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Metabolic Protocol

Xenin-25Complete Dosing & Administration Guide

The Natural Incretin Helper Peptide That Enhances Glucose Control and Insulin Response

Dose Range

0.1 nmol/kg-1.0 nmol/kgnmol/kg

Frequency

Once daily

Route

Intravenous (IV)

Cycle Length

Ongoing/indefinite

Dosing

How much
do I take?

Timing

Best time to take

Xenin-25 is administered intravenously in a clinical setting. Timing is determined by your healthcare provider based on the treatment protocol and your medical needs.

With food?

IV administration of Xenin-25 is not dependent on meal timing. Your healthcare team will provide specific instructions regarding food and fluid intake around treatment sessions.

If stacking

Xenin-25 should be used as directed by your healthcare provider. If combining with other medications or supplements, discuss potential interactions with your provider. Avoid combining with compounds that have overlapping mechanisms unless specifically guided by a medical professional.

Adjusting Your Dose

Increase if

  • +You've tolerated the current dose for the recommended period without significant side effects
  • +Therapeutic goals haven't been met at the current dose level
  • +Your healthcare provider recommends dose escalation based on your response
  • +Lab work or clinical assessments support a higher dose

Decrease if

  • -Side effects are bothersome or impacting daily life despite management strategies
  • -You experience any signs of an adverse reaction
  • -Lab results indicate the need for dose reduction
  • -Your healthcare provider recommends a lower dose based on your response

Signs of right dose

  • Therapeutic goals being met with minimal side effects
  • Stable and consistent response to treatment
  • Lab values or clinical markers trending in the right direction
  • Good tolerance with manageable or absent side effects

Dosing Calculator

Calculate Your Exact Dose

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Step 1: Peptide Weight

Find the weight printed on your peptide vial label

Look here!

The weight is on the label

Peptide vial
5mg

Select Weight

mg

Look for a number followed by 'mg' on the vial label (e.g., 5mg, 10mg)

Administration

How do I
use it?

Reconstitution

What you need

  • Xenin-25 vial (lyophilized powder or solution)
  • Bacteriostatic water or sterile sodium chloride for reconstitution
  • Alcohol swabs for cleaning vial tops and injection sites
  • Appropriately sized syringes with fine-gauge needles (27-30 gauge)
  • Sharps disposal container

Example

Add the recommended volume of bacteriostatic water to the Xenin-25 vial. Gently swirl (do not shake) until the powder is fully dissolved. The resulting solution should be clear. Calculate your individual dose based on the concentration and your prescribed amount.

Your dose of Xenin-25 is determined by your healthcare provider. Using an insulin syringe marked in units, draw up the exact amount prescribed. For example, if the reconstituted concentration is 1mg/mL and your dose is 0.5mg, draw up 0.5mL (50 units on an insulin syringe). Always double-check calculations before injection.

Injection

Route

Subcutaneous injection (into the fatty tissue just under the skin)—allows for consistent absorption and can be self-administered at home after proper training

Best sites

  • Abdomen (stomach area)—at least 2 inches from the belly button, most popular choice for self-injection
  • Front of thighs—middle to upper portion of the outer leg
  • Back of upper arm—outer area (may need assistance from another person)

Technique

  • 1.Wash your hands thoroughly with soap and water before handling supplies
  • 2.Clean the injection site with an alcohol swab and let it air dry completely
  • 3.Pinch a fold of skin at the chosen injection site
  • 4.Insert the needle at a 45-90 degree angle (depending on needle length and body composition)
  • 5.Inject the medication slowly and steadily over 5-10 seconds
  • 6.Release the skin fold and remove the needle, applying gentle pressure with a clean swab
  • 7.Rotate injection sites to prevent tissue irritation or lipodystrophy
  • 8.Dispose of the needle safely in a sharps container—never recap or reuse needles

Storage

Before reconstitution

Store Xenin-25 in the refrigerator at 36-46°F (2-8°C) in its original packaging. Protect from light and moisture. Do not freeze. Check the expiration date before use. Some formulations may be stored at room temperature for limited periods—check your specific product labeling.

After reconstitution

Once reconstituted, Xenin-25 should be kept refrigerated at 36-46°F (2-8°C) and used within the timeframe specified on your product labeling (typically 14-28 days). Label the vial with the reconstitution date. Do not use if the solution appears cloudy, discolored, or contains particles.

Signs of degradation

  • Solution appears cloudy, discolored, or contains visible particles (should be clear)
  • Product has been exposed to temperatures outside the recommended storage range
  • Product has been frozen (unless specifically designed for freeze-thaw stability)
  • Expiration date has passed or reconstituted solution has exceeded its use-by date
  • Unusual odor, color change, or visible contamination

Sample Daily Schedule

As prescribed (once daily)

As prescribed by your healthcare provider injection

Site: Intravenous (IV)—rotate sites if applicable

Maintain a consistent schedule for optimal results with Xenin-25. Set reminders if needed. If you miss a dose, follow your healthcare provider's instructions—do not double up on doses to compensate.

Safety

Is it
safe?

Safety Profile

Xenin-25 is a 25-amino acid natural gut peptide discovered in 1992 with moderate evidence from Phase 1-2 human clinical trials. The peptide has been extensively studied in metabolic research for over 30 years. Safety profile shows good tolerability in research settings with mild, transient gastrointestinal effects being the most common. Critical limitation: Xenin-25 has NOT completed Phase 3 clinical trials and is not FDA-approved for clinical use. No serious adverse effects have been reported in research studies, but hypoglycemia risk requires careful monitoring when combined with insulin or other glucose-lowering agents. Individual responses to Xenin-25 are generally mild gastrointestinal discomfort, nausea, or headache in the first few days—these typically resolve with continued use. The peptide's glucose-dependent mechanism makes hypoglycemia unlikely when used alone, but additive effects with other agents require medical supervision.

Xenin-25's evidence base spans over 30 years of investigation since its 1992 discovery as a neurotensin-related gut peptide. Phase 1-2 clinical data demonstrates its role as a glucose-dependent insulin secretagogue with additive effects when combined with GIP. Human tolerability studies show a favorable safety profile at therapeutic doses, though the peptide is rapidly degraded by DPP-IV, limiting its clinical utility without stabilization modifications.

Common Side Effects

Experienced by some users

Gastrointestinal Discomfort

Some users experience mild stomach cramping, bloating, or digestive changes, especially in the first few days of use. This typically subsides quickly.

Management: Take with meals, stay hydrated, and give your body 3-5 days to adjust. Slow dose escalation helps minimize GI symptoms.

Nausea

Light nausea can occur, particularly with initial doses. It's usually mild and temporary.

Management: Start with lower doses and increase gradually. Take with food. Most users report improvement after first week.

Headache

Some individuals report mild headaches, usually in the first few days of treatment.

Management: Ensure proper hydration, get adequate sleep, and consider over-the-counter pain relievers if needed. Typically resolves on its own.

Less Common

  • Injection Site Reactions
  • Mild Fatigue

These typically resolve with continued use or dose adjustment.

Stop and Seek Help If

  • ×Severe or worsening side effects that don't improve with dose adjustment or supportive care
  • ×Signs of an allergic reaction—rash, hives, swelling, or difficulty breathing
  • ×Your healthcare provider recommends discontinuation based on your clinical response
  • ×Development of any new medical condition that may be contraindicated with Xenin-25
  • ×Pregnancy or planning to become pregnant (unless specifically approved for use during pregnancy)
  • ×Abnormal lab results or clinical markers that suggest adverse effects

Xenin-25 should only be started, adjusted, or discontinued under medical supervision. This information is for educational purposes only and does not replace professional medical advice. Never stop a prescribed treatment without consulting your healthcare provider first, as abrupt discontinuation may have consequences.

Interactions

With other peptides

  • Both secreted from same K-cells; show synergistic glucose control benefits when combined
  • Complementary incretin hormone mechanisms enhance overall glucose homeostasis
  • DPP-IV inhibitors preserve xenin activity and enhance combined metabolic effects

With medications

  • !Insulin (without professional medical monitoring) - Risk of hypoglycemia without proper glucose monitoring and dose adjustment

With supplements

  • Multivitamins - Generally safe to take alongside Xenin-25. Space doses apart if taking oral formulations to ensure optimal absorption.
  • Electrolyte supplements - Helpful if experiencing any GI side effects that could lead to dehydration. Safe to combine.

Want the Full Picture?

View the complete Xenin-25 research profile including mechanism of action, clinical studies, effectiveness timeline, and FAQ.

View Full Xenin-25 Profile

Medical Disclaimer

Xenin-25 is an investigational research compound not approved by the FDA for human therapeutic use. This information is for educational purposes only and should not be construed as medical advice. Always consult with a qualified healthcare provider before starting any new supplement or treatment protocol.

Last updated: 2/8/2026