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Injection Guide
Russian thymic peptide complex that rejuvenates immune function and supports healthy aging
Route
Intramuscular
Sites
4 recommended
Frequency
Once daily
Preparation
Thymalin lyophilized vial (10 mg)
Sterile water for injection or 0.9% sodium chloride (saline)
Sterile syringe (1-3 mL)
Sterile needle (25-27 gauge)
Alcohol swabs
Pro Tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example Calculation
For a 10 mg dose from a 10 mg vial: Add 1 mL of sterile water to the vial, creating a concentration of 10 mg/mL. Draw the full 1 mL for your 10 mg dose. For a 5 mg dose, draw 0.5 mL.
Dose Calculation
Dose (mL) = Desired dose (mg) ÷ Concentration (mg/mL). With 10 mg in 1 mL: 5 mg dose = 0.5 mL; 10 mg dose = 1.0 mL.
Pro Tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Upper Arm
Back or outer area of the upper arm. This site may require assistance from another person for proper technique.
Site 02
Gluteal
Upper outer quadrant of the buttock. This site is best for intramuscular injections and larger volumes.
Site 03
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 04
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Rotate between 4 sites to prevent tissue buildup and ensure consistent absorption.
Pro Tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by Step
Clean injection site thoroughly with alcohol swab and let dry for 30 seconds
For IM: Insert needle at 90° angle into muscle; for SC: Pinch skin and insert at 45° angle
Inject solution slowly over 5-10 seconds for comfort
Withdraw needle and apply gentle pressure with clean swab
Rotate injection sites daily to prevent tissue irritation
Pro Tip
This peptide uses intramuscular (preferred in clinical settings) or subcutaneous. Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal Timing
Best time
Morning injection for consistency; no stimulant effects so evening is also acceptable
With food?
Can be injected with or without food; food does not affect absorption
Stacking notes
If combining with Epitalon, both can be injected at the same time or spaced 2-3 hours apart; use separate injection sites
Sample Daily Schedule
Morning
5-10 mg (based on your dosing tier) injection
Site: Rotate between deltoid, gluteal, abdomen, or thigh daily
Inject at the same time each day for consistency; reconstitute fresh each day
Dosing Tiers
Dose
5 mg daily
Frequency
Once daily
Duration
5 days
Initial dose to assess tolerance and immune response; best for first-time users
Dose
10 mg daily
Frequency
Once daily
Duration
10 days
Standard clinical protocol used in most Russian research studies; most effective dose
Dose
10 mg Thymalin + 5 mg Epitalon daily
Frequency
Once daily
Duration
20 days, repeated every 6 months
Combined peptide protocol for enhanced longevity and geroprotective effects
Preservation
Before Mixing
Store lyophilized vials at 2-8°C (36-46°F) in refrigerator, protected from light, in original packaging. Can tolerate brief room temperature exposure during transport.
After Mixing
Use immediately after mixing. If not used right away, refrigerate at 2-8°C and use within 1-2 hours maximum. Do not store reconstituted solution overnight.
Shelf Life After Mixing
1-2 hours when refrigerated at 2-8°C
Signs of Degradation
Discard the vial immediately if you notice any of these:
Cloudy or hazy solution (should be clear and colorless)
Visible particles or floating matter
Unusual color change (yellowing or darkening)
Unusual odor when opening vial
Important
When to Stop
Signs of allergic reaction (swelling, difficulty breathing, widespread rash)
Persistent fever above 101°F that doesn't resolve within 24 hours
Severe injection site reaction with spreading redness or signs of infection
Your healthcare provider recommends discontinuation
Completion of prescribed course (Thymalin is used in short courses, not continuously)
Always consult your healthcare provider before starting or stopping Thymalin. This information is for educational purposes and does not replace professional medical advice. Thymalin is not FDA approved and should only be used under qualified medical supervision.
Clean Technique Checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above