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Injection Guide
A modified growth hormone-releasing hormone that boosts your body's natural GH pulses for better recovery, deeper sleep, and improved body composition.
Route
Subcutaneous
Sites
3 recommended
Frequency
Once daily
Preparation
Bacteriostatic water (BAC water)
Insulin syringe (29-31 gauge)
Alcohol swabs
Sterile vial of CJC-1295 powder
Pro Tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example Calculation
2mg vial + 2mL BAC water = 1mg/mL (1000mcg/mL). This means each 0.1mL contains 100mcg.
Dose Calculation
For 100mcg dose at 1mg/mL concentration: draw 0.1mL (10 units on insulin syringe)
Pro Tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 02
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 03
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Rotate between 3 sites to prevent tissue buildup and ensure consistent absorption.
Pro Tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by Step
Clean injection site with alcohol swab and let dry
Pinch a fold of skin between thumb and forefinger
Insert needle at 45-90 degree angle into pinched skin
Inject solution slowly
Remove needle and apply light pressure (do not rub)
Pro Tip
This peptide uses subcutaneous injection into fatty tissue. Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal Timing
Best time
Before bed on empty stomach (most popular), or morning fasted and pre-workout
With food?
Take on an empty stomach - wait at least 2 hours after eating and 30-60 minutes before eating
Stacking notes
Best combined with Ipamorelin at the same time for synergistic GH release. Inject both peptides simultaneously for maximum pulse.
Sample Daily Schedule
Before bed (most common)
100-150mcg injection
Site: Abdomen
Take on empty stomach at least 2 hours after dinner. Can combine with Ipamorelin in same injection.
Morning fasted (optional second dose)
100-150mcg injection
Site: Abdomen or thigh
If using twice daily, morning dose should be fasted. Wait 30-60 minutes before eating breakfast.
Dosing Tiers
Dose
100 mcg
Frequency
Once daily before bed
Duration
2-4 weeks
Start here to assess tolerance and response. Best taken before bed to align with natural GH release during sleep.
Dose
100-150 mcg
Frequency
1-2 times daily
Duration
8-12 weeks
Most effective protocol for general health benefits. Often combined with a GHRP like Ipamorelin for enhanced effects.
Dose
150-300 mcg
Frequency
2-3 times daily
Duration
8-12 weeks
Higher dosing for experienced users seeking maximum GH stimulation. Always combined with a GHRP for synergistic effects.
Preservation
Before Mixing
Store lyophilized (freeze-dried) powder in freezer at -20C for long-term storage (years) or refrigerator at 2-8C for up to 12 months. Keep in original vial away from light and moisture.
After Mixing
Refrigerate immediately at 36-46F (2-8C). Never freeze reconstituted peptide. Keep vial upright and away from light. Use within 21-28 days.
Shelf Life After Mixing
21-28 days
Signs of Degradation
Discard the vial immediately if you notice any of these:
Cloudy or hazy solution (should be crystal clear)
Visible particles or floating matter
Solution that has been left at room temperature for extended periods
Any discoloration from the original clear appearance
Important
When to Stop
Signs of allergic reaction (hives, swelling, difficulty breathing)
Persistent severe headaches that don't improve
Significant joint pain or carpal tunnel symptoms
Development of diabetes symptoms (excessive thirst, frequent urination)
Any concerning changes in moles or skin growths
Planned surgery (stop 1-2 weeks before)
Always consult with a healthcare provider before starting or stopping peptide therapy. This information is for educational purposes and does not replace medical advice.
Clean Technique Checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above