Loading...
Injection Guide
A naturally occurring copper peptide that acts like a master switch for your body's repair systems—boosting collagen production, accelerating wound healing, and helping your skin rebuild itself from the inside out.
Route
Subcutaneous
Sites
4 recommended
Frequency
Once daily
Preparation
Bacteriostatic water (BAC water)—the preservative keeps it safe for multiple uses
Insulin syringes (29-31 gauge)—thin needles for minimal discomfort
Alcohol swabs for cleaning vial tops and injection sites
Your GHK-Cu powder vial (may have a slight blue tint from the copper)
Pro Tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example Calculation
If you have a 5mg vial and add 2.5mL of BAC water, you get a concentration of 2mg/mL. So every 0.5mL (50 units on an insulin syringe) equals 1mg of GHK-Cu.
Dose Calculation
For a 2mg dose at 2mg/mL concentration: draw 1mL (100 units on a standard insulin syringe). The solution may have a faint blue color—this is normal and comes from the copper ion.
Pro Tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 02
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Site 03
Near the target area if treating a specific wound or scar (inject nearby, not directly into it)
Follow your provider's instructions for this injection site. Rotate sites regularly to prevent tissue damage.
Site 04
Upper Arm
Back or outer area of the upper arm. This site may require assistance from another person for proper technique.
Rotate between 4 sites to prevent tissue buildup and ensure consistent absorption.
Pro Tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by Step
Wash your hands thoroughly with soap and water
Clean the injection site with an alcohol swab and let it air dry
Pinch about an inch of skin to create a fold
Insert the needle at a 45-90 degree angle (45 if lean, 90 if more tissue)
Push the plunger slowly and steadily—rushing can cause more discomfort
Wait 5 seconds before withdrawing the needle
Apply gentle pressure with a clean cotton ball if needed—don't rub
Pro Tip
This peptide uses subcutaneous injection (just under the skin)—the most common method. can also be applied topically for localized skin benefits.. Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal Timing
Best time
Morning injections work well for GHK-Cu since it supports daytime repair processes. If using topically, apply at night so your skin can absorb it while you sleep and repair overnight.
With food?
GHK-Cu injections aren't affected by food timing, so inject whenever is convenient. If you're taking oral copper or zinc supplements, space them at least 2 hours from each other.
Stacking notes
GHK-Cu pairs beautifully with BPC-157 for a comprehensive healing stack. If injecting multiple peptides, use different injection sites and space them 15-30 minutes apart. For an anti-aging combo, pair with Epithalon—they work through completely different mechanisms.
Sample Daily Schedule
Morning (7-9 AM)
1-3 mg depending on protocol tier injection
Site: Rotate between belly, thigh, and arm (or near target area for localized healing)
Morning dosing supports your body's natural daytime repair processes. Rotate injection sites daily to prevent tissue irritation. If using for skin anti-aging, some practitioners prefer injecting in areas close to the face/neck for localized benefit.
Dosing Tiers
Dose
1 mg
Frequency
Once daily
Duration
4 weeks
Start low to see how your body responds. At this dose, you're giving your skin and tissues a gentle nudge toward repair mode. Many people notice subtle improvements in skin texture and healing speed even at this level.
Dose
2 mg
Frequency
Once daily
Duration
8-12 weeks
The sweet spot for most users. This dose has the most research support and delivers noticeable improvements in skin quality, wound healing, and potentially hair thickness. Most people tolerate it well after starting at 1mg.
Dose
3 mg
Frequency
Once daily
Duration
8-12 weeks
The upper end of commonly used doses. Some experienced users push to this level for more aggressive anti-aging or wound healing goals. Monitor for any copper-related effects and consider periodic blood work to check copper levels.
Preservation
Before Mixing
Keep your GHK-Cu powder in the refrigerator (36-46°F / 2-8°C) for short-term storage or in the freezer (-4°F / -20°C) for long-term storage. Store in the original sealed vial away from light. The powder may appear white to very faintly blue. Properly stored powder remains stable for 2+ years when frozen.
After Mixing
Once mixed with bacteriostatic water, refrigerate at 36-46°F (2-8°C). Never freeze the reconstituted solution. The solution should be clear with a possible faint blue tint. Keep away from light and use within 28 days.
Shelf Life After Mixing
28 days
Signs of Degradation
Discard the vial immediately if you notice any of these:
Solution turns dark blue or greenish (faint blue is normal, dark is not)
Cloudiness or visible particles floating in the solution
Any unusual smell—fresh solution should have little to no odor
Precipitate or crystals forming at the bottom of the vial
Important
When to Stop
Any signs of allergic reaction—stop immediately and get medical help
Persistent metallic taste that doesn't go away (possible copper excess)
Injection site reactions that spread, worsen, or show signs of infection
Nausea or stomach upset that persists beyond the first few days
Unusual skin discoloration away from injection sites
Your treatment goals have been achieved and you're in maintenance mode
GHK-Cu is a research compound, not an FDA-approved medication. Always consult with a qualified healthcare provider before starting, stopping, or adjusting any peptide protocol. This information is for educational purposes only—not medical advice.
Clean Technique Checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above