Pharmacology
Predict plasma stability and protease susceptibility. Identify cleavage sites and get recommendations for improving peptide stability.
Example Sequences
Enter a peptide sequence to analyze stability
Minimum 3 amino acids required
Trypsin
highCleaves after Lys (K) or Arg (R), except when followed by Pro
Gastrointestinal, plasma
Chymotrypsin
highCleaves after aromatic residues (F, Y, W)
Gastrointestinal
Pepsin
mediumCleaves between hydrophobic residues
Stomach
Elastase
mediumCleaves after small aliphatic residues
Neutrophils, pancreas
Thrombin
mediumCleaves after Arg in PR-X motif
Plasma
DPP-IV
highCleaves after position 2 when Pro is at position 2
Plasma, gut epithelium
Aminopeptidase
lowFree N-terminus is a universal exopeptidase liability (blocked by N-terminal Pro or N-acetylation)
Plasma, tissues (serum exopeptidase; blocked by N-acetylation)
Carboxypeptidase
lowFree C-terminus is a universal exopeptidase liability (blocked by C-terminal Pro or C-amidation)
Plasma, tissues (serum exopeptidase; blocked by C-amidation)