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Injection Guide
A powerful hexapeptide that stimulates growth hormone release—used clinically in Japan for GH testing and studied for healing and anti-inflammatory effects.
Route
Subcutaneous
Sites
3 recommended
Frequency
Once daily
Preparation
Lyophilized GHRP-2 vial (typically 5-10 mg per vial)
Bacteriostatic water (0.9% benzyl alcohol saline solution)
Insulin syringe (29-31 gauge, 1 mL)
Alcohol swabs (70% isopropyl)
Sterile needle for drawing bacteriostatic water
Sterile needle for injection
Pro Tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example Calculation
If you receive a 5 mg vial and want 100 mcg (0.1 mg) per injection: Mix entire 5 mg vial with 50 mL bacteriostatic water. This creates a concentration of 0.1 mg/mL, so 1 mL (on the 100-mark of your insulin syringe) = 100 mcg.
Dose Calculation
Formula: (Total mg in vial × 1000 mcg) ÷ mL of bacteriostatic water = mcg per mL. Example: (5 mg × 1000) ÷ 50 mL = 100 mcg/mL. To draw 100 mcg, pull to the 100-mark on a 1 mL (100 unit) insulin syringe.
Pro Tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 02
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Site 03
Upper Arm
Back or outer area of the upper arm. This site may require assistance from another person for proper technique.
Rotate between 3 sites to prevent tissue buildup and ensure consistent absorption.
Pro Tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by Step
Clean injection site with alcohol swab in circular motion, allow to air dry (5-10 seconds)
Pinch a fold of skin and fat between thumb and forefinger to lift tissue away from muscle
Insert needle at 45-90 degree angle into the pinched fold (should be painless if done correctly)
Push plunger slowly and steadily to inject the full dose (takes 3-5 seconds)
Withdraw needle, release skin fold, and apply gentle pressure with fresh alcohol swab for 10 seconds
Pro Tip
This peptide uses subcutaneous injection into fatty tissue (adipose layer between skin and muscle). Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal Timing
Best time
Administer on an empty stomach (2+ hours after meals, 30+ minutes before eating) to maximize GH response. Fasting state enhances peptide efficacy.
With food?
Food intake, especially carbohydrates and amino acids, blunts the GH response. Maintain a gap of at least 2 hours post-meal for optimal effect.
Stacking notes
If combining with CJC-1295, space injections 15-30 minutes apart for synergistic effect. Avoid injecting into the same body site within 48 hours.
Sample Daily Schedule
Morning (upon waking, fasted)
100-200 mcg injection
Site: Lower abdomen
Administer after 8+ hours of fasting for maximum GH response. Eat 30+ minutes after injection.
Pre-workout (if using twice daily)
100-200 mcg injection
Site: Outer thigh
Inject 30-60 minutes before resistance training on non-fasting days for enhanced muscle protein synthesis during workout.
Evening (optional third dose)
100-200 mcg injection
Site: Back of upper arm
Some advanced users inject before bed on fasted stomachs to capitalize on natural nighttime GH peaks. Not typical—reserve for intensive protocols.
Dosing Tiers
Dose
50-100 mcg
Frequency
Once daily or twice weekly
Duration
1-2 weeks
Begin conservatively to assess individual responsiveness and side effect tolerance. Administer subcutaneously in abdomen or thigh.
Dose
100-200 mcg
Frequency
1-3 times daily
Duration
4-6 weeks
Most individuals find optimal response and tolerability in this range. Typical use case for GH axis stimulation and recovery support.
Dose
200-300 mcg
Frequency
2-3 times daily
Duration
4-8 weeks
Higher doses used in intensive research or competitive athletic settings. Requires previous GHRP experience and close monitoring. Risk-benefit ratio increases.
Preservation
Before Mixing
Keep lyophilized powder in freezer at -20°C or refrigerator at 4°C. Avoid repeated temperature fluctuations. Store in original vial protected from light and moisture.
After Mixing
Refrigerate reconstituted solution at 2-8°C (36-46°F). Do NOT freeze reconstituted peptide. Use within 28 days of reconstitution. Keep vial capped and protected from light.
Shelf Life After Mixing
28 days
Signs of Degradation
Discard the vial immediately if you notice any of these:
Solution becomes cloudy or discolored (indicates bacterial growth or oxidation)
Visible particles or crystals form in solution
Strong chemical odor emerges (suggests degradation)
Solution appears darker than initially prepared (oxidation from light or heat exposure)
Important
When to Stop
Severe or persistent headaches that worsen despite dose reduction
Uncontrolled blood pressure elevation during dosing windows
Development of new joint pain or swelling that interferes with daily function
Signs of allergic reaction (rash, swelling, difficulty breathing)
Any signs of infection at injection site (increasing redness, warmth, drainage)
Planned surgery or acute hospitalization (discuss with medical team first)
This list is for educational purposes only and does not replace medical judgment. Always consult with a healthcare provider before starting, modifying, or stopping any peptide protocol. Pralmorelin is not FDA approved and should only be used under qualified medical supervision or within approved research settings.
Clean Technique Checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above