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Weight Management
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Healing & Recovery
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Weight Management
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Sleep & Recovery
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PNC-27
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Cognitive
Semaglutide
Weight Management
Semax
Cognitive
Sermorelin
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Total Peptides: 32
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Cognitive Protocol

SemaxComplete Dosing & Administration Guide

A brain-boosting peptide that supercharges your cognitive function by ramping up BDNF—the protein your neurons need to grow, connect, and stay sharp—all delivered through a simple nasal spray.

Dose Range

200-900mcg

Frequency

Once daily

Route

Intranasal spray

Cycle Length

4-6 weeks

Dosing

How much
do I take?

Starting Dose

200-300 mcg

Frequency

Once daily

Duration

1-2 weeks

Start low to assess your tolerance and response. The 0.1% nasal solution delivers about 50-100 mcg per drop—typically 2-3 drops per nostril. Most people notice effects within the first few days.

Standard Dose

300-600 mcg

Frequency

Once or twice daily

Duration

3-4 weeks

The most commonly used range for cognitive enhancement. Russian clinical protocols typically use 200-600 mcg per day. Take in the morning to avoid sleep interference. Can split into morning and early afternoon doses.

Advanced Dose

600-900 mcg

Frequency

Once or twice daily

Duration

4-6 weeks

Higher doses used in clinical settings for stroke recovery and brain injury. The 1% solution (stronger formulation) is sometimes used here. Always work with a healthcare provider at these levels.

Timing

Best time to take

Morning administration works best for most people—Semax increases alertness and focus, so taking it late in the day might interfere with sleep. If splitting doses, take the second one before 2 PM.

With food?

Semax can be taken regardless of food since it's administered nasally and absorbs directly into the brain tissue. Food timing doesn't affect its absorption or effectiveness.

If stacking

Semax pairs excellently with Selank for balanced cognitive and anxiolytic effects. When combining with other nootropics like Noopept, start with lower doses of each. Space different nasal peptides 15-30 minutes apart.

Adjusting Your Dose

Increase if

  • +You've tolerated the starting dose well for at least a week
  • +Cognitive benefits are subtle and you want stronger effects
  • +Your protocol calls for clinical-level dosing under supervision

Decrease if

  • -You experience persistent headaches or nasal irritation
  • -You feel overstimulated or have trouble sleeping
  • -Blood pressure noticeably increases
  • -Any uncomfortable side effects develop

Signs of right dose

  • Noticeably improved focus and concentration
  • Better memory recall throughout the day
  • Clearer thinking without feeling wired or anxious
  • Stable mood and motivation

Dosing Calculator

Calculate Your Exact Dose

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Select Weight

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Look for a number followed by 'mg' on the vial label (e.g., 5mg, 10mg)

Administration

How do I
use it?

Reconstitution

What you need

  • Bacteriostatic water (BAC water) or sterile saline for nasal use
  • Empty nasal spray bottle (if making your own spray)
  • Insulin syringes (if using subcutaneous route)
  • Alcohol swabs for cleaning vial tops
  • Your Semax powder vial

Example

For a nasal spray: If you have a 5mg vial and want approximately 100 mcg per spray pump (standard pump delivers ~0.1mL), add 5mL of sterile solution. This gives you 1mg/mL, so each 0.1mL spray delivers 100 mcg.

For subcutaneous injection: If reconstituted at 5mg in 2mL (2.5 mg/mL), drawing 0.12 mL gives you 300 mcg. Most users prefer the nasal route for convenience and direct brain delivery.

Injection

Route

Subcutaneous injection (alternative to nasal) or intranasal spray (preferred for cognitive effects)

Best sites

  • Intranasal: One spray in each nostril (most common method)
  • Belly fat area (for subcutaneous injection)
  • Front or outer thigh (for subcutaneous injection)

Technique

  • 1.For nasal spray: Gently blow your nose first to clear passages
  • 2.Tilt head slightly forward (not back) to prevent throat drainage
  • 3.Insert spray tip into nostril and aim toward outer wall
  • 4.Press pump while inhaling gently through the nose
  • 5.Avoid sniffing hard—this can push the peptide past the absorption zone
  • 6.For subcutaneous: Clean site, pinch skin, inject at 45-90 degrees
Full Injection Guide

Storage

Before reconstitution

Keep your Semax powder refrigerated at 36-46°F (2-8°C) for short-term storage or frozen at -4°F (-20°C) for long-term storage. Protect from light and moisture. Properly stored powder remains stable for 2+ years when frozen.

After reconstitution

Store reconstituted solution in the refrigerator at 36-46°F (2-8°C). Never freeze the reconstituted solution. Keep away from light. Nasal spray solution should be used within 30 days for optimal potency.

Signs of degradation

  • Cloudy or hazy appearance (should be crystal clear)
  • Visible particles or sediment
  • Any color change—fresh solution should be colorless
  • Reduced effects compared to initial use

Sample Daily Schedule

Morning (7-10 AM)

300-600 mcg injection

Site: Intranasal (1-3 sprays per nostril depending on concentration)

Take with or without breakfast—food doesn't affect absorption. This timing maximizes cognitive benefits during your productive hours without interfering with sleep.

Early afternoon (optional second dose, before 2 PM)

200-300 mcg injection

Site: Intranasal (1-2 sprays per nostril)

If splitting daily dose, keep the second administration early enough to avoid sleep interference. Some users find a second dose helps maintain focus through the afternoon.

Safety

Is it
safe?

Safety Profile

Semax has been used clinically in Russia for over 25 years with an excellent safety profile. It's approved there for treating stroke, cognitive impairment, and optic nerve diseases. Studies consistently show minimal side effects, mostly limited to mild nasal irritation when using the spray form. Unlike stimulants, Semax doesn't appear to cause tolerance, dependence, or withdrawal symptoms even with extended use.

Most human safety data comes from Russian clinical use and research. While well-documented in Russian medical literature, large-scale Western clinical trials are limited. The peptide's mechanism (BDNF enhancement) is well-understood and considered relatively safe. Always work with a healthcare provider when using research compounds.

Common Side Effects

Experienced by some users

Nasal irritation

When using the nasal spray, some users experience mild dryness, irritation, or occasional sneezing. This is the most frequently reported side effect.

Management: Use saline spray before Semax to moisturize passages. Alternate nostrils. If persistent, try lower concentration or subcutaneous route.

Mild headache

Some users report mild headaches, particularly during the first few days of use. This typically resolves as your body adjusts.

Management: Stay well-hydrated. Start with lower doses. If headaches persist beyond a week, reduce dose or discontinue.

Increased alertness

Semax promotes wakefulness and focus—which is usually the point! But this can interfere with sleep if taken too late in the day.

Management: Take all doses before 2 PM. If you're sensitive, stick to morning-only dosing.

Less Common

  • Temporary blood pressure increase
  • Dizziness

These typically resolve with continued use or dose adjustment.

Stop and Seek Help If

  • ×Any signs of allergic reaction—stop immediately and seek medical help
  • ×Persistent headaches that don't improve after the first week
  • ×Significant blood pressure increases that concern you or your doctor
  • ×Sleep disruption that doesn't resolve with earlier dosing
  • ×Any side effect that feels serious or significantly impacts your quality of life
  • ×Your planned cycle or research protocol is complete

Semax is a research compound not FDA-approved in the United States (though it is approved in Russia). Never start, stop, or change your dosing without guidance from a qualified healthcare provider. This information is for educational purposes only—not medical advice.

Interactions

With other peptides

  • Excellent pairing—Selank adds anxiolytic effects while Semax provides cognitive boost. Together they offer balanced brain enhancement. Can use both nasally, spaced 15-30 minutes apart.
  • Both enhance cognition through different mechanisms. Start with lower doses of each when combining. Monitor for overstimulation.
  • Safe to combine. BPC-157 offers healing properties while Semax provides cognitive benefits—complementary without interaction.
  • No known interactions. GHK-Cu works on tissue repair while Semax targets cognition—different pathways, safe together.

With medications

  • !Stimulant medications (Adderall, Ritalin) - Effects may be additive. Use caution and lower doses. Inform your prescriber.
  • !MAO inhibitors - Semax affects monoamine systems (dopamine, serotonin). Combining with MAOIs could cause unpredictable effects. Avoid.
  • Blood pressure medications - Semax may temporarily affect blood pressure. Monitor readings and inform your doctor.
  • Diabetes medications - Minor glucose effects noted in some diabetic patients. Monitor blood sugar more closely.

With supplements

  • Alpha-GPC - Synergistic pairing—Alpha-GPC provides choline for acetylcholine synthesis, complementing Semax's BDNF effects. Great cognitive stack.
  • Caffeine - Safe in moderate amounts. High doses may cause overstimulation when combined with Semax. Keep caffeine reasonable.
  • L-Theanine - Excellent addition—L-Theanine smooths out any jitteriness while preserving focus benefits.
  • Fish oil (Omega-3s) - Complementary for brain health. Omega-3s support neuronal membrane health alongside Semax's BDNF effects.

Want the Full Picture?

View the complete Semax research profile including mechanism of action, clinical studies, effectiveness timeline, and FAQ.

View Full Semax Profile

Medical Disclaimer

Semax is an investigational research compound not approved by the FDA for human therapeutic use. This information is for educational purposes only and should not be construed as medical advice. Always consult with a qualified healthcare provider before starting any new supplement or treatment protocol.

Last updated: 2/8/2026

Semax Protocol — Complete Dosing & Administration Guide | Peptide Initiative