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Injection Guide
The original growth hormone booster that works with your body's natural rhythm—it tells your pituitary gland to release more GH instead of replacing it, keeping your hormonal feedback system healthy and balanced.
Route
Subcutaneous
Sites
3 recommended
Frequency
Once daily
Preparation
Bacteriostatic water (BAC water)—the preservative allows multiple uses
Insulin syringes (29-31 gauge) for injection
Alcohol swabs for cleaning vial tops and injection sites
Your sermorelin powder vial
Pro Tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example Calculation
If you have a 5mg vial and add 2.5mL of BAC water, you get a concentration of 2mg/mL (or 2000mcg/mL). So every 0.1mL (10 units on an insulin syringe) equals 200mcg of sermorelin.
Dose Calculation
For a 300mcg dose at 2mg/mL concentration: 300mcg ÷ 2000mcg/mL = 0.15mL, which is 15 units on a standard insulin syringe.
Pro Tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 02
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Site 03
Upper Arm
Back or outer area of the upper arm. This site may require assistance from another person for proper technique.
Rotate between 3 sites to prevent tissue buildup and ensure consistent absorption.
Pro Tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by Step
Wash your hands thoroughly with soap and water
Clean the injection site with an alcohol swab and let it air dry
Pinch about an inch of skin to create a fold
Insert the needle at a 45-90 degree angle (45 if lean, 90 if more tissue)
Inject slowly and steadily over a few seconds
Wait 5-10 seconds before withdrawing the needle
Apply light pressure if needed—don't rub the site
Pro Tip
This peptide uses subcutaneous injection (just under the skin)—the standard and most effective method for sermorelin. Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal Timing
Best time
Bedtime injection (30-60 minutes before sleep) is ideal because it amplifies your body's natural nighttime GH pulse. Some protocols add a morning dose on waking for enhanced effects.
With food?
Inject on an empty stomach—avoid eating for 2-3 hours before injection. Food, especially carbohydrates, can blunt the GH response by raising insulin levels. Fasting enhances sermorelin's effectiveness.
Stacking notes
Sermorelin pairs excellently with Ipamorelin or GHRP-2/6 for synergistic GH release—they work through different pathways. If stacking, inject at the same time but at different sites. CJC-1295 (without DAC) can extend the GH pulse duration.
Sample Daily Schedule
Bedtime (30-60 minutes before sleep)
200-300 mcg injection
Site: Rotate between belly, thigh, and arm
Primary injection timing. Inject on an empty stomach for best results. This amplifies your natural nighttime GH surge during deep sleep.
Morning (optional, upon waking)
100-200 mcg injection
Site: Different site than evening injection
Optional second dose for enhanced protocols. Wait at least 30 minutes before eating. Creates an additional GH pulse to complement the nighttime release.
Dosing Tiers
Dose
100-200 mcg
Frequency
Once daily at bedtime
Duration
4-6 weeks
Start here to assess your tolerance and response. Inject about 30 minutes before bed on an empty stomach (no food for 2-3 hours). This timing syncs with your body's natural nighttime GH surge.
Dose
200-300 mcg
Frequency
Once daily at bedtime
Duration
3-6 months
The most commonly used protocol in clinical settings. Some practitioners recommend twice-daily dosing (morning and bedtime) for enhanced effects. Consistent daily use is key—skipping doses reduces effectiveness.
Dose
300-500 mcg
Frequency
Once or twice daily
Duration
3-6 months or longer
Higher doses used in some clinical studies. Twice-daily protocols (morning and evening) showed better results in research. Always work with a healthcare provider at these doses. Benefits typically plateau—more isn't always better.
Preservation
Before Mixing
Keep your sermorelin powder refrigerated at 36-46°F (2-8°C) for up to several months. For longer storage, freeze at -4°F (-20°C) or colder. Store in the original sealed vial away from light. Properly stored powder remains stable for 1-2 years.
After Mixing
Once mixed with bacteriostatic water, refrigerate at 36-46°F (2-8°C). Never freeze the reconstituted solution—freezing destroys the peptide. Keep away from light and use within 14-28 days for best potency.
Shelf Life After Mixing
14-28 days
Signs of Degradation
Discard the vial immediately if you notice any of these:
Cloudy or hazy appearance (should be crystal clear)
Visible particles or floaters in the solution
Color changes—any discoloration means discard it
Reduced effectiveness despite proper dosing
Important
When to Stop
Any signs of allergic reaction—stop immediately and seek medical help
Persistent headaches that worsen or don't improve after 2 weeks
Significant fluid retention or swelling that doesn't resolve
Development or worsening of carpal tunnel symptoms
Blood sugar control becomes difficult (if diabetic)
Your healthcare provider advises discontinuation based on labs or symptoms
Sermorelin should be used under the guidance of a qualified healthcare provider. While it has a strong safety record, it's a prescription peptide that requires proper monitoring. This information is educational—not medical advice. Always consult your doctor before starting, stopping, or changing any hormone therapy.
Clean Technique Checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above