Healing & Recovery Protocol
Thymosin Beta-4Complete Dosing & Administration Guide
The body's natural healing peptide that accelerates tissue repair and reduces inflammation
Dose Range
2-5mg
Frequency
Twice weekly
Route
Subcutaneous injection
Cycle Length
8-12 weeks
Dosing
How much
do I take?
Timing
Best time to take
Morning or evening (flexible)
With food?
Not affected by food intake
If stacking
Best administered on different days than other injectable peptides to minimize injection site stress
Adjusting Your Dose
Increase if
- +Healing is slower than expected after 3-4 weeks
- +Inflammation remains high despite initial treatment
- +Underlying injury is more severe than initially assessed
Decrease if
- -Injection site reactions become bothersome
- -Excessive bruising or swelling develops
- -Systemic sensitivity symptoms appear
Signs of right dose
- ✓Visible improvement in wound appearance by week 2-3
- ✓Reduced pain and inflammation
- ✓Improved range of motion around injured area
- ✓Faster scar maturation
Dosing Calculator
Calculate Your Exact Dose
Step 1: Peptide Weight
Find the weight printed on your peptide vial label
Look here!
The peptide weight is printed on the label
Look here!
The weight is on the label
Administration
How do I
use it?
Reconstitution
What you need
- •Bacteriostatic water or normal saline
- •Sterile syringe (3-5 mL recommended)
- •Sterile needle (25-27 gauge)
- •Alcohol swabs
- •Vial of Thymosin Beta-4 powder
Injection
Route
Subcutaneous
Best sites
- •Lower abdomen (spare 2 inches around navel)
- •Outer thigh
- •Upper arm (back of triceps)
- •Rotate sites with each injection
Technique
- 1.Clean skin with alcohol swab and let dry
- 2.Pinch skin gently to create subcutaneous fold
- 3.Insert needle at 45-90 degree angle
- 4.Inject slowly over 2-3 seconds
- 5.Withdraw needle and apply light pressure
- 6.Rotate injection sites to prevent lipohypertrophy
Storage
Signs of degradation
Sample Daily Schedule
Safety
Is it
safe?
Safety Profile
Thymosin Beta-4 is a naturally occurring peptide found in all cells and body fluids in the human body. Clinical trials have demonstrated excellent safety even at very high doses. IV studies showed doses up to 1260 mg were well tolerated with no serious adverse events.
Multiple Phase 1, Phase 2, and Phase 3 clinical trials completed. Phase 1 study showed dose-proportional pharmacokinetics with increasing half-life at higher doses, indicating predictable and safe dosing.
Common Side Effects
Experienced by some users
Injection site redness
Mild redness at the site of injection, usually resolves within hours to a few days
Management: Ice pack immediately after injection, rotate injection sites, use proper sterile technique
Injection site swelling
Mild swelling or puffiness at injection site, typically mild and temporary
Management: Ice compress for 10-15 minutes after injection, elevate area if applicable
Less Common
- •Temporary mild headache
- •Mild bruising at injection site
- •Transient fatigue
These typically resolve with continued use or dose adjustment.
Stop and Seek Help If
- ×Severe allergic reaction or anaphylaxis
- ×Persistent injection site infection
- ×Significant systemic adverse effects
- ×Pregnancy is confirmed
- ×No improvement in healing after 12 weeks (reassess approach)
- ×Active systemic infection develops without antibiotic coverage
This is general educational information. Always consult with a healthcare provider before starting, stopping, or modifying any peptide therapy. Do not self-diagnose or treat serious medical conditions.
Interactions
With other peptides
- !
- !
- !
- !
With medications
- !
- !
- !
- !
With supplements
- ✓
- ✓
- ✓
- ✓
- ✓
Want the Full Picture?
View the complete Thymosin Beta-4 research profile including mechanism of action, clinical studies, effectiveness timeline, and FAQ.
View Full Thymosin Beta-4 Profile