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Peptides
Adipotide
Weight Management
AOD-9604
Weight Management
BPC-157
Healing & Recovery
Cagrilintide
Weight Management
CJC-1295
Growth Hormone
DSIP
Sleep & Recovery
Epithalon
Anti-Aging
GHK-Cu
Anti-Aging
GHRP-2
Growth Hormone
HCG
Hormone Support
Hexarelin
Growth Hormone
HGH
Growth Hormone
IGF-1 LR3
Growth Hormone
Kisspeptin
Hormone Support
Melanotan-2
Cosmetic
MOTS-C
Metabolic
NAD+
Anti-Aging
Oxytocin Acetate
Hormone Support
PEG-MGF
Recovery
PNC-27
Cancer Research
PT-141
Sexual Health
Retatrutide
Weight Management
Selank
Cognitive
Semaglutide
Weight Management
Semax
Cognitive
Sermorelin
Growth Hormone
Snap-8
Cosmetic
SS-31
Mitochondrial
TB-500
Healing & Recovery
Tesamorelin
Growth Hormone
Thymosin Alpha-1
Immune
Tirzepatide
Weight Management
Total Peptides: 32
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Sleep & Recovery Protocol

DSIP (Delta Sleep-Inducing Peptide)Complete Dosing & Administration Guide

A naturally occurring brain peptide that helps you fall into deep, restorative sleep by gently nudging your body's own sleep-wake systems—without the groggy hangover of traditional sleep aids.

Dose Range

100-300mcg

Frequency

Once daily

Route

Subcutaneous injection

Cycle Length

4-6 weeks

Dosing

How much
do I take?

Starting Dose

100 mcg

Frequency

Once daily, 1-2 hours before bed

Duration

1-2 weeks

Start here to see how your body responds. Many people notice improved sleep quality within the first few nights. This gentle introduction helps you gauge your sensitivity before moving up.

Standard Dose

200 mcg

Frequency

Once daily, 1-2 hours before bed

Duration

4-6 weeks

The most commonly used dose for consistent sleep improvement. Research studies typically used similar amounts and found good results without significant side effects. This is where most people find their sweet spot.

Advanced Dose

300 mcg

Frequency

Once daily, 1-2 hours before bed

Duration

2-4 weeks

The higher end of typical dosing. Some people with severe sleep disruption or high stress levels may benefit from this dose. Always work with a healthcare provider at this level.

Timing

Best time to take

Evening, 1-2 hours before your intended bedtime. DSIP doesn't knock you out instantly—it works with your body's natural sleep systems, so give it time to take effect.

With food?

Can be taken with or without food. Some people prefer taking it on an empty stomach for potentially faster absorption, but food doesn't block its effects.

If stacking

If using with other peptides like CJC-1295 or Ipamorelin for growth hormone support, inject at different sites. DSIP is typically taken alone in the evening while growth hormone peptides may be used at other times.

Adjusting Your Dose

Increase if

  • +You've been at the current dose for 2+ weeks with minimal improvement
  • +Sleep quality improved initially but has plateaued
  • +No side effects at current dose and you want better results
  • +Your healthcare provider recommends escalation

Decrease if

  • -You experience morning grogginess or headaches
  • -Sleep becomes too deep and you have trouble waking to alarms
  • -Vivid dreams become disturbing rather than pleasant
  • -Any uncomfortable side effects persist

Signs of right dose

  • Falling asleep within 20-30 minutes of lying down
  • Sleeping through the night with minimal waking
  • Waking feeling refreshed without an alarm
  • Improved daytime energy and mental clarity

Dosing Calculator

Calculate Your Exact Dose

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Step 1: Peptide Weight

Find the weight printed on your peptide vial label

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The weight is on the label

Peptide vial
5mg

Select Weight

mg

Look for a number followed by 'mg' on the vial label (e.g., 5mg, 10mg)

Administration

How do I
use it?

Reconstitution

What you need

  • Bacteriostatic water (BAC water)
  • Insulin syringes (29-31 gauge)
  • Alcohol swabs
  • Your DSIP powder vial (typically 2mg or 5mg)

Example

For a 2mg vial: Add 2mL of bacteriostatic water. This gives you 1mg/mL (1000 mcg/mL). For a 200mcg dose, you'll draw 0.2mL (20 units on an insulin syringe).

At 1000 mcg/mL concentration: 100mcg = 0.1mL (10 units), 200mcg = 0.2mL (20 units), 300mcg = 0.3mL (30 units). Always double-check your math!

Injection

Route

Subcutaneous injection (just under the skin)—simple and effective for self-administration

Best sites

  • Abdomen (2 inches from navel)—easy access and good absorption
  • Front of thigh—convenient alternative
  • Back of upper arm—may need assistance

Technique

  • 1.Wash hands thoroughly with soap and water
  • 2.Clean injection site with alcohol swab and let it dry completely
  • 3.Pinch a fold of skin between thumb and forefinger
  • 4.Insert needle at 45-90 degree angle (45° if lean, 90° if more tissue)
  • 5.Inject slowly and smoothly over 5-10 seconds
  • 6.Wait a moment, then withdraw needle and apply light pressure—don't rub
Full Injection Guide

Storage

Before reconstitution

Keep your DSIP powder in the freezer (-20°C/-4°F or colder). Store in its original sealed vial, protected from light. A properly stored vial can remain stable for up to 24 months. Never leave powder at room temperature for extended periods.

After reconstitution

Once mixed with bacteriostatic water, immediately refrigerate at 36-46°F (2-8°C). Keep the vial upright and away from light. Use within 28 days. Never freeze the reconstituted solution—this destroys the peptide.

Signs of degradation

  • Cloudy or murky solution (should be clear)
  • Visible particles or floaters
  • Any unusual color changes
  • Unusual smell (fresh solution has minimal odor)

Sample Daily Schedule

Evening (1-2 hours before bedtime)

100-300 mcg based on your tier injection

Site: Abdomen or thigh—rotate sites

Take at a consistent time each evening for best results. DSIP works with your natural sleep systems, so timing matters. Aim for the same window each night, ideally 1-2 hours before you want to be asleep. A 4-6 week cycle is typical, followed by a break to assess your sleep without it.

Safety

Is it
safe?

Safety Profile

DSIP has an excellent safety record across decades of research. In human studies, it was described as 'incredibly safe' with no lethal dose ever identified in animal studies. Side effects are generally mild and temporary—mostly headache, brief dizziness, or flushing. The peptide works by supporting natural sleep processes rather than forcing sedation, which is why it doesn't cause the dependency or withdrawal issues seen with pharmaceutical sleep aids.

DSIP has been studied in humans since the early 1980s, with multiple double-blind placebo-controlled trials. While not as extensively studied as FDA-approved medications, the available evidence shows consistent safety with meaningful benefits for sleep. Most research focused on short-to-medium term use (days to weeks).

Common Side Effects

Experienced by some users

Mild headache

The most frequently reported side effect. Usually appears within an hour of injection and resolves on its own.

Management: Stay well-hydrated. If persistent, try reducing your dose. Over-the-counter pain relievers can help if needed, but usually aren't necessary.

Brief dizziness or lightheadedness

Some people feel slightly lightheaded shortly after injection. This is usually mild and passes within 15-30 minutes.

Management: Sit or lie down after injecting until it passes. Make sure you're not injecting on an empty stomach if this bothers you.

Flushing or warmth

A temporary sensation of warmth, sometimes with mild skin flushing. Completely harmless and usually brief.

Management: This is normal and passes on its own. Stay in a comfortable environment and relax—you're about to sleep anyway!

Injection site reactions

Minor redness, tenderness, or slight swelling where you injected. Very common with any injectable.

Management: Rotate injection sites to give each area time to recover. Ensure proper injection technique. Cool compress can help if bothersome.

Less Common

  • Vivid dreams
  • Mild nausea

These typically resolve with continued use or dose adjustment.

Stop and Seek Help If

  • ×Any signs of allergic reaction—hives, swelling, breathing difficulty (stop immediately, seek help)
  • ×Severe or persistent headaches that don't respond to dose reduction
  • ×Excessive daytime drowsiness affecting your ability to function
  • ×Unusual mood changes or psychological symptoms
  • ×You've completed your planned cycle (typically 4-6 weeks)
  • ×Your healthcare provider recommends discontinuation

DSIP is a research peptide, not an FDA-approved medication. Always work with a qualified healthcare provider when using peptides. Never start, stop, or change doses without professional guidance. This information is educational—not medical advice.

Interactions

With other peptides

  • Complementary pairing—Epitalon supports melatonin production while DSIP enhances delta sleep. Often used together for comprehensive sleep and anti-aging support.
  • Can be used together. Growth hormone-releasing peptides benefit from better sleep quality. Use at different times of day for best results.
  • May enhance relaxation effects. Both have stress-modulating properties. Start with lower doses of each when combining.
  • No known negative interactions. BPC-157 focuses on gut and tissue healing while DSIP targets sleep—different systems.

With medications

  • !Benzodiazepines (Xanax, Valium, etc.) - Both enhance sleep/sedation—combining could cause excessive drowsiness. Consult your doctor before using together.
  • !Z-drugs (Ambien, Lunesta) - Similar concern as benzodiazepines—additive sedation effects. Don't combine without medical supervision.
  • Antidepressants - Generally considered safe, but effects on sleep architecture may vary. Inform your prescribing doctor.
  • Blood pressure medications - DSIP may have mild cardiovascular effects. Monitor blood pressure if using both. Likely fine but worth watching.

With supplements

  • Melatonin - Can be used together at low melatonin doses (0.5-1mg). Both support sleep through different pathways. Don't overdo the melatonin.
  • Magnesium - Excellent combination. Magnesium supports relaxation and may enhance DSIP's effects. Magnesium glycinate is particularly good for sleep.
  • L-theanine - Safe and potentially synergistic. L-theanine promotes relaxation without sedation, complementing DSIP's sleep-promoting effects.
  • Valerian root - May have additive sedation effects. If combining, start with lower doses of both to assess total effect.
  • High-dose GABA supplements - Theoretical overlap in mechanisms. Use caution and lower doses when combining. Monitor for excessive drowsiness.

Want the Full Picture?

View the complete DSIP (Delta Sleep-Inducing Peptide) research profile including mechanism of action, clinical studies, effectiveness timeline, and FAQ.

View Full DSIP (Delta Sleep-Inducing Peptide) Profile

Medical Disclaimer

DSIP (Delta Sleep-Inducing Peptide) is an investigational research compound not approved by the FDA for human therapeutic use. This information is for educational purposes only and should not be construed as medical advice. Always consult with a qualified healthcare provider before starting any new supplement or treatment protocol.

Last updated: 2/8/2026

DSIP (Delta Sleep-Inducing Peptide) Protocol — Complete Dosing & Administration Guide | Peptide Initiative