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Injection Guide
A naturally occurring brain peptide that helps you fall into deep, restorative sleep by gently nudging your body's own sleep-wake systems—without the groggy hangover of traditional sleep aids.
Route
Subcutaneous
Sites
3 recommended
Frequency
Once daily
Preparation
Bacteriostatic water (BAC water)
Insulin syringes (29-31 gauge)
Alcohol swabs
Your DSIP powder vial (typically 2mg or 5mg)
Pro Tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example Calculation
For a 2mg vial: Add 2mL of bacteriostatic water. This gives you 1mg/mL (1000 mcg/mL). For a 200mcg dose, you'll draw 0.2mL (20 units on an insulin syringe).
Dose Calculation
At 1000 mcg/mL concentration: 100mcg = 0.1mL (10 units), 200mcg = 0.2mL (20 units), 300mcg = 0.3mL (30 units). Always double-check your math!
Pro Tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 02
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Site 03
Upper Arm
Back or outer area of the upper arm. This site may require assistance from another person for proper technique.
Rotate between 3 sites to prevent tissue buildup and ensure consistent absorption.
Pro Tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by Step
Wash hands thoroughly with soap and water
Clean injection site with alcohol swab and let it dry completely
Pinch a fold of skin between thumb and forefinger
Insert needle at 45-90 degree angle (45° if lean, 90° if more tissue)
Inject slowly and smoothly over 5-10 seconds
Wait a moment, then withdraw needle and apply light pressure—don't rub
Pro Tip
This peptide uses subcutaneous injection (just under the skin)—simple and effective for self-administration. Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal Timing
Best time
Evening, 1-2 hours before your intended bedtime. DSIP doesn't knock you out instantly—it works with your body's natural sleep systems, so give it time to take effect.
With food?
Can be taken with or without food. Some people prefer taking it on an empty stomach for potentially faster absorption, but food doesn't block its effects.
Stacking notes
If using with other peptides like CJC-1295 or Ipamorelin for growth hormone support, inject at different sites. DSIP is typically taken alone in the evening while growth hormone peptides may be used at other times.
Sample Daily Schedule
Evening (1-2 hours before bedtime)
100-300 mcg based on your tier injection
Site: Abdomen or thigh—rotate sites
Take at a consistent time each evening for best results. DSIP works with your natural sleep systems, so timing matters. Aim for the same window each night, ideally 1-2 hours before you want to be asleep. A 4-6 week cycle is typical, followed by a break to assess your sleep without it.
Dosing Tiers
Dose
100 mcg
Frequency
Once daily, 1 hour before bed
Duration
1–2 weeks
Lower starting amount commonly used in practice to gauge tolerance. DSIP is research-only and has no FDA approval [1][2].
Dose
200 mcg
Frequency
Once daily, 1 hour before bed
Duration
4–6 weeks
Most commonly reported maintenance amount in practice for sleep support [1][2].
Dose
300 mcg
Frequency
Once daily, 1 hour before bed
Duration
2–4 weeks
Upper end of amounts reported in practice. Not from controlled trials, so increase cautiously [1][2].
Dose
200–300 mcg
Frequency
Once daily before bed
Duration
2–6 weeks
Intranasal use is an alternative needle-free route reported in practice; per-dose amounts are similar to the injectable route [1].
Dose
25 nmol/kg (about 1.5 mg for a 70 kg adult)
Frequency
Single slow infusion
Duration
Study setting only
Dose used in the original human sleep studies, given as a slow IV infusion under medical supervision. Improved sleep efficiency and shortened time to fall asleep [3][4].
Preservation
Before Mixing
Keep your DSIP powder in the freezer (-20°C/-4°F or colder). Store in its original sealed vial, protected from light. A properly stored vial can remain stable for up to 24 months. Never leave powder at room temperature for extended periods.
After Mixing
Once mixed with bacteriostatic water, immediately refrigerate at 36-46°F (2-8°C). Keep the vial upright and away from light. Use within 28 days. Never freeze the reconstituted solution—this destroys the peptide.
Shelf Life After Mixing
28 days
Signs of Degradation
Discard the vial immediately if you notice any of these:
Cloudy or murky solution (should be clear)
Visible particles or floaters
Any unusual color changes
Unusual smell (fresh solution has minimal odor)
Important
When to Stop
Any signs of allergic reaction—hives, swelling, breathing difficulty (stop immediately, seek help)
Severe or persistent headaches that don't respond to dose reduction
Excessive daytime drowsiness affecting your ability to function
Unusual mood changes or psychological symptoms
You've completed your planned cycle (typically 4-6 weeks)
Your healthcare provider recommends discontinuation
DSIP is a research peptide, not an FDA-approved medication. Always work with a qualified healthcare provider when using peptides. Never start, stop, or change doses without professional guidance. This information is educational—not medical advice.
Clean Technique Checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above