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Injection Guide
The only FDA-approved peptide specifically designed to melt away stubborn belly fat by telling your brain to release more growth hormone naturally—working with your body's own systems rather than replacing them.
Route
Subcutaneous
Sites
3 recommended
Frequency
Once daily
Preparation
Tesamorelin powder vial
Sterile water for injection (provided with prescription products)
Insulin syringes (29-31 gauge, 1/2 inch needle)
Alcohol swabs
Sharps disposal container
Pro Tip
Prepare all supplies on a clean surface before you begin. Having everything ready makes the process smoother and more sterile.
Mixing
Wash your hands thoroughly with soap and water. Gather all supplies on a clean, flat surface.
Remove the plastic cap from the peptide vial and wipe the rubber stopper with an alcohol swab. Let it air dry.
Draw the appropriate amount of bacteriostatic water into a sterile syringe.
Insert the needle into the vial at an angle, aiming at the inside wall of the vial. Slowly push the plunger to let the water trickle down the glass wall -- do NOT squirt directly onto the powder.
Once all water is added, gently swirl the vial in a slow circular motion. Never shake the vial, as this can damage the peptide bonds.
Continue swirling until the powder is completely dissolved and the solution is clear. If particles remain, let the vial sit for a few minutes and swirl again.
Label the vial with the date of reconstitution, the peptide name, and the concentration (e.g. 250mcg per 0.1mL).
Example Calculation
For Egrifta SV (2mg vial): Add the entire contents of the provided sterile water diluent (0.5 mL) to the vial. This gives you a concentration of 4 mg/mL. The prescribed dose of 1.4 mg = 0.35 mL to inject.
Dose Calculation
At 4 mg/mL concentration: 1.4 mg dose = 0.35 mL (35 units on an insulin syringe). Always follow the specific reconstitution instructions for your particular product, as formulations differ.
Pro Tip
Always add the bacteriostatic water slowly, letting it run down the side of the vial. Never shake the vial -- swirl gently to avoid damaging the peptide.
Location
Site 01
Abdomen
Pinch the skin 2 inches from navel. Avoid the area directly around the belly button. Rotate between left and right sides.
Site 02
Outer Thigh
Middle third of the outer thigh. Keep at least 4 inches above the knee and below the hip. Alternate legs each injection.
Site 03
Rotate sites to prevent lipodystrophy at injection locations
Follow your provider's instructions for this injection site. Rotate sites regularly to prevent tissue damage.
Rotate between 3 sites to prevent tissue buildup and ensure consistent absorption.
Pro Tip
Rotate your injection sites with each dose to prevent lipohypertrophy (buildup of fatty tissue). Keep a simple log of where you last injected.
Step by Step
Wash hands thoroughly with soap and water
Clean the injection site with an alcohol swab and let dry completely
Pinch a fold of skin between thumb and forefinger
Insert needle at a 45 to 90-degree angle (90 for more tissue, 45 for lean individuals)
Inject slowly and steadily over 5-10 seconds
Wait 5 seconds before withdrawing the needle
Do not rub the injection site afterward
Pro Tip
This peptide uses subcutaneous injection (just under the skin into fatty tissue)—this is the only approved administration route. Inject at a 45-90 degree angle into pinched skin. Aspirate before injecting to ensure you haven't hit a blood vessel.
Timing
Optimal Timing
Best time
Inject in the morning on an empty stomach, at least 30 minutes before eating. This aligns with your natural cortisol awakening response and may optimize growth hormone release patterns.
With food?
Do NOT inject with food. Tesamorelin should be given on an empty stomach for optimal absorption. Wait at least 30 minutes after injection before eating breakfast.
Stacking notes
If using with other growth hormone secretagogues, separate injections by several hours. Some practitioners alternate between tesamorelin and other GHRH peptides rather than using them the same day. Never combine with exogenous GH—choose one or the other.
Sample Daily Schedule
Morning (before breakfast)
1.4-2 mg depending on formulation injection
Site: Rotate between left and right abdomen
Inject on an empty stomach at least 30 minutes before eating. Consistency is key—try to inject at the same time each day. Morning dosing may align better with natural GH release patterns.
Dosing Tiers
Dose
1 mg
Frequency
Once daily
Duration
2-4 weeks
Some practitioners start at the lower approved dose to assess tolerance before increasing. The standard FDA-approved dose is 2mg, but starting lower allows you to monitor for side effects like injection site reactions or joint discomfort.
Dose
2 mg (or 1.4 mg Egrifta SV)
Frequency
Once daily
Duration
26 weeks or longer
This is the FDA-approved dose shown effective in large Phase 3 trials. Clinical studies showed significant visceral fat reduction at 26 weeks with continued benefits at 52 weeks. Note: Egrifta SV uses a 1.4mg dose which is bioequivalent to the original 2mg formulation.
Dose
2 mg
Frequency
Once daily
Duration
52+ weeks
Long-term use beyond the initial 26 weeks has been studied and shows maintained benefits. However, stopping treatment causes visceral fat to return. Many users continue indefinitely with periodic monitoring. Extended use requires regular IGF-1 and glucose monitoring.
Preservation
Before Mixing
Store powder in refrigerator at 36-46°F (2-8°C). Keep in original carton to protect from light. Do not freeze. Check expiration date on package—stable until that date if properly stored.
After Mixing
Refrigerate reconstituted solution at 36-46°F (2-8°C). Use within 14 days for Egrifta SV (original Egrifta had shorter stability). Never freeze reconstituted solution. If solution becomes cloudy or contains particles, do not use.
Shelf Life After Mixing
14 days (Egrifta SV) to 24-48 hours (original formulation)
Signs of Degradation
Discard the vial immediately if you notice any of these:
Cloudy or hazy appearance (should be clear and colorless)
Visible particles or floaters
Any color change from clear/colorless
Solution that doesn't dissolve completely during reconstitution
Important
When to Stop
Severe allergic reaction occurs—stop immediately and seek emergency care
Visceral fat has not decreased after 3 months of treatment (per FDA guidance)
Blood glucose becomes uncontrolled despite adjustments
Development of new or worsening malignancy
Pregnancy occurs or is planned
Intolerable side effects that don't resolve with dose adjustment
Tesamorelin is an FDA-approved prescription medication that should only be used under physician supervision. Never start, stop, or adjust your dose without consulting your prescribing healthcare provider. This information is educational and does not constitute medical advice.
Clean Technique Checklist
Wash hands thoroughly with soap and water before handling supplies
Swab vial tops and injection site with alcohol and let dry
Never touch the needle tip or allow it to contact non-sterile surfaces
Use a new syringe and needle for each injection
Dispose of used sharps in a proper sharps container
Store reconstituted peptides according to the storage instructions above