Loading...
Injection Guide
Zinc-dependent thymic hormone for immune restoration and T cell maturation
Route
Subcutaneous
Sites
4 recommended
Frequency
Once daily
Preparation
Sterile bacteriostatic water (0.9% sodium chloride) or sterile saline
Insulin syringe (29-31 gauge, 0.5-1 mL)
Alcohol prep pads
Sterile gauze
Sharps container
Sealed vial with lyophilized thymulin
Pro Tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example Calculation
If vial contains 1 mg (1000 mcg) thymulin: To achieve 250 mcg per injection, reconstitute with 4 mL bacteriostatic water, then draw 1 mL for 250 mcg dose.
Dose Calculation
Total mcg in vial ÷ Total mL reconstituted = mcg per mL. Then (Desired dose in mcg ÷ mcg per mL) = mL to draw.
Pro Tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 02
Upper Arm
Back or outer area of the upper arm. This site may require assistance from another person for proper technique.
Site 03
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Site 04
Gluteal
Upper outer quadrant of the buttock. This site is best for intramuscular injections and larger volumes.
Rotate between 4 sites to prevent tissue buildup and ensure consistent absorption.
Pro Tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by Step
Clean injection site with alcohol prep pad, let dry 30 seconds
Pinch skin fold to create mound
Insert needle at 45-90 degree angle
Push plunger slowly to inject peptide
Withdraw needle and apply gentle pressure with gauze
Rotate injection sites daily to avoid lipodystrophy
Do not reuse needles
Pro Tip
This peptide uses subcutaneous injection. Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal Timing
Best time
Evening, preferably 6-8 PM to align with natural thymic hormone rhythm
With food?
Zinc supplementation should be taken with food if possible to improve absorption and reduce stomach irritation
Stacking notes
Separate thymulin injection from zinc supplement by at least 2 hours if supplementing with separate zinc dose. Can combine in reconstitution if using zinc-supplemented formulation.
Sample Daily Schedule
Evening (6-8 PM)
Starting: 100 mcg; Standard: 250-300 mcg; Advanced: 500 mcg injection
Site: Rotate between abdomen, outer thigh, upper arm, and lower back
Inject subcutaneously at same time daily. Evening timing aligns with natural thymic hormone circadian rhythm. Always use fresh, sterile needle.
Preservation
Before Mixing
Store lyophilized powder at 2-8°C (preferred) or 18-25°C in original sealed vial, protected from light and moisture. Do not freeze.
After Mixing
Store reconstituted solution at 2-8°C in sterile vial or syringe. Keep sealed to prevent contamination. Use within 5-7 days.
Shelf Life After Mixing
5-7 days when stored at 2-8°C
Signs of Degradation
Discard the vial immediately if you notice any of these:
Cloudiness or particles in solution
Color change (discoloration or darkening)
Unusual odor
Solution appears separating or layered
Important
When to Stop
Severe allergic reactions or anaphylaxis
Persistent fever above 101.5°F lasting more than 24 hours
Severe joint or muscle pain unrelated to injection site
Signs of autoimmune activation (rashes, persistent inflammation)
Severe headaches or neurological symptoms
Uncontrolled immune activation with systemic symptoms
Pregnant status or planning pregnancy
Development of new autoimmune disease symptoms
This information is for research purposes only. Stopping thymulin or any peptide should be discussed with a qualified healthcare provider. Do not discontinue abruptly without medical guidance. If experiencing emergency symptoms, seek immediate medical attention.
Clean Technique Checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above