)
Alcohol swabs
Sterile vial for mixing
Pro tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Example calculation
For 2 mg dose: If vial contains 5 mg of thymopentin, use 0.4 mL of reconstituted solution
Dose calculation
Dose (mg) = [Desired dose ÷ Total vial amount] × Total volume mixed. Always verify concentration before injection.
Location
Site 01
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 02
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Site 03
Upper Arm
Back or outer area of the upper arm. This site may require assistance from another person for proper technique.
Site 04
Rotate sites daily to prevent irritation
Follow your provider's instructions for this injection site. Rotate sites regularly to prevent tissue damage.
Rotate between 4 sites to prevent tissue buildup and ensure consistent absorption.
Pro tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by step
Clean injection site with alcohol swab, let dry 30 seconds
Pinch skin to create fold
Insert needle at 45-90 degree angle
Push plunger slowly
Withdraw needle, apply gentle pressure with new swab
Do not massage injection site
Pro tip
This peptide uses subcutaneous (preferred) or intravenous. Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal timing
Best time
Morning injection for consistency
With food?
Can be taken with or without food; no food interactions
Stacking notes
Can be combined with other immune-supporting supplements; allow 2 hours between other injections
Sample daily schedule
Morning
1-2 mg injection
Site: Abdomen or thigh, rotate daily
Take at same time each day for consistency; can be taken with breakfast
Dosing tiers
Dose
50 mg
Frequency
Three times weekly
Duration
6 weeks to 48 weeks depending on indication
Dose used in controlled clinical trials, e.g. 50 mg SC three times weekly in HIV and atopic dermatitis studies [5].
Dose
50 mg by slow IV
Frequency
Repeated injections (e.g. three times weekly)
Duration
Several weeks
Slow intravenous infusion was used in rheumatoid arthritis trials and may enhance in-vivo potency versus bolus dosing [5].
Preservation
Before mixing
Store vial in refrigerator at 2-8°C, protect from light, keep in original packaging
After mixing
Keep reconstituted solution in sterile vial, refrigerate at 2-8°C, use within 24-48 hours
Shelf life after mixing
24-48 hours
Signs of degradation
Discard the vial immediately if you notice any of these:
Cloudy appearance
Discoloration or yellowing
Visible particles
Unusual odor
Important
When to stop
Severe allergic reaction or anaphylaxis
Persistent high fever above 102°F
Severe local injection site reaction with spreading infection signs
Signs of uncontrolled immune activation (severe fatigue, swollen lymph nodes)
Doctor's recommendation due to new medical condition
Consult healthcare provider before stopping treatment. Do not stop abruptly without medical guidance.
Clean technique checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above
Published research
Conant MA, Calabrese LH, Thompson SE, et al. · 1992
This study investigated the properties and effects of Thymopentin (TP-5), contributing to our understanding of its mechanism of action and potential therapeutic applications.
Valone FH, et al. · 2017
This study investigated the properties and effects of Thymopentin (TP-5), contributing to our understanding of its mechanism of action and potential therapeutic applications.
Fabrizi F, Dixit V, Martin P · 2006
This study investigated the properties and effects of Thymopentin (TP-5), contributing to our understanding of its mechanism of action and potential therapeutic applications.
Wolf E, Milazzo S, Boehm K, et al. · 2011
This study investigated the properties and effects of Thymopentin (TP-5), contributing to our understanding of its mechanism of action and potential therapeutic applications.
Goldstein G, et al. · 1995
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