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Injection Guide
Your cells' master energy currency and anti-aging powerhouse—NAD+ keeps your mitochondria humming, activates longevity genes called sirtuins, and helps repair DNA damage that accumulates as you get older.
Route
Oral (NR or NMN precursors)
Sites
3 recommended
Frequency
Once daily
Preparation
NAD+ powder vial (for injectable forms)
Sterile saline or bacteriostatic water
Sterile syringes and needles
Alcohol swabs
Sterile vial for mixing
Pro Tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example Calculation
For IV infusion: A typical 250mg vial mixed with 250mL saline creates a 1mg/mL solution. The entire bag is then infused slowly over 2-4 hours to minimize flushing.
Dose Calculation
IV infusions are typically prepared by medical professionals. For oral supplements, simply take the capsule or powder as directed—no mixing required.
Pro Tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Upper Arm
Back or outer area of the upper arm. This site may require assistance from another person for proper technique.
Site 02
Upper Arm
Back or outer area of the upper arm. This site may require assistance from another person for proper technique.
Site 03
SubQ: Abdominal area (if applicable)
Follow your provider's instructions for this injection site. Rotate sites regularly to prevent tissue damage.
Rotate between 3 sites to prevent tissue buildup and ensure consistent absorption.
Pro Tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by Step
IV NAD+ should only be administered by qualified healthcare professionals
The infusion rate is crucial—too fast causes intense flushing and discomfort
Typical infusions run over 2-4 hours for 250-500mg doses
Slower rates (4+ hours) are better tolerated but require more time
Monitor for flushing, nausea, or chest tightness during infusion
Pro Tip
This peptide uses intravenous (iv) infusion is most common for direct nad+; subcutaneous or intramuscular injections are less common but sometimes used. Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal Timing
Best time
Morning is generally recommended for oral NAD+ precursors since they can boost energy and alertness. Some people find afternoon doses interfere with sleep. IV infusions can be done anytime but plan for 2-4 hours.
With food?
NAD+ precursors can be taken with or without food. Some people find taking with food reduces any digestive upset. Fat-soluble companions like resveratrol absorb better with a meal containing healthy fats.
Stacking notes
NAD+ precursors pair beautifully with sirtuin activators like resveratrol and quercetin—they work synergistically. CoQ10 and PQQ support the same mitochondrial health pathways. Space supplements 30 minutes apart if taking multiple.
Sample Daily Schedule
Morning (with breakfast)
250-500 mg oral precursor (NR or NMN) injection
Site: Oral administration
Taking in the morning supports daytime energy and avoids potential sleep interference. Can be taken with food to reduce any digestive upset. This is the most common daily protocol.
Scheduled appointment (for IV)
250-500 mg NAD+ IV infusion injection
Site: IV in arm vein
IV sessions are typically done weekly to monthly depending on goals. Plan for 2-4 hours. Eat a light meal beforehand and stay well-hydrated.
Dosing Tiers
Dose
250-300 mg NR or NMN daily
Frequency
Once daily
Duration
4-8 weeks
Start with a lower dose to see how your body responds. Most people begin with oral precursors like nicotinamide riboside (NR) or nicotinamide mononucleotide (NMN). Take in the morning with or without food.
Dose
500-1000 mg NR or NMN daily
Frequency
Once or twice daily
Duration
Ongoing
This is the most commonly used range in research studies. Some people split the dose (morning and early afternoon) for more consistent levels. Clinical trials have safely used up to 2000 mg daily.
Dose
250-500 mg IV NAD+ infusion
Frequency
Weekly to monthly
Duration
Varies by protocol
IV infusions deliver NAD+ directly into your bloodstream, bypassing digestion. Sessions typically take 2-4 hours (slower = less flushing). Often used in clinical settings for intensive protocols or specific conditions.
Preservation
Before Mixing
Store NAD+ powder in the refrigerator at 36-46°F (2-8°C). For oral supplements (NR, NMN), a cool, dry place works, but refrigeration extends shelf life. Keep away from light and moisture.
After Mixing
Reconstituted NAD+ solutions should be refrigerated and used within 14-21 days. Never freeze. Discard if solution becomes cloudy, discolored, or contains particles.
Shelf Life After Mixing
14-21 days
Signs of Degradation
Discard the vial immediately if you notice any of these:
Yellow or brown discoloration (should be clear to slightly yellow)
Cloudy appearance or floating particles
Unusual smell
Powder that won't fully dissolve
Important
When to Stop
Any signs of allergic reaction—difficulty breathing, severe rash, swelling
Persistent digestive problems that don't improve after 2 weeks
Severe or persistent headaches
Heart palpitations or significant changes in heart rhythm
If you develop any condition where NAD+ is contraindicated (like cancer diagnosis)
Side effects that concern you or affect quality of life
NAD+ supplements and infusions are not FDA-approved for treating any medical condition. The information provided is for educational purposes only. Always consult with a qualified healthcare provider before starting, stopping, or changing any supplement regimen.
Clean Technique Checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above