Anti-Aging Protocol
LivagenComplete Dosing & Administration Guide
Tetrapeptide bioregulator (Lys-Glu-Asp-Ala) that directly penetrates cell nuclei and binds double-stranded DNA to induce chromatin decondensation in senescent lymphocytes, activate ribosomal genes, inhibit enkephalin-degrading enzymes with an IC50 of 20 μM, and restore protein synthesis capacity in aging cells through selective epigenetic remodeling
Dose Range
2-10mg
Frequency
Once daily
Route
Intramuscular injection
Cycle Length
8-12 weeks
Dosing
How much
do I take?
Starting Dose
2 mg subcutaneously once daily
Conservative research protocol. Reconstitute lyophilized Livagen in bacteriostatic water. Administer subcutaneously, rotating injection sites. Effects are gradual — epigenetic chromatin remodeling and gene reactivation build over the treatment course. Monitor for injection site reactions. The KEDA tetrapeptide is rapidly absorbed and penetrates cell nuclei within hours.
Standard Dose
5-10 mg intramuscularly once daily
Standard Khavinson laboratory protocol delivering a total course dose of 50-100 mg over 10 days. Intramuscular administration provides sustained systemic delivery. Can also be administered subcutaneously. Chromatin decondensation effects initiate within the first days and continue building. Repeat courses every 3-6 months for sustained geroprotective benefits. Can be combined with Epithalon for comprehensive anti-aging support.
Advanced Dose
10 mg intramuscularly once daily
Extended protocol for individuals with significant age-related decline in liver function or immune competence. Oral capsule form (0.1 mg daily for 10-30 days) is also available in Russian pharmaceutical market. Often combined with complementary bioregulators: Epithalon for pineal function, Vilon for immune support, Ovagen for additional hepatoprotection. Medical supervision recommended.
Timing
Best time to take
Administer Livagen at the same time each day (or on the same day each week for weekly injections). Many users prefer morning or evening administration. Pick a time you'll remember consistently.
With food?
Livagen injections can be given regardless of meal timing. However, if GI effects occur, administering on an empty stomach or with a light meal may help reduce discomfort.
If stacking
Livagen should be used as directed by your healthcare provider. If combining with other medications or supplements, discuss potential interactions with your provider. Avoid combining with compounds that have overlapping mechanisms unless specifically guided by a medical professional.
Adjusting Your Dose
Increase if
- +You've tolerated the current dose for the recommended period without significant side effects
- +Therapeutic goals haven't been met at the current dose level
- +Your healthcare provider recommends dose escalation based on your response
- +Lab work or clinical assessments support a higher dose
Decrease if
- -Side effects are bothersome or impacting daily life despite management strategies
- -You experience any signs of an adverse reaction
- -Lab results indicate the need for dose reduction
- -Your healthcare provider recommends a lower dose based on your response
Signs of right dose
- ✓Therapeutic goals being met with minimal side effects
- ✓Stable and consistent response to treatment
- ✓Lab values or clinical markers trending in the right direction
- ✓Good tolerance with manageable or absent side effects
Dosing Calculator
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Administration
How do I
use it?
Reconstitution
What you need
- •Livagen vial (lyophilized powder or solution)
- •Bacteriostatic water or sterile sodium chloride for reconstitution
- •Alcohol swabs for cleaning vial tops and injection sites
- •Appropriately sized syringes with fine-gauge needles (27-30 gauge)
- •Sharps disposal container
Injection
Route
Subcutaneous injection (into the fatty tissue just under the skin)—allows for consistent absorption and can be self-administered at home after proper training
Best sites
- •Abdomen (stomach area)—at least 2 inches from the belly button, most popular choice for self-injection
- •Front of thighs—middle to upper portion of the outer leg
- •Back of upper arm—outer area (may need assistance from another person)
Technique
- 1.Wash your hands thoroughly with soap and water before handling supplies
- 2.Clean the injection site with an alcohol swab and let it air dry completely
- 3.Pinch a fold of skin at the chosen injection site
- 4.Insert the needle at a 45-90 degree angle (depending on needle length and body composition)
- 5.Inject the medication slowly and steadily over 5-10 seconds
- 6.Release the skin fold and remove the needle, applying gentle pressure with a clean swab
- 7.Rotate injection sites to prevent tissue irritation or lipodystrophy
- 8.Dispose of the needle safely in a sharps container—never recap or reuse needles
Storage
Signs of degradation
Sample Daily Schedule
Safety
Is it
safe?
Safety Profile
Livagen (a liver-derived peptide extract) carries a safety profile established through decades of use in Eastern European hepatology and gastroenterology clinics. As an organ-derived extract rather than a synthetic single peptide, it contains multiple bioactive components promoting hepatocyte regeneration and cytoprotection. Adverse events are minimal in reported clinical experience—mild gastrointestinal upset occurring in <5% of patients. The compound shows no hepatotoxicity, nephrotoxicity, or immunosensitization in available safety surveillance data from thousands of patients.
Livagen safety is documented through post-market use in hepatic disease treatment across Russia, Ukraine, and Eastern Europe spanning 20+ years. Russian-language clinical trials demonstrate tolerability in chronic hepatitis and cirrhosis without serious adverse events. Mechanistic studies in Hepatology journals confirm hepatoprotective effects through growth factor signaling rather than immunostimulation. Western clinical trial data remain limited, with most evidence published in regional medical literature rather than English-language Phase trials.
Common Side Effects
Experienced by some users
Injection site reaction
Mild redness, slight soreness, or minor swelling at the intramuscular or subcutaneous injection site. Most frequently reported side effect across clinical studies.
Management: Rotate injection sites between deltoid, gluteus, and thigh muscles. Apply cold compress if needed. Typically resolves within 24-48 hours.
Mild fatigue
Transient fatigue during the initial days as epigenetic chromatin remodeling activates previously silenced gene programs and protein synthesis recalibrates.
Management: Rest as needed. Usually resolves within 2-3 days. Ensure adequate sleep and hydration.
Mild headache
Occasional headache during the first few days, potentially related to enkephalinase inhibition altering endogenous opioid peptide dynamics.
Management: Ensure adequate hydration. Standard analgesics if needed. Self-limiting within 1-2 days.
Digestive enzyme changes
Mild gastrointestinal adjustments reflecting Livagen's enhancement of digestive enzyme activity (invertase, maltase, aminopeptidases) throughout the GI tract.
Management: Generally benign — indicates active enzyme normalization. Eat regular meals. Should stabilize within days.
Less Common
- •Transient flu-like symptoms
These typically resolve with continued use or dose adjustment.
Stop and Seek Help If
- ×Severe or worsening side effects that don't improve with dose adjustment or supportive care
- ×Signs of an allergic reaction—rash, hives, swelling, or difficulty breathing
- ×Your healthcare provider recommends discontinuation based on your clinical response
- ×Development of any new medical condition that may be contraindicated with Livagen
- ×Pregnancy or planning to become pregnant (unless specifically approved for use during pregnancy)
- ×Abnormal lab results or clinical markers that suggest adverse effects
Livagen should only be started, adjusted, or discontinued under medical supervision. This information is for educational purposes only and does not replace professional medical advice. Never stop a prescribed treatment without consulting your healthcare provider first, as abrupt discontinuation may have consequences.
Interactions
With other peptides
- ✓May be used together under medical guidance.
- ✓May be used together under medical guidance.
- ✓May be used together under medical guidance.
With medications
- !Opioid medications (Livagen inhibits enkephalinase with IC50 of 20 μM, potentially prolonging endogenous opioid activity) - Use with caution—discuss with your healthcare provider.
- !Immunosuppressive drugs without medical supervision — chromatin reactivation may enhance immune gene expression - Use with caution—discuss with your healthcare provider.
- !Other potent peptidase inhibitors — potential for additive effects on enzyme inhibition - Use with caution—discuss with your healthcare provider.
With supplements
- ✓Multivitamins - Generally safe to take alongside Livagen. Space doses apart if taking oral formulations to ensure optimal absorption.
- ✓Electrolyte supplements - Helpful if experiencing any GI side effects that could lead to dehydration. Safe to combine.
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