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Injection Guide
A brain-boosting peptide that supercharges your cognitive function by ramping up BDNF—the protein your neurons need to grow, connect, and stay sharp—all delivered through a simple nasal spray.
Route
Intranasal spray
Sites
3 recommended
Frequency
Once daily
Preparation
Bacteriostatic water (BAC water) or sterile saline for nasal use
Empty nasal spray bottle (if making your own spray)
Insulin syringes (if using subcutaneous route)
Alcohol swabs for cleaning vial tops
Your Semax powder vial
Pro Tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example Calculation
For a nasal spray: If you have a 5mg vial and want approximately 100 mcg per spray pump (standard pump delivers ~0.1mL), add 5mL of sterile solution. This gives you 1mg/mL, so each 0.1mL spray delivers 100 mcg.
Dose Calculation
For subcutaneous injection: If reconstituted at 5mg in 2mL (2.5 mg/mL), drawing 0.12 mL gives you 300 mcg. Most users prefer the nasal route for convenience and direct brain delivery.
Pro Tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Intranasal: One spray in each nostril (most common method)
Follow your provider's instructions for this injection site. Rotate sites regularly to prevent tissue damage.
Site 02
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 03
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Rotate between 3 sites to prevent tissue buildup and ensure consistent absorption.
Pro Tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by Step
For nasal spray: Gently blow your nose first to clear passages
Tilt head slightly forward (not back) to prevent throat drainage
Insert spray tip into nostril and aim toward outer wall
Press pump while inhaling gently through the nose
Avoid sniffing hard—this can push the peptide past the absorption zone
For subcutaneous: Clean site, pinch skin, inject at 45-90 degrees
Pro Tip
This peptide uses subcutaneous injection (alternative to nasal) or intranasal spray (preferred for cognitive effects). Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal Timing
Best time
Morning administration works best for most people—Semax increases alertness and focus, so taking it late in the day might interfere with sleep. If splitting doses, take the second one before 2 PM.
With food?
Semax can be taken regardless of food since it's administered nasally and absorbs directly into the brain tissue. Food timing doesn't affect its absorption or effectiveness.
Stacking notes
Semax pairs excellently with Selank for balanced cognitive and anxiolytic effects. When combining with other nootropics like Noopept, start with lower doses of each. Space different nasal peptides 15-30 minutes apart.
Sample Daily Schedule
Morning (7-10 AM)
300-600 mcg injection
Site: Intranasal (1-3 sprays per nostril depending on concentration)
Take with or without breakfast—food doesn't affect absorption. This timing maximizes cognitive benefits during your productive hours without interfering with sleep.
Early afternoon (optional second dose, before 2 PM)
200-300 mcg injection
Site: Intranasal (1-2 sprays per nostril)
If splitting daily dose, keep the second administration early enough to avoid sleep interference. Some users find a second dose helps maintain focus through the afternoon.
Dosing Tiers
Dose
200-300 mcg
Frequency
Once daily
Duration
1-2 weeks
Start low to assess your tolerance and response. The 0.1% nasal solution delivers about 50-100 mcg per drop—typically 2-3 drops per nostril. Most people notice effects within the first few days.
Dose
300-600 mcg
Frequency
Once or twice daily
Duration
3-4 weeks
The most commonly used range for cognitive enhancement. Russian clinical protocols typically use 200-600 mcg per day. Take in the morning to avoid sleep interference. Can split into morning and early afternoon doses.
Dose
600-900 mcg
Frequency
Once or twice daily
Duration
4-6 weeks
Higher doses used in clinical settings for stroke recovery and brain injury. The 1% solution (stronger formulation) is sometimes used here. Always work with a healthcare provider at these levels.
Preservation
Before Mixing
Keep your Semax powder refrigerated at 36-46°F (2-8°C) for short-term storage or frozen at -4°F (-20°C) for long-term storage. Protect from light and moisture. Properly stored powder remains stable for 2+ years when frozen.
After Mixing
Store reconstituted solution in the refrigerator at 36-46°F (2-8°C). Never freeze the reconstituted solution. Keep away from light. Nasal spray solution should be used within 30 days for optimal potency.
Shelf Life After Mixing
30 days
Signs of Degradation
Discard the vial immediately if you notice any of these:
Cloudy or hazy appearance (should be crystal clear)
Visible particles or sediment
Any color change—fresh solution should be colorless
Reduced effects compared to initial use
Important
When to Stop
Any signs of allergic reaction—stop immediately and seek medical help
Persistent headaches that don't improve after the first week
Significant blood pressure increases that concern you or your doctor
Sleep disruption that doesn't resolve with earlier dosing
Any side effect that feels serious or significantly impacts your quality of life
Your planned cycle or research protocol is complete
Semax is a research compound not FDA-approved in the United States (though it is approved in Russia). Never start, stop, or change your dosing without guidance from a qualified healthcare provider. This information is for educational purposes only—not medical advice.
Clean Technique Checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above