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Injection Guide
A cancer-fighting peptide that works like a smart missile—it hunts down and destroys cancer cells by poking holes in them, while leaving your healthy cells completely untouched.
Route
Subcutaneous
Sites
4 recommended
Frequency
Once daily
Preparation
Bacteriostatic water (BAC water)—the preservative helps it last longer
Insulin syringes (29-31 gauge)—small needles mean less discomfort
Alcohol swabs for cleaning vial tops and injection sites
Your PNC-27 powder vial
Pro Tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example Calculation
If you have a 5mg vial and add 2.5mL of BAC water, you get a concentration of 2mg/mL. So every 0.1mL (10 units on an insulin syringe) equals 0.2mg of PNC-27.
Dose Calculation
For a 1mg dose at 2mg/mL concentration: draw 0.5mL (50 units on a standard insulin syringe). Always double-check your math before injecting!
Pro Tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 02
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Site 03
Upper Arm
Back or outer area of the upper arm. This site may require assistance from another person for proper technique.
Site 04
Flank / Love Handle
The soft tissue area on the side of the torso, above the hip. Pinch the skin and inject at a 45-degree angle.
Rotate between 4 sites to prevent tissue buildup and ensure consistent absorption.
Pro Tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by Step
Wash your hands thoroughly with soap and water
Clean the injection site with an alcohol swab and let it air dry completely
Pinch about an inch of skin to create a fold
Insert the needle at a 45-90 degree angle (45 if you're lean, 90 if you have more tissue)
Push the plunger slowly and steadily—rushing can cause discomfort
Wait 5 seconds before pulling the needle out
Don't rub the site afterward—just apply light pressure if needed
Pro Tip
This peptide uses subcutaneous injection (just under the skin)—it's the easiest method for self-administration. Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal Timing
Best time
Morning injections work well for most people—it keeps your schedule consistent and lets you monitor how you feel throughout the day.
With food?
You can take PNC-27 with or without food. It doesn't seem to matter for absorption, so do whatever fits your routine.
Stacking notes
If you're using other peptides too, inject them at different sites and wait about 30-60 minutes between each one. This helps you track which peptide causes what effect.
Sample Daily Schedule
Morning (between 7-10 AM works well for most people)
Start with 1mg, increase to 1.5-2mg based on your protocol injection
Site: Rotate between belly, thigh, and arm to prevent irritation
Pick the same time every day to build a habit. Set a phone alarm if you're forgetful. Keep a simple log of injection sites and any effects you notice.
Dosing Tiers
Dose
1 mg
Frequency
Once daily
Duration
1-2 weeks
This is where most research protocols begin. It lets you see how your body responds before moving up. Think of it as dipping your toe in the water first.
Dose
1.5 mg
Frequency
Once daily
Duration
2-4 weeks
The sweet spot for most research applications. This dose showed solid anticancer activity in lab studies without extra side effects.
Dose
2 mg
Frequency
Once daily
Duration
2-3 weeks
The higher end of research dosing. Animal studies at this level (40 mg/kg) showed powerful anti-leukemia effects. Only use under close supervision.
Preservation
Before Mixing
Keep your PNC-27 powder in the freezer (-4°F to -112°F / -20°C to -80°C). It should stay in its original sealed vial, away from light. A properly stored vial can last up to 3 years. Don't let it sit at room temperature.
After Mixing
Once you've mixed it with BAC water, move it to the refrigerator (36-46°F / 2-8°C). Never freeze the mixed solution—that destroys the peptide. Keep it away from light and use it within 14 days.
Shelf Life After Mixing
14 days
Signs of Degradation
Discard the vial immediately if you notice any of these:
Cloudy or murky appearance (it should be clear or very slightly yellow)
Particles floating around or settled at the bottom
Any unusual smell—good peptide solution has almost no odor
Color changes—if it turns dark or strange colors, toss it
Important
When to Stop
Any signs of allergic reaction—hives, swelling, trouble breathing (stop immediately and get help)
Fever that stays above 101°F for more than a day
Injection site reactions that keep getting worse instead of better
Any side effect that feels serious or really concerns you
Your research protocol is complete
Your supervising doctor or researcher tells you to stop
PNC-27 is a research compound, not an approved medication. Never start, stop, or change your dosing without guidance from a qualified medical professional or research supervisor. This information is for educational purposes—it's not medical advice.
Clean Technique Checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above