A small-molecule NNMT inhibitor studied in animals for fat loss and NAD+ metabolism.
Written by Michael Carroll — Owner, Director of Research · Reviewed by the Peptide Initiative Research Team
Subcutaneous
Route
2 recommended
Sites
No human dose established
Frequency
Preparation
5-Amino-1MQ (oral capsules do not need mixing)
For injectable powder: bacteriostatic water
Insulin syringe
Alcohol swabs
Sterile vial
Pro tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example calculation
5-Amino-1MQ is most often supplied as oral capsules, which need no reconstitution. If you have a lyophilized (freeze-dried) powder for injection, a typical approach is to add 2 mL of bacteriostatic water to a vial containing 50 mg, giving 25 mg per mL. Note: injectable dosing for this compound has no human study behind it.
Dose calculation
With 25 mg/mL solution, a 50 mg dose would be 2 mL. Because no human dose is established, any injectable use is experimental. Capsule users simply take the labeled capsule strength.
Pro tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 02
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Rotate between 2 sites to prevent tissue buildup and ensure consistent absorption.
Pro tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by step
Most community users take capsules by mouth with water
If injecting, pinch the skin and insert at a 45-90 degree angle into the fat layer
Rotate sites to avoid irritation
No injection technique has been validated for humans with this compound
Pro tip
This peptide uses oral capsule is the most common community route; subcutaneous injection was used in animal studies [1][2]. Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal timing
Best time
Community users typically take it in the morning; no human study has tested timing.
With food?
Can be taken with or without food (community practice); no peer-reviewed guidance exists.
Stacking notes
Some community users stack it with NAD+ precursors like NMN or NR because 5-Amino-1MQ raises NAD+ by a different route [1]. This combination has never been tested together in humans.
Dosing tiers
Dose
50 – 150 mg/day (community-reported, no trial)
Frequency
Once daily, per community protocols
Duration
Undefined — no human study has run any duration
This is not a dose anyone established; it is the range people report using. The absence of human data here was checked rather than assumed: PubMed has no record under "5-amino-1MQ" and no clinical study of any NNMT inhibitor in people, ClinicalTrials.gov has no registered study of either the compound or the target, and no company has announced a clinical programme. The EU and WHO trial registries were not searched directly, so a trial registered only outside the US cannot be ruled out. With no human trial, there is no human safety data and no established human maximum, so there is nothing to describe any of these figures as tolerated against. It is shown as a single range rather than as starting, standard and advanced steps because no study set those steps — whether people using it escalate through them is a question about community practice that has not been checked here. The numbers that do exist are animal data only and do not convert to a human dose: mice received about 34 mg/kg a day by subcutaneous injection, 20 mg/kg three times daily for 11 days, n=9 [1]. In a dose-escalation study spanning 10 to 150 mg/kg/day, 60 mg/kg/day produced no observable adverse effects — in TWO mice [1]. Lab screening found off-target monoamine oxidase-A inhibition at higher concentrations, so caution with MAO-sensitive drugs and foods is reasonable [2], and the compound is cytotoxic to cultured pre-adipocytes at high concentration — about 40% loss of viability at 600 µM against none at 10 µM [1].
Preservation
Before mixing
Store capsules or sealed powder in a cool, dry place away from light. Refrigeration is recommended for powder to preserve stability.
After mixing
If reconstituted for injection, keep refrigerated at 2-8 degrees C and use within a few weeks. Do not freeze the solution.
Signs of degradation
Discard the vial immediately if you notice any of these:
Cloudiness or particles in a solution that should be clear
Color change from the normal brown/reddish tint
Any off smell
Capsules that look damp or clumped
Important
When to stop
Any severe or unexpected symptom
Signs of a reaction to MAO-A effects such as severe headache, racing heart, or spiking blood pressure [2]
Persistent nausea, headache, or fatigue
You start a medication affected by MAO-A
You become pregnant or are breastfeeding
This is an experimental research compound with no human safety data. This information is educational and not medical advice. Talk to a qualified healthcare provider before using it and before stopping any prescribed medication.
Clean technique checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above