Loading...
Injection Guide
A powerful fragment of thymosin beta-4 that acts like your body's repair signal—speeding up healing of muscles, tendons, and tissues by promoting cell migration and new blood vessel growth right where you need it most.
Route
Subcutaneous
Sites
4 recommended
Frequency
Twice weekly
Preparation
Bacteriostatic water (BAC water)—the preservative keeps it safe for multiple uses
Insulin syringes (29-31 gauge)—thin needles mean less discomfort
Alcohol swabs for cleaning vial tops and injection sites
Your TB-500 powder vial
Pro Tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example Calculation
If you have a 5mg vial and add 2mL of BAC water, you get a concentration of 2.5mg/mL. So every 1mL (100 units on an insulin syringe) equals 2.5mg of TB-500.
Dose Calculation
For a 2.5mg dose at 2.5mg/mL concentration: draw 1mL (100 units on a standard insulin syringe). For a 5mg dose: draw the full 2mL or use a larger vial reconstituted accordingly.
Pro Tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 02
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Site 03
Near the injury site (within a few inches, not directly into it)
Follow your provider's instructions for this injection site. Rotate sites regularly to prevent tissue damage.
Site 04
Upper Arm
Back or outer area of the upper arm. This site may require assistance from another person for proper technique.
Rotate between 4 sites to prevent tissue buildup and ensure consistent absorption.
Pro Tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by Step
Wash your hands thoroughly with soap and water
Clean the injection site with an alcohol swab and let it air dry completely
Pinch about an inch of skin to create a fold
Insert the needle at a 45-90 degree angle (45 if you're lean, 90 if you have more tissue)
Push the plunger slowly and steadily
Wait 5 seconds before removing the needle
Apply light pressure if needed—don't rub
Pro Tip
This peptide uses subcutaneous injection (just under the skin)—the easiest and most common method. some users prefer injecting near the injury site, though systemic administration works well too.. Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal Timing
Best time
Morning or evening—timing doesn't significantly affect TB-500's effectiveness. What matters most is consistency. Pick a schedule that works for your lifestyle and stick with it.
With food?
TB-500 can be injected regardless of food timing. It doesn't seem to affect absorption either way, so fit it into whatever routine works for you.
Stacking notes
TB-500 pairs exceptionally well with BPC-157—they're often called the 'healing stack' because they work through different but complementary mechanisms. Inject at different sites if using both. Space injections at least 15-30 minutes apart to track individual effects.
Sample Daily Schedule
Morning (or consistent time that works for you)
2-2.5 mg injection
Site: Rotate between belly, thigh, and near injury site
Inject twice weekly, spacing doses 3-4 days apart (e.g., Monday and Thursday). A typical loading phase uses 4-5 mg total per week for 4-6 weeks, followed by maintenance of 2-4 mg weekly if continued.
Dosing Tiers
Dose
2-2.5 mg
Frequency
Twice weekly
Duration
4-6 weeks
Start here to assess your tolerance and response. Many people see good results at this dose. Split your weekly dose into two injections spaced 3-4 days apart (like Monday and Thursday).
Dose
4-5 mg
Frequency
Twice weekly (split into 2-2.5 mg doses)
Duration
4-6 weeks
The most commonly used protocol for moderate injuries. Your total weekly dose is 4-5 mg, split into two injections. This is the sweet spot where most users see significant healing benefits.
Dose
5-10 mg
Frequency
Twice weekly (2.5-5 mg per injection)
Duration
4-8 weeks
Higher doses for severe or stubborn injuries. Some protocols use a loading phase of higher doses for 1-2 weeks, then drop to maintenance. Work with a provider for doses above 5 mg weekly.
Preservation
Before Mixing
Keep your TB-500 powder in the refrigerator (36-46°F / 2-8°C) for short-term storage or in the freezer (-4°F / -20°C) for long-term storage. Store in the original sealed vial away from light. Properly stored powder can remain stable for 2+ years when frozen.
After Mixing
Once mixed with bacteriostatic water, refrigerate at 36-46°F (2-8°C). Never freeze the reconstituted solution—freezing destroys the peptide. Keep away from light and use within 28 days.
Shelf Life After Mixing
28 days
Signs of Degradation
Discard the vial immediately if you notice any of these:
Cloudy or hazy appearance (should be crystal clear)
Visible particles floating or settled at the bottom
Color changes—any yellowing or discoloration means toss it
Unusual smell—fresh solution should have little to no odor
Important
When to Stop
Any signs of allergic reaction—stop immediately and seek medical help
Persistent headaches or fatigue that don't improve after the first week
Significant injection site reactions that spread or don't heal
Any side effect that feels serious or really concerns you
Your injury has healed satisfactorily
Your research protocol/cycle is complete
TB-500 is a research compound, not an FDA-approved medication. Never start, stop, or change your dosing without guidance from a qualified healthcare provider. This information is for educational purposes only—not medical advice.
Clean Technique Checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above