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Fat LossMuscle BuildingInjury HealingAnti-AgingCognitive EnhancementSleep OptimizationImmune SupportGut HealingSkin RejuvenationSexual Health
Peptides
Adipotide
Weight Management
AOD-9604
Weight Management
BPC-157
Healing & Recovery
Cagrilintide
Weight Management
CJC-1295
Growth Hormone
DSIP
Sleep & Recovery
Epithalon
Anti-Aging
GHK-Cu
Anti-Aging
GHRP-2
Growth Hormone
HCG
Hormone Support
Hexarelin
Growth Hormone
HGH
Growth Hormone
IGF-1 LR3
Growth Hormone
Kisspeptin
Hormone Support
Melanotan-2
Cosmetic
MOTS-C
Metabolic
NAD+
Anti-Aging
Oxytocin Acetate
Hormone Support
PEG-MGF
Recovery
PNC-27
Cancer Research
PT-141
Sexual Health
Retatrutide
Weight Management
Selank
Cognitive
Semaglutide
Weight Management
Semax
Cognitive
Sermorelin
Growth Hormone
Snap-8
Cosmetic
SS-31
Mitochondrial
TB-500
Healing & Recovery
Tesamorelin
Growth Hormone
Thymosin Alpha-1
Immune
Tirzepatide
Weight Management
Total Peptides: 32
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Healing & Recovery Protocol

PEG-MGFComplete Dosing & Administration Guide

A supercharged version of your muscle's natural repair signal—PEGylated Mechano Growth Factor stays active for days instead of minutes, waking up dormant stem cells to rebuild and strengthen damaged muscle tissue.

Dose Range

100-200mcg

Frequency

Twice weekly

Route

Subcutaneous injection

Cycle Length

4-6 weeks

Dosing

How much
do I take?

Starting Dose

100-150 mcg

Frequency

Twice weekly

Duration

4 weeks

Start low to assess your tolerance. Inject into the muscle group you want to target, ideally after training that muscle. The PEGylation means you don't need daily injections—twice weekly is plenty.

Standard Dose

150-200 mcg

Frequency

Twice weekly

Duration

4-6 weeks

The most commonly used protocol. Many users inject bilaterally into the target muscle after training. Some split the dose between muscle groups worked that day. Take at least 4 weeks off between cycles.

Advanced Dose

200-400 mcg

Frequency

2-3 times weekly

Duration

6-8 weeks

Higher doses used by experienced users with specific recovery goals. Some protocols use daily injections at lower doses (100-150 mcg). Always cycle off—more is not always better with growth factors.

Timing

Best time to take

Inject immediately after training the target muscle group. MGF is naturally released in response to muscle damage/stress, so post-workout timing mimics your body's natural repair process.

With food?

PEG-MGF can be taken regardless of food timing. However, avoid injecting within 30-60 minutes of eating a large meal if injecting intramuscularly, as blood flow changes may affect local distribution.

If stacking

If combining with IGF-1 LR3, use PEG-MGF immediately post-workout and IGF-1 LR3 on rest days or several hours later—they work through different but complementary pathways. Some protocols alternate them.

Adjusting Your Dose

Increase if

  • +You've completed 2+ cycles at starting doses with good tolerance and want enhanced recovery
  • +Your injury or recovery demands require more aggressive support
  • +You're following a high-volume training program with significant muscle breakdown

Decrease if

  • -You experience persistent injection site reactions beyond 48 hours
  • -You notice any blood sugar irregularities
  • -Headaches or fatigue don't resolve within the first week
  • -Any unexpected or concerning side effects develop

Signs of right dose

  • Noticeably faster recovery between training sessions
  • Reduced muscle soreness after intense workouts
  • Injured areas healing more quickly than expected
  • No injection site issues beyond initial mild redness

Dosing Calculator

Calculate Your Exact Dose

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Step 1: Peptide Weight

Find the weight printed on your peptide vial label

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The weight is on the label

Peptide vial
5mg

Select Weight

mg

Look for a number followed by 'mg' on the vial label (e.g., 5mg, 10mg)

Administration

How do I
use it?

Reconstitution

What you need

  • Bacteriostatic water (BAC water)—the preservative keeps it safe for multiple uses
  • Insulin syringes (29-31 gauge)—thin needles mean less discomfort
  • Alcohol swabs for cleaning vial tops and injection sites
  • Your PEG-MGF powder vial
  • A clean, well-lit workspace

Example

If you have a 2mg (2000 mcg) vial and add 2mL of BAC water, you get a concentration of 1000 mcg/mL. So every 0.1mL (10 units on an insulin syringe) equals 100 mcg of PEG-MGF.

For a 200 mcg dose at 1000 mcg/mL concentration: draw 0.2mL (20 units on a standard insulin syringe). Always double-check your math before injecting.

Injection

Route

Subcutaneous or intramuscular injection—many users prefer intramuscular directly into the target muscle for localized effects

Best sites

  • The specific muscle group you just trained (for targeted recovery)
  • Belly fat area (about 2 inches from belly button) for subcutaneous
  • Front or outer thigh for subcutaneous
  • Deltoid (shoulder) muscle for intramuscular

Technique

  • 1.Wash your hands thoroughly with soap and water
  • 2.Clean the injection site with an alcohol swab and let it air dry
  • 3.For subcutaneous: pinch about an inch of skin and insert at 45-degree angle
  • 4.For intramuscular: insert needle straight (90 degrees) into the muscle belly
  • 5.Push the plunger slowly and steadily—don't rush
  • 6.Wait 5-10 seconds before removing the needle
  • 7.Apply light pressure with a clean swab if needed—don't rub vigorously
Full Injection Guide

Storage

Before reconstitution

Keep your PEG-MGF powder refrigerated (36-46°F / 2-8°C) for short-term storage or frozen (-4°F / -20°C) for long-term storage. Protect from light and moisture. Properly stored powder remains stable for 2+ years when frozen, 6+ months refrigerated.

After reconstitution

Once mixed with bacteriostatic water, refrigerate at 36-46°F (2-8°C). Never freeze reconstituted peptide—freezing destroys it. Keep away from light and use within 3-4 weeks for best potency.

Signs of degradation

  • Cloudy or hazy appearance (should be crystal clear)
  • Visible particles floating or settled at the bottom
  • Color changes—any yellowing or discoloration means toss it
  • Unusual smell—fresh solution should have minimal odor

Sample Daily Schedule

Immediately post-workout

100-200 mcg injection

Site: Into the muscle group trained, or subcutaneous

Inject within 30 minutes of finishing your workout for best timing with natural MGF release. For bilateral muscles (legs, arms), can split dose between both sides.

Second weekly injection (48-72 hours after first)

100-200 mcg injection

Site: Same or different target muscle

Maintain consistent twice-weekly schedule. If not training that day, subcutaneous injection is fine. The extended half-life means you don't need daily dosing.

Safety

Is it
safe?

Safety Profile

PEG-MGF (pegylated mechano growth factor) demonstrates good tolerability in preclinical studies with minimal immunogenicity from pegylation modifications. Intramuscular administration shows local tissue responses consistent with growth factor activity. No serious systemic adverse events in animal models at therapeutic doses. Pegylation extends half-life from 2 hours (native IGF-1 Ec) to 24+ hours, reducing injection frequency while maintaining safety profile similar to parent peptide.

Molecular biology validation using phospho-immunoblotting confirms IGF-1 receptor activation with dose-dependent PKB/Akt phosphorylation. Myogenic differentiation quantified through real-time PCR showing increased MyoD and myogenin expression. Muscle hypertrophy in animal models measured via MRI shows 15-20% cross-sectional area increases; satellite cell proliferation confirmed through immunohistochemistry.

Common Side Effects

Experienced by some users

Injection site irritation

A small area of redness, mild swelling, or tenderness where you injected is normal. This is your body's response to the injection and solution.

Management: Rotate injection sites between different areas. A cool compress can help. Usually resolves within 24-48 hours.

Mild headache

Some users report mild headaches in the first few days of use, possibly related to the growth factor activity.

Management: Stay well-hydrated and rest if needed. These typically resolve within the first week as your body adjusts.

Temporary fatigue

Feeling a bit tired after injection is normal, especially during the first few uses. Your body is responding to the peptide.

Management: Many users inject post-workout when they'd be resting anyway. Fatigue usually improves after the first few injections.

Less Common

  • Localized swelling
  • Blood sugar fluctuations

These typically resolve with continued use or dose adjustment.

Stop and Seek Help If

  • ×Any signs of allergic reaction—stop immediately and seek medical help
  • ×Injection site reactions that spread, worsen, or don't heal within 72 hours
  • ×Persistent headaches or fatigue that don't improve after the first week
  • ×Any blood sugar problems, especially if diabetic
  • ×Unusual lumps, growths, or tissue changes anywhere in your body
  • ×Your cycle is complete (4-8 weeks maximum)—always take at least 4 weeks off before re-dosing to prevent peptide desensitization and maintain IGF-1 pathway responsiveness

PEG-MGF is a research compound based on MGF (mechano growth factor), a naturally occurring IGF-1 splice variant, but the pegylated form is not FDA-approved. As a potent growth factor with extended half-life (48-72 hours vs. 5-7 minutes for unmodified MGF), it carries theoretical risks specific to sustained growth factor signaling. Cycling is essential to prevent receptor downregulation and potential off-target tissue growth. Never start, stop, or change your dosing without guidance from a qualified healthcare provider. This information is for educational purposes only—not medical advice. Individual responses can vary significantly based on age, training status, genetics, and overall health.

Interactions

With other peptides

  • Complementary but use different timing—PEG-MGF post-workout activates satellite cells, IGF-1 LR3 on rest days supports protein synthesis. Powerful combination when used correctly.
  • Excellent pairing for comprehensive healing. BPC-157 works on gut and systemic repair while PEG-MGF targets muscle-specific regeneration. Different mechanisms, synergistic effects.
  • Both promote healing but through different pathways. TB-500 is systemic and affects blood vessel formation, PEG-MGF is more muscle-specific. Can be used together.
  • Redundant—PEG-MGF is just a longer-acting version of MGF. Using both wastes money and doesn't add benefit. Choose one or the other.
  • Can be stacked for comprehensive growth hormone axis support. Different targets and mechanisms. Many users combine these successfully.

With medications

  • !Insulin - Potential for additive hypoglycemic effects since IGF-related peptides can lower blood sugar. Use extreme caution and monitor glucose closely.
  • !Diabetes medications (metformin, sulfonylureas) - May interact with glucose regulation. Monitor blood sugar more frequently. Consult your endocrinologist.
  • Blood thinners (warfarin, aspirin) - May increase bruising at injection sites. Not dangerous but monitor for excessive bruising. Inform your doctor.
  • Corticosteroids - Steroids can inhibit muscle repair processes. The interaction may reduce PEG-MGF effectiveness, though both can still be used if medically necessary.

With supplements

  • Creatine - Safe and potentially synergistic—creatine supports muscle energy while PEG-MGF supports repair. Common combination for athletes.
  • Protein supplements - Essential pairing—you need adequate protein for the repair processes PEG-MGF initiates. Make sure you're getting enough.
  • BCAAs - Safe to combine. BCAAs provide building blocks while PEG-MGF signals repair. Complementary mechanisms.
  • High-dose vitamin E - Very high doses of vitamin E may thin blood and increase injection site bruising. Normal supplement doses are fine.

Want the Full Picture?

View the complete PEG-MGF research profile including mechanism of action, clinical studies, effectiveness timeline, and FAQ.

View Full PEG-MGF Profile

Medical Disclaimer

PEG-MGF is an investigational research compound not approved by the FDA for human therapeutic use. This information is for educational purposes only and should not be construed as medical advice. Always consult with a qualified healthcare provider before starting any new supplement or treatment protocol.

Last updated: 2/8/2026

PEG-MGF Protocol — Complete Dosing & Administration Guide | Peptide Initiative