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Injection Guide
A uniquely selective growth hormone booster that tells your pituitary to release more GH without messing with your cortisol or other hormones—making it the 'clean' choice among growth hormone peptides.
Route
Subcutaneous
Sites
3 recommended
Frequency
Multiple times daily
Preparation
Bacteriostatic water (BAC water)—the preservative allows multiple uses
Insulin syringes (29-31 gauge, 0.5mL or 1mL)
Alcohol swabs for cleaning vial tops and injection sites
Your Ipamorelin powder vial (typically 2mg or 5mg)
Pro Tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example Calculation
For a 5mg vial: Add 2.5mL of bacteriostatic water to get a concentration of 2mg/mL (2000 mcg/mL). Each 0.1mL (10 units on an insulin syringe) equals 200 mcg of Ipamorelin.
Dose Calculation
At 2mg/mL concentration: For a 200 mcg dose, draw 0.1mL (10 units). For 300 mcg, draw 0.15mL (15 units). For 100 mcg, draw 0.05mL (5 units).
Pro Tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 02
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Site 03
Upper Arm
Back or outer area of the upper arm. This site may require assistance from another person for proper technique.
Rotate between 3 sites to prevent tissue buildup and ensure consistent absorption.
Pro Tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by Step
Wash your hands thoroughly with soap and water
Clean the injection site with an alcohol swab and let it air dry
Pinch about an inch of skin to create a fold
Insert the needle at a 45-90 degree angle (45 for lean individuals, 90 if you have more tissue)
Push the plunger slowly and steadily over 5-10 seconds
Wait 5 seconds before removing the needle to ensure full delivery
Apply light pressure with a cotton ball if needed—don't rub the site
Pro Tip
This peptide uses subcutaneous injection (just under the skin)—quick, easy, and most effective for peptides like ipamorelin. Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal Timing
Best time
Inject on an empty stomach—ideally first thing in the morning, post-workout, and before bed. The bedtime dose aligns with your natural overnight GH surge. Wait at least 30 minutes before eating after injection.
With food?
Food, especially carbs and fats, can blunt the GH response. Always inject at least 30-60 minutes before meals or 2-3 hours after eating for best results.
Stacking notes
When pairing with CJC-1295, inject them together at the same time—they work synergistically. If using BPC-157 or TB-500 for healing, you can inject those at different sites or times.
Sample Daily Schedule
Morning (upon waking, fasted)
100-300 mcg depending on protocol injection
Site: Rotate: belly, thigh, or arm
First thing in the morning on an empty stomach. Wait 30 minutes before eating breakfast to maximize GH pulse.
Post-workout (if training that day)
100-300 mcg injection
Site: Rotate sites
Inject within 30 minutes after training. Your body is primed for GH release and recovery during this window.
Before bed (30-60 minutes)
100-300 mcg injection
Site: Rotate sites
The bedtime dose synergizes with your natural overnight GH surge. Don't eat for at least 2 hours before this dose.
Dosing Tiers
Dose
100 mcg
Frequency
2-3 times daily
Duration
4-8 weeks
Start low to assess your body's response. Inject on an empty stomach for best absorption. Many people start with just twice daily (morning and before bed) to keep things simple.
Dose
200 mcg
Frequency
2-3 times daily
Duration
8-12 weeks
The sweet spot for most users. Injecting 200 mcg three times daily (morning, post-workout, and bedtime) maximizes GH pulses throughout the day while staying well-tolerated.
Dose
300 mcg
Frequency
3 times daily
Duration
8-12 weeks
Higher doses for those who've established tolerance. Some research used up to 1mg/kg, but most users find diminishing returns above 300 mcg per dose. Always combine with proper nutrition and training.
Preservation
Before Mixing
Keep your Ipamorelin powder in the refrigerator (36-46°F / 2-8°C) for regular storage. For long-term storage over 1 month, keep frozen at -4°F (-20°C). Store in original sealed vial away from light. Properly stored powder remains stable for 12-24 months.
After Mixing
Once mixed with bacteriostatic water, refrigerate at 36-46°F (2-8°C). Never freeze the reconstituted solution as this destroys the peptide. Keep away from light and use within 28-30 days for optimal potency.
Shelf Life After Mixing
28-30 days
Signs of Degradation
Discard the vial immediately if you notice any of these:
Cloudy or hazy appearance (should be crystal clear)
Visible particles floating or settled at the bottom
Color changes—any yellowing or discoloration means it's degraded
Reduced effectiveness after proper dosing (peptide may have broken down)
Important
When to Stop
Any signs of allergic reaction—stop immediately and seek medical help
Persistent carpal tunnel symptoms that worsen despite dose reduction
Significant joint pain that interferes with daily activities
Blood sugar control becomes significantly harder to manage
Unusual swelling that doesn't resolve with lower doses
Any side effect that feels serious or really concerns you
Ipamorelin is a research compound, not an FDA-approved medication. Never start, stop, or change your dosing without guidance from a qualified healthcare provider. This information is for educational purposes only—not medical advice.
Clean Technique Checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above