A next-generation growth hormone releaser that converts to hexarelin in your body for enhanced GH, cortisol, and unique aldosterone stimulation.
Written by Michael Carroll — Owner, Director of Research · Reviewed by the Peptide Initiative Research Team · Editorial standards
Subcutaneous
Route
4 recommended
Sites
Multiple times daily
Frequency
Preparation
Bacteriostatic water or normal saline (typically 1-2 mL per vial)
Sterile syringe and needle (25-27 gauge preferred)
Alcohol swabs for vial sterilization
Sterile vial for storage
Pro tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example calculation
If vial contains 1 mg alexamorelin and you add 1 mL bacteriostatic water, concentration = 1 mg/mL (1000 mcg/mL). To draw 100 mcg, inject 0.1 mL.
Dose calculation
Draw volume (mL) = Desired dose (mcg) ÷ Concentration (mcg/mL). For 150 mcg from 1000 mcg/mL solution: 150 ÷ 1000 = 0.15 mL
Pro tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 02
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Site 03
Lateral chest wall above waist
Follow your provider's instructions for this injection site. Rotate sites regularly to prevent tissue damage.
Site 04
Upper Arm
Back or outer area of the upper arm. This site may require assistance from another person for proper technique.
Rotate between 4 sites to prevent tissue buildup and ensure consistent absorption.
Pro tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by step
Clean injection site with alcohol swab and let dry 10-15 seconds
Pinch skin at injection site to create small fold
Insert needle at 45-90 degree angle (steeper = more subcutaneous, shallower = easier)
Push plunger slowly to minimize discomfort and ensure proper dispersion
Withdraw needle, release skin, and apply gentle pressure for 5 seconds
Pro tip
This peptide uses subcutaneous injection preferred for consistent gh response; intravenous only in clinical/research settings. Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal timing
Best time
Morning injection on empty stomach 30-60 minutes before food maximizes GH response; second dose in late afternoon if dosing twice daily
With food?
Separate from large meals by at least 30-60 minutes; food delays or blunts response. Light snack acceptable 2+ hours after injection
Stacking notes
If stacking with GHRH, inject GHRH 5-10 minutes after alexamorelin for synergistic effect. Avoid other GHS compounds on same day
Sample daily schedule
6:00-7:00 AM
100-150 mcg injection
Site: Lower abdomen
On empty stomach 30-60 minutes before breakfast. This is primary GH release window when natural cortisol is rising.
4:00-5:00 PM (optional second dose)
100-150 mcg injection
Site: Alternate side of abdomen or thigh
If dosing twice daily, inject 8+ hours after morning dose. Avoids consecutive injections at same site.
Evening (optional third dose for advanced users)
100 mcg injection
Site: Upper thigh or arm
Only if using advanced 3x daily protocol. Too late in day may interfere with sleep.
Dosing tiers
Dose
1.0 mcg/kg
Frequency
Single bolus per test session
Duration
Single-dose research study
Alexamorelin is a research growth-hormone-releasing peptide. In the main human study it was given as a single shot into a vein. The lower 1.0 mcg/kg dose raised growth hormone levels. [1]
Dose
2.0 mcg/kg
Frequency
Single bolus per test session
Duration
Single-dose research study
The higher IV dose used in the same human study produced a stronger growth hormone response. [1]
Preservation
Before mixing
Lyophilized powder at 2-8°C (refrigerator) in dark, dry location. Protect from light and moisture. Properly sealed vial lasts 1-2 years.
After mixing
Reconstituted solution at 2-8°C with bacteriostatic water. Use within 14-30 days. Discard if cloudy, discolored, or shows particles.
Shelf life after mixing
14-30 days when stored at 2-8°C with proper bacteriostatic agent; shorter if kept at room temperature
Signs of degradation
Discard the vial immediately if you notice any of these:
Solution becomes cloudy or milky instead of clear
Visible particles or crystallization in vial
Color change to yellow, brown, or any discoloration
Unusual odor or signs of bacterial contamination
Important
When to stop
Severe mood changes or anxiety that don't improve with dose adjustment
Signs of cardiovascular stress (chest pain, irregular heartbeat, severe headaches)
Uncontrolled blood sugar elevation or worsening of diabetes
Persistent allergic reactions or injection site infections
Completion of planned 4-6 week cycle (take break before restarting)
New or worsening joint problems that limit training
This is research compound information, not medical advice. Consult a healthcare provider before starting, especially if you have existing health conditions. Stop immediately if you experience serious side effects and seek medical attention.
Clean technique checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above
Published research
Broglio F, Benso A, Gottero C, et al. · 2000
In 6 young healthy subjects, alexamorelin showed identical GH-releasing potency to hexarelin at both IV doses (1.0 and 2.0 mcg/kg). Remarkably, alexamorelin demonstrated stronger ACTH-releasing activity than hexarelin and uniquely elevated aldosterone levels that hexarelin did not trigger. At oral 20mg dose, alexamorelin produced GH release comparable to or exceeding the same oral hexarelin dose.
Pobee E, Daziani G, Gameli PS · 2025
Alexamorelin undergoes rapid hepatic metabolism with approximately 150-fold signal decrease after just 3 hours in human hepatocyte studies. The primary metabolite formed is examorelin (hexarelin), created through C-terminal cleavage of the alanine amino acid by carboxypeptidase enzyme. This demonstrates alexamorelin functions as a prodrug that effectively converts to hexarelin within the body.
Ghigo E, Arvat E, Camanni F · 1998
Comprehensive review of GHS compounds including alexamorelin's class showing potent, dose-dependent, and reproducible GH-releasing effects across IV, subcutaneous, intranasal, and oral administration routes. GHS molecules activate the GHS receptor (ghrelin receptor) and synergize with GHRH for enhanced effects. Prolonged intermittent oral administration increases IGF-1 levels. The class shows clinical promise for GH deficiency, aging-related issues, critical illness, and muscle wasting conditions.
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