Russian thymic peptide complex that rejuvenates immune function and supports healthy aging
Written by Michael Carroll — Owner, Director of Research · Reviewed by the Peptide Initiative Research Team · Editorial standards
Intramuscular
Route
4 recommended
Sites
Once daily
Frequency
Preparation
Thymalin lyophilized vial (10 mg)
Sterile water for injection or 0.9% sodium chloride (saline)
Sterile syringe (1-3 mL)
Sterile needle (25-27 gauge)
Alcohol swabs
Pro tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Example calculation
For a 10 mg dose from a 10 mg vial: Add 1 mL of sterile water to the vial, creating a concentration of 10 mg/mL. Draw the full 1 mL for your 10 mg dose. For a 5 mg dose, draw 0.5 mL.
Dose calculation
Dose (mL) = Desired dose (mg) ÷ Concentration (mg/mL). With 10 mg in 1 mL: 5 mg dose = 0.5 mL; 10 mg dose = 1.0 mL.
Location
Site 01
Upper Arm
Back or outer area of the upper arm. This site may require assistance from another person for proper technique.
Site 02
Gluteal
Upper outer quadrant of the buttock. This site is best for intramuscular injections and larger volumes.
Site 03
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 04
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Rotate between 4 sites to prevent tissue buildup and ensure consistent absorption.
Pro tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by step
Clean injection site thoroughly with alcohol swab and let dry for 30 seconds
For IM: Insert needle at 90° angle into muscle; for SC: Pinch skin and insert at 45° angle
Inject solution slowly over 5-10 seconds for comfort
Withdraw needle and apply gentle pressure with clean swab
Rotate injection sites daily to prevent tissue irritation
Pro tip
This peptide uses intramuscular (preferred in clinical settings) or subcutaneous. Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal timing
Best time
Morning injection for consistency; no stimulant effects so evening is also acceptable
With food?
Can be injected with or without food; food does not affect absorption
Stacking notes
If combining with Epitalon, both can be injected at the same time or spaced 2-3 hours apart; use separate injection sites
Sample daily schedule
Morning
5-10 mg (based on your dosing tier) injection
Site: Rotate between deltoid, gluteal, abdomen, or thigh daily
Inject at the same time each day for consistency; reconstitute fresh each day
Dosing tiers
Dose
5-10 mg reconstituted in saline
Frequency
Once daily
Duration
5-10 day course, often repeated every 6 months
Reflects the Russian clinical course used in immune-restoration and gerontology protocols, typically 10 mg IM daily for 5-10 days [1].
Dose
10 mg
Frequency
Once daily
Duration
Up to 20 day course, repeated every 6 months
Subcutaneous administration is used in community practice at the same 10 mg daily dosing; longer 20-day courses are described for cyclical immune support [1].
Preservation
Before mixing
Store lyophilized vials at 2-8°C (36-46°F) in refrigerator, protected from light, in original packaging. Can tolerate brief room temperature exposure during transport.
After mixing
Use immediately after mixing. If not used right away, refrigerate at 2-8°C and use within 1-2 hours maximum. Do not store reconstituted solution overnight.
Shelf life after mixing
1-2 hours when refrigerated at 2-8°C
Signs of degradation
Discard the vial immediately if you notice any of these:
Cloudy or hazy solution (should be clear and colorless)
Visible particles or floating matter
Unusual color change (yellowing or darkening)
Unusual odor when opening vial
Important
When to stop
Signs of allergic reaction (swelling, difficulty breathing, widespread rash)
Persistent fever above 101°F that doesn't resolve within 24 hours
Severe injection site reaction with spreading redness or signs of infection
Your healthcare provider recommends discontinuation
Completion of prescribed course (Thymalin is used in short courses, not continuously)
Always consult your healthcare provider before starting or stopping Thymalin. This information is for educational purposes and does not replace professional medical advice. Thymalin is not FDA approved and should only be used under qualified medical supervision.
Clean technique checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above
Published research
Khavinson VKh, Morozov VG · 2002
This study investigated the properties and effects of Thymalin, contributing to our understanding of its mechanism of action and potential therapeutic applications.
Gol'zand IV, Murav'eva NN, Goriacheva LG · 1986
This study investigated the properties and effects of Thymalin, contributing to our understanding of its mechanism of action and potential therapeutic applications.
Solopova IA, et al. · 2016
This study investigated the properties and effects of Thymalin, contributing to our understanding of its mechanism of action and potential therapeutic applications.
Bakhidze EV, Bokhman JaV · 1990
This study investigated the properties and effects of Thymalin, contributing to our understanding of its mechanism of action and potential therapeutic applications.
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