Experimental Design
Design optimized experimental assays with protocol templates, control recommendations, and sample size calculations.
4-6 hours
$5-15
High
Medium
Robust sample size. Ensure practical feasibility.
Enzyme-Linked Immunosorbent Assay for protein quantification
Applications
Key Reagents
Capture Antibody
In coating buffer (carbonate pH 9.6)
Blocking Buffer
BSA or milk in PBS
Detection Antibody
Biotinylated or HRP-conjugated
Streptavidin-HRP
If using biotinylated detection
TMB Substrate
Protect from light
Stop Solution
2N H2SO4