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Injection Guide
The shortest synthetic growth hormone-releasing peptide that tells your body to build taller and stronger.
Route
Subcutaneous
Sites
4 recommended
Frequency
Once daily
Preparation
Sterile bacteriostatic water (0.9% sodium chloride or supplied diluent)
Sterile needle and syringe (25-gauge 1mL syringe recommended)
Alcohol prep pads for sanitizing vial tops
Puncture-resistant sharps disposal container
Pro Tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example Calculation
If you weigh 70 kg and use the 30 mcg/kg dose: 70 kg × 30 mcg/kg = 2,100 mcg total daily dose. If your vial contains 5 mg (5,000 mcg) in 5mL of sterile water after reconstitution, you'd inject 2.1 mL.
Dose Calculation
Multiply your body weight in kg by 30 mcg/kg for starting dose. For standard therapy, use 100-300 mcg based on medical guidance. Concentration = total mcg in vial ÷ mL of diluent added. Your dose volume = desired mcg ÷ concentration.
Pro Tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 02
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Site 03
Upper Arm
Back or outer area of the upper arm. This site may require assistance from another person for proper technique.
Site 04
Gluteal
Upper outer quadrant of the buttock. This site is best for intramuscular injections and larger volumes.
Rotate between 4 sites to prevent tissue buildup and ensure consistent absorption.
Pro Tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by Step
Clean the injection site with an alcohol prep pad using circular motions for 30 seconds. Let dry completely (don't fan dry).
Hold the skin at the site with one hand and gently pinch to create a fold of fatty tissue.
Insert the needle at a 45-90 degree angle quickly and smoothly into the fatty layer until the needle is mostly in, but not fully.
Push the plunger slowly and steadily to inject the medication over 3-5 seconds. Keep steady pressure.
Withdraw the needle and gently massage the injection site for 10 seconds. This helps distribute the medication and reduce bruising.
Pro Tip
This peptide uses subcutaneous injection into the fatty tissue layer just under the skin. Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal Timing
Best time
30 minutes before bedtime on an empty stomach. This aligns with your body's natural nighttime GH surge.
With food?
Inject on an empty stomach. Fatty foods and high-carb meals can slightly reduce response quality.
Stacking notes
If combining with other peptides, inject sermorelin first, then wait 5-10 minutes before second injection to allow proper absorption.
Sample Daily Schedule
30 minutes before bedtime
100-300 mcg (adjust based on tier and medical guidance) injection
Site: Rotate between abdomen, thigh, and upper arm to prevent lipohypertrophy (fat buildup)
Take on an empty stomach for optimal absorption. This timing uses your body's natural nighttime growth hormone surge.
Dosing Tiers
Dose
30 mcg/kg body weight
Frequency
Once daily at bedtime
Duration
Weeks 1-2
Assess tolerance and baseline response. Lower end appropriate for initial introduction.
Dose
100-200 mcg
Frequency
Once daily at bedtime
Duration
Weeks 3-12
Most effective range for sustainable growth hormone elevation. Consistent results with minimal adaptation.
Dose
200-300 mcg
Frequency
Once daily at bedtime
Duration
8+ weeks
Higher dose range for maximum stimulation. Requires monitoring and medical supervision.
Preservation
Before Mixing
Keep your GHRH (1-29) vial in the refrigerator at 2-8°C (36-46°F). Don't freeze it. Keep it in its original box, away from direct light. Check the expiration date before each use.
After Mixing
Once you mix the powder with sterile water, it becomes even more delicate. Store the reconstituted vial upright in the refrigerator at 2-8°C immediately. Use within 24 hours of mixing for best results.
Shelf Life After Mixing
24 hours when stored properly in refrigerator
Signs of Degradation
Discard the vial immediately if you notice any of these:
Cloudy or discolored solution (should be clear)
Visible particles floating in the liquid
Vial has been at room temperature for more than 1 hour
Liquid has been stored longer than 24 hours after mixing
Important
When to Stop
Signs of allergic reaction beyond mild facial flushing (hives, swelling, difficulty breathing)
Significant worsening of existing medical conditions
Pregnancy is confirmed or planned
Medical provider advises discontinuation based on health status
Severe or persistent adverse effects that don't improve with dose adjustment
Completion of prescribed therapy course as directed by medical supervision
Always consult with your healthcare provider before stopping GHRH (1-29), especially if you're in the middle of treatment. Stopping suddenly won't cause harm, but your provider may want to monitor your progress.
Clean Technique Checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above