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Peptide Database

Goals
Fat LossMuscle BuildingInjury HealingAnti-AgingCognitive EnhancementSleep OptimizationImmune SupportGut HealingSkin RejuvenationSexual Health
Peptides
Adipotide
Weight Management
AOD-9604
Weight Management
BPC-157
Healing & Recovery
Cagrilintide
Weight Management
CJC-1295
Growth Hormone
DSIP
Sleep & Recovery
Epithalon
Anti-Aging
GHK-Cu
Anti-Aging
GHRP-2
Growth Hormone
HCG
Hormone Support
Hexarelin
Growth Hormone
HGH
Growth Hormone
IGF-1 LR3
Growth Hormone
Kisspeptin
Hormone Support
Melanotan-2
Cosmetic
MOTS-C
Metabolic
NAD+
Anti-Aging
Oxytocin Acetate
Hormone Support
PEG-MGF
Recovery
PNC-27
Cancer Research
PT-141
Sexual Health
Retatrutide
Weight Management
Selank
Cognitive
Semaglutide
Weight Management
Semax
Cognitive
Sermorelin
Growth Hormone
Snap-8
Cosmetic
SS-31
Mitochondrial
TB-500
Healing & Recovery
Tesamorelin
Growth Hormone
Thymosin Alpha-1
Immune
Tirzepatide
Weight Management
Total Peptides: 32
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Anti-Aging Protocol

EpithalonComplete Dosing & Administration Guide

A tiny but mighty four-amino-acid peptide that activates your body's telomerase enzyme—essentially helping your cells hit the 'reset' button on aging by protecting and lengthening the protective caps on your chromosomes.

Dose Range

5-10mg

Frequency

Once daily

Route

Subcutaneous injection

Cycle Length

4-6 weeks

Dosing

How much
do I take?

Starting Dose

5 mg

Frequency

Once daily

Duration

10 days

This is the classic Russian protocol starting point. Inject once daily for 10 consecutive days, then take a 3-6 month break before your next cycle. Think of it as a 'cellular tune-up' you do a couple times per year.

Standard Dose

10 mg

Frequency

Once daily

Duration

10-20 days

The most commonly used protocol in research settings. Some people split this into two cycles per year (10 days each) or do one longer 20-day cycle annually. Both approaches have been studied.

Advanced Dose

10 mg

Frequency

Once daily

Duration

20-30 days

Extended protocols for those with established tolerance. Some research has used up to 30 consecutive days. Longer doesn't always mean better—the key is consistency over years, not cramming in more per cycle.

Timing

Best time to take

Evening injections (1-2 hours before bed) work best for most people since Epithalon supports melatonin production—aligning with your natural sleep cycle makes sense.

With food?

Epithalon can be taken regardless of food timing. It doesn't seem to affect absorption either way, so fit it into whatever routine works for you.

If stacking

If stacking with other peptides, inject them at different sites. Epithalon pairs nicely with GHK-Cu for a comprehensive anti-aging approach. Space injections at least 30 minutes apart to track individual effects.

Adjusting Your Dose

Increase if

  • +You've completed the starting protocol with zero issues
  • +Your research goals require extended treatment periods
  • +You're following a protocol that calls for higher doses under supervision

Decrease if

  • -You experience persistent headaches beyond the first few days
  • -Injection site reactions don't resolve within 48 hours
  • -Sleep quality worsens instead of improves
  • -Any unexpected or uncomfortable side effects arise

Signs of right dose

  • Improved sleep quality and more consistent sleep patterns
  • Stable energy levels throughout the day
  • No injection site issues beyond initial redness
  • General sense of well-being maintained or improved

Dosing Calculator

Calculate Your Exact Dose

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Step 1: Peptide Weight

Find the weight printed on your peptide vial label

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The weight is on the label

Peptide vial
5mg

Select Weight

mg

Look for a number followed by 'mg' on the vial label (e.g., 5mg, 10mg)

Administration

How do I
use it?

Reconstitution

What you need

  • Bacteriostatic water (BAC water)—the preservative keeps it safe for multiple uses
  • Insulin syringes (29-31 gauge)—thin needles mean less discomfort
  • Alcohol swabs for cleaning vial tops and injection sites
  • Your Epithalon powder vial

Example

If you have a 10mg vial and add 2mL of BAC water, you get a concentration of 5mg/mL. So every 0.2mL (20 units on an insulin syringe) equals 1mg of Epithalon.

For a 5mg dose at 5mg/mL concentration: draw 1mL (100 units on a standard insulin syringe). For a 10mg dose: draw the full 2mL or use two separate syringes.

Injection

Route

Subcutaneous injection (just under the skin)—the easiest and most common method for self-administration

Best sites

  • Belly fat area (about 2 inches away from your belly button)
  • Front or outer thigh (middle section)
  • Back of the upper arm (have someone help if needed)

Technique

  • 1.Wash your hands thoroughly with soap and water
  • 2.Clean the injection site with an alcohol swab and let it air dry completely
  • 3.Pinch about an inch of skin to create a fold
  • 4.Insert the needle at a 45-90 degree angle (45 if you're lean, 90 if you have more tissue)
  • 5.Push the plunger slowly and steadily
  • 6.Wait 5 seconds before removing the needle
  • 7.Apply light pressure if needed—don't rub
Full Injection Guide

Storage

Before reconstitution

Keep your Epithalon powder in the refrigerator (36-46°F / 2-8°C) for short-term storage or in the freezer (-4°F / -20°C) for long-term storage. Store in the original sealed vial away from light. Properly stored powder can remain stable for 2+ years when frozen.

After reconstitution

Once mixed with bacteriostatic water, refrigerate at 36-46°F (2-8°C). Never freeze the reconstituted solution—freezing destroys the peptide. Keep away from light and use within 21-28 days.

Signs of degradation

  • Cloudy or hazy appearance (should be crystal clear)
  • Visible particles floating or settled at the bottom
  • Color changes—any yellowing or discoloration means toss it
  • Unusual smell—fresh solution should have little to no odor

Sample Daily Schedule

Evening (7-9 PM, about 1-2 hours before bed)

5-10 mg depending on protocol injection

Site: Rotate between belly, thigh, and arm

Evening timing aligns with your body's natural melatonin production cycle. Follow a 10-20 day cycle, then take 3-6 months off before repeating. Most research uses 1-2 cycles per year.

Safety

Is it
safe?

Safety Profile

Epithalon has shown a favorable safety profile across decades of research, primarily conducted in Russia. Studies spanning up to 15 years of follow-up have reported no severe adverse events in older adults using Epithalon or its natural precursor Epithalamin. The peptide appears well-tolerated with minimal side effects, mostly limited to mild injection site reactions. However, large-scale clinical trials outside of Russia are still needed.

Most safety data comes from Russian research conducted by Dr. Khavinson's team at the St. Petersburg Institute of Bioregulation and Gerontology. While multiple studies suggest a good safety profile, independent replication and Western clinical trials remain limited. Always work with a healthcare provider when using research compounds.

Common Side Effects

Experienced by some users

Injection site redness

A little pink or red area where you injected is totally normal. It's just your skin reacting to the needle and solution.

Management: Rotate injection sites between belly, thigh, and arm. A cool compress can help. Usually fades within 24-48 hours.

Mild headache

Some users report mild headaches in the first few days, possibly related to changes in melatonin or pineal gland activity.

Management: Stay well-hydrated and rest if needed. These typically resolve within the first week as your body adjusts.

Temporary fatigue

Transient fatigue or mild tiredness occurs in approximately 10-15% of Epithalon users, typically in the first 3-5 days of a cycle. Fatigue is usually mild to moderate, lasting 2-4 hours after injection, and characteristically resolves within the first week as the body adjusts. The mechanism is unclear but may relate to pineal gland reactivation, melatonin upregulation, or circadian rhythm recalibration. Fatigue is more common with evening injections and less common when Epithalon is dosed in the morning.

Management: Injecting in the evening (1-2 hours before bed) is optimal since you'll sleep through any fatigue, which typically improves after day 3-4. If fatigue significantly impacts daytime function, consider morning injection instead. Ensure adequate hydration and consistent sleep schedule. Fatigue on repeat cycles (second and subsequent rounds) usually improves or disappears entirely, suggesting the adjustment is temporary.

Less Common

  • Vivid dreams
  • Sleep pattern changes

These typically resolve with continued use or dose adjustment.

Stop and Seek Help If

  • ×Any signs of allergic reaction—stop immediately and seek medical help
  • ×Persistent headaches that don't improve after the first week
  • ×Significant sleep disturbances that worsen rather than improve
  • ×Injection site reactions that spread or don't heal
  • ×Any side effect that feels serious or really concerns you
  • ×Your research protocol/cycle is complete

Epithalon is a research compound, not an FDA-approved medication. Never start, stop, or change your dosing without guidance from a qualified healthcare provider. This information is for educational purposes only—not medical advice.

Interactions

With other peptides

  • Excellent pairing for comprehensive anti-aging support. GHK-Cu works on skin and tissue repair while Epithalon works at the cellular/telomere level. Inject at different sites.
  • Both support immune function through different mechanisms. Can be used together in anti-aging protocols—they're actually from the same research group.
  • Safe to combine. BPC-157 focuses on healing and gut health, complementing Epithalon's cellular anti-aging effects.
  • Both affect sleep. While not necessarily dangerous, using both may cause excessive drowsiness. Use caution and monitor your response.

With medications

  • !Immunosuppressants (cyclosporine, tacrolimus) - Epithalon supports immune function, which could potentially counteract immunosuppressive therapy. Consult your doctor.
  • !Sleep medications (Ambien, benzodiazepines) - Epithalon increases melatonin production, which could compound sedative effects. Use caution and inform your doctor.
  • Blood thinners (warfarin, aspirin) - May increase bruising at injection sites. Not dangerous but worth monitoring. Inform your doctor.
  • Diabetes medications - Some peptides affect glucose metabolism. Monitor blood sugar more frequently during Epithalon cycles.

With supplements

  • Melatonin - Since Epithalon boosts natural melatonin production, adding supplemental melatonin may cause excessive drowsiness. Reduce or eliminate melatonin supplements during cycles.
  • Vitamin D - Safe and potentially complementary. Vitamin D supports immune function and overall health.
  • Zinc - Safe to take together. Zinc supports immune function and general cellular health.
  • High-dose antioxidants - Theoretical concern: megadoses of vitamin C or E might interfere with Epithalon's mechanisms. Stick to normal supplement doses.

Want the Full Picture?

View the complete Epithalon research profile including mechanism of action, clinical studies, effectiveness timeline, and FAQ.

View Full Epithalon Profile

Medical Disclaimer

Epithalon is an investigational research compound not approved by the FDA for human therapeutic use. This information is for educational purposes only and should not be construed as medical advice. Always consult with a qualified healthcare provider before starting any new supplement or treatment protocol.

Last updated: 2/8/2026

Epithalon Protocol — Complete Dosing & Administration Guide | Peptide Initiative