A groundbreaking exercise mimetic compound that tricks your body into thinking it's training for a marathon—boosting metabolism, burning fat, and building endurance without you ever having to hit the gym.
Written by Michael Carroll — Owner, Director of Research · Reviewed by the Peptide Initiative Research Team · Editorial standards
Oral
Route
3 recommended
Sites
Once daily
Frequency
Preparation
DMSO (dimethyl sulfoxide) to dissolve the powder — SLU-PP-332 does not dissolve in water
Insulin syringes (29-31 gauge) for injection
Alcohol swabs for cleaning
Oral tablets/capsules don't require reconstitution
Pro tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Example calculation
SLU-PP-332 does not dissolve in bacteriostatic water: its measured solubility in pH 7.4 phosphate-buffered saline is 0.2 μM (about 0.06 mcg/mL) [5]. In published mouse studies the powder was first dissolved in DMSO as a stock, then diluted to 5 mg/mL in 12% DMSO and 15% Cremophor in PBS for intraperitoneal injection [6].
Dose calculation
For a 500mcg dose at 5mg/mL: draw 0.1mL (10 units on a standard insulin syringe). For 1000mcg: draw 0.2mL (20 units).
Location
Site 01
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 02
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Site 03
Upper Arm
Back or outer area of the upper arm. This site may require assistance from another person for proper technique.
Rotate between 3 sites to prevent tissue buildup and ensure consistent absorption.
Pro tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by step
Wash hands thoroughly with soap and water
Clean injection site with alcohol swab, let air dry
Pinch about an inch of skin
Insert needle at 45-90 degree angle
Push plunger slowly and steadily
Wait 5 seconds, then remove needle and apply light pressure
Pro tip
This peptide uses subcutaneous injection (just under the skin) or oral administration (published mouse studies used intraperitoneal injection). Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal timing
Best time
Morning dosing aligns with your body's natural metabolic rhythm. If taking before exercise, dose 30-60 minutes prior for optimal effect. Can also split doses between morning and early afternoon.
With food?
Can be taken with or without food. Some users prefer taking with a light meal to minimize any potential gastrointestinal discomfort.
Stacking notes
If combining with other metabolic compounds, space them out and start each one separately to identify individual effects. Some users stack with L-Carnitine for enhanced fat oxidation support.
Sample daily schedule
Morning (6-9 AM)
250-500 mcg injection
Site: Oral or subcutaneous (belly area)
Morning dosing aligns with natural cortisol rhythm and metabolic activation. If exercising, take 30-60 minutes before for potential synergistic effects.
Early afternoon (12-2 PM, if splitting dose)
250-500 mcg injection
Site: Oral or rotate injection site
Second dose can help maintain consistent blood levels. Avoid dosing late in the day to prevent any potential impact on sleep.
Dosing tiers
Dose
500 mcg - 1 mg
Frequency
Once daily
Duration
8-12 weeks (research use)
No human trials exist; preclinical mice showed ERR-agonist exercise-mimetic and metabolic effects [1][2]. Dose shown is the range used in the research/biohacking community, not a validated human dose.
Preservation
Before mixing
Store powder or tablets at room temperature (59-77°F / 15-25°C) in a cool, dry place away from direct sunlight. Refrigeration extends shelf life. Keep in original sealed container with desiccant if provided.
After mixing
If using injectable form, refrigerate at 36-46°F (2-8°C). Never freeze. Keep away from light. Oral forms should remain at room temperature in original packaging.
Shelf life after mixing
28 days
Signs of degradation
Discard the vial immediately if you notice any of these:
Discoloration or yellowing of powder/solution
Cloudy appearance (solution should be clear)
Unusual odor
Clumping or crystallization of powder
Important
When to stop
Any signs of allergic reaction—stop immediately
Persistent GI issues that don't improve with dose adjustment
Significant changes in heart rate or blood pressure
Sleep problems that persist beyond the first week
Any side effect that feels concerning or unusual
Your research protocol or cycle is complete
SLU-PP-332 is a research compound, not an FDA-approved medication. Never start, stop, or change your dosing without guidance from a qualified healthcare provider. This information is for educational purposes only—not medical advice.
Clean technique checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above
Published research
Billon C, Sitaula S, Banerjee S, et al. · 2023
This foundational study showed that SLU-PP-332 increases mitochondrial function and cellular respiration in muscle cells. When given to mice, it boosted oxidative muscle fibers and enhanced exercise endurance—essentially mimicking the effects of training without the actual workout.
Billon C, Schoepke E, Avdagic A, et al. · 2024
Mice with diet-induced obesity or genetic obesity showed impressive results: SLU-PP-332 increased energy expenditure, boosted fat burning, reduced fat mass, and improved insulin sensitivity. Importantly, these effects happened without reducing appetite or food intake.
Billon C, Appourchaux K, Côté I, et al. · 2025
This study introduced an improved version (SLU-PP-915) that works when taken orally. It enhanced exercise performance and synergized with actual exercise training—meaning the compound and physical training together produced better results than either alone.
Losby M, Hayes M, Valfort A, et al. · 2024
ERR activation (the mechanism SLU-PP-332 uses) was found to regulate autophagy—the cellular cleanup process. This suggests additional anti-aging and cellular health benefits beyond just metabolic enhancement.
Okda HE, Zhao P, Hayes M, Duvall C, et al. · 2026
Medicinal-chemistry study of SLU-PP-332 analogues (Int J Biol Macromol 2026; full text PMC13112601). Measured SLU-PP-332's kinetic solubility at 0.2 μM in pH 7.4 phosphate-buffered saline, which the authors describe as extremely low and consistent with its high cLogP (4.05); several analogues kept comparable ERR activation with improved solubility or metabolic stability.
Xu W, Billon C, Li H, Wilderman A, et al. · 2024
Mouse pressure-overload heart failure study (Circulation 2024; full text PMC10842599). SLU-PP-332 improved ejection fraction, reduced fibrosis and increased survival without affecting cardiac hypertrophy. Methods: the powder was dissolved in DMSO as a stock, then diluted to 5 mg/mL in 12% DMSO and 15% Cremophor in PBS and given by intraperitoneal injection at 25 mg/kg twice daily.
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