Pharmacology

Half-Life Estimator

Predict plasma stability and protease susceptibility. Identify cleavage sites and get recommendations for improving peptide stability.

Peptide Sequence

0 residues

Example Sequences

Enter a peptide sequence to analyze stability

Minimum 3 amino acids required

Trypsin

high

Cleaves after Lys (K) or Arg (R), except when followed by Pro

Gastrointestinal, plasma

Chymotrypsin

high

Cleaves after aromatic residues (F, Y, W)

Gastrointestinal

Pepsin

medium

Cleaves between hydrophobic residues

Stomach

Elastase

medium

Cleaves after small aliphatic residues

Neutrophils, pancreas

Thrombin

medium

Cleaves after Arg in PR-X motif

Plasma

DPP-IV

high

Cleaves after position 2 when Pro is at position 2

Plasma, gut epithelium

Aminopeptidase

medium

Cleaves N-terminal residues (blocked by N-terminal Pro)

Plasma, tissues

Carboxypeptidase

medium

Cleaves C-terminal residues (blocked by C-terminal Pro)

Plasma, tissues