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Injection Guide
A tiny but mighty four-amino-acid peptide that activates your body's telomerase enzyme—essentially helping your cells hit the 'reset' button on aging by protecting and lengthening the protective caps on your chromosomes.
Route
Subcutaneous
Sites
3 recommended
Frequency
Once daily
Preparation
Bacteriostatic water (BAC water)—the preservative keeps it safe for multiple uses
Insulin syringes (29-31 gauge)—thin needles mean less discomfort
Alcohol swabs for cleaning vial tops and injection sites
Your Epithalon powder vial
Pro Tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example Calculation
If you have a 10mg vial and add 2mL of BAC water, you get a concentration of 5mg/mL. So every 0.2mL (20 units on an insulin syringe) equals 1mg of Epithalon.
Dose Calculation
For a 5mg dose at 5mg/mL concentration: draw 1mL (100 units on a standard insulin syringe). For a 10mg dose: draw the full 2mL or use two separate syringes.
Pro Tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 02
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Site 03
Upper Arm
Back or outer area of the upper arm. This site may require assistance from another person for proper technique.
Rotate between 3 sites to prevent tissue buildup and ensure consistent absorption.
Pro Tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by Step
Wash your hands thoroughly with soap and water
Clean the injection site with an alcohol swab and let it air dry completely
Pinch about an inch of skin to create a fold
Insert the needle at a 45-90 degree angle (45 if you're lean, 90 if you have more tissue)
Push the plunger slowly and steadily
Wait 5 seconds before removing the needle
Apply light pressure if needed—don't rub
Pro Tip
This peptide uses subcutaneous injection (just under the skin)—the easiest and most common method for self-administration. Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal Timing
Best time
Evening injections (1-2 hours before bed) work best for most people since Epithalon supports melatonin production—aligning with your natural sleep cycle makes sense.
With food?
Epithalon can be taken regardless of food timing. It doesn't seem to affect absorption either way, so fit it into whatever routine works for you.
Stacking notes
If stacking with other peptides, inject them at different sites. Epithalon pairs nicely with GHK-Cu for a comprehensive anti-aging approach. Space injections at least 30 minutes apart to track individual effects.
Sample Daily Schedule
Evening (7-9 PM, about 1-2 hours before bed)
5-10 mg depending on protocol injection
Site: Rotate between belly, thigh, and arm
Evening timing aligns with your body's natural melatonin production cycle. Follow a 10-20 day cycle, then take 3-6 months off before repeating. Most research uses 1-2 cycles per year.
Dosing Tiers
Dose
5 mg
Frequency
Once daily
Duration
10 days
This is the classic Russian protocol starting point. Inject once daily for 10 consecutive days, then take a 3-6 month break before your next cycle. Think of it as a 'cellular tune-up' you do a couple times per year.
Dose
10 mg
Frequency
Once daily
Duration
10-20 days
The most commonly used protocol in research settings. Some people split this into two cycles per year (10 days each) or do one longer 20-day cycle annually. Both approaches have been studied.
Dose
10 mg
Frequency
Once daily
Duration
20-30 days
Extended protocols for those with established tolerance. Some research has used up to 30 consecutive days. Longer doesn't always mean better—the key is consistency over years, not cramming in more per cycle.
Preservation
Before Mixing
Keep your Epithalon powder in the refrigerator (36-46°F / 2-8°C) for short-term storage or in the freezer (-4°F / -20°C) for long-term storage. Store in the original sealed vial away from light. Properly stored powder can remain stable for 2+ years when frozen.
After Mixing
Once mixed with bacteriostatic water, refrigerate at 36-46°F (2-8°C). Never freeze the reconstituted solution—freezing destroys the peptide. Keep away from light and use within 21-28 days.
Shelf Life After Mixing
21-28 days
Signs of Degradation
Discard the vial immediately if you notice any of these:
Cloudy or hazy appearance (should be crystal clear)
Visible particles floating or settled at the bottom
Color changes—any yellowing or discoloration means toss it
Unusual smell—fresh solution should have little to no odor
Important
When to Stop
Any signs of allergic reaction—stop immediately and seek medical help
Persistent headaches that don't improve after the first week
Significant sleep disturbances that worsen rather than improve
Injection site reactions that spread or don't heal
Any side effect that feels serious or really concerns you
Your research protocol/cycle is complete
Epithalon is a research compound, not an FDA-approved medication. Never start, stop, or change your dosing without guidance from a qualified healthcare provider. This information is for educational purposes only—not medical advice.
Clean Technique Checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above