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Adipotide
Weight Management
AOD-9604
Weight Management
BPC-157
Healing & Recovery
Cagrilintide
Weight Management
CJC-1295
Growth Hormone
DSIP
Sleep & Recovery
Epithalon
Anti-Aging
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Anti-Aging
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Growth Hormone
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Hormone Support
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Growth Hormone
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Hormone Support
Melanotan-2
Cosmetic
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Metabolic
NAD+
Anti-Aging
Oxytocin Acetate
Hormone Support
PEG-MGF
Recovery
PNC-27
Cancer Research
PT-141
Sexual Health
Retatrutide
Weight Management
Selank
Cognitive
Semaglutide
Weight Management
Semax
Cognitive
Sermorelin
Growth Hormone
Snap-8
Cosmetic
SS-31
Mitochondrial
TB-500
Healing & Recovery
Tesamorelin
Growth Hormone
Thymosin Alpha-1
Immune
Tirzepatide
Weight Management
Total Peptides: 32
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Weight Management Protocol

SemaglutideComplete Dosing & Administration Guide

The revolutionary once-weekly GLP-1 medication that helps your body feel full faster and longer, leading to significant weight loss while also protecting your heart—FDA approved and backed by some of the largest clinical trials in obesity medicine history.

Dose Range

0.25-2.4mg

Frequency

Once weekly

Route

Subcutaneous injection

Cycle Length

Ongoing/indefinite

Dosing

How much
do I take?

Starting Dose

0.25 mg

Frequency

Once weekly

Duration

4 weeks

This starting dose is designed to let your body adjust to the medication and minimize nausea. You probably won't see much weight loss yet—that's normal. This phase is all about getting your GI system used to the medication.

Standard Dose

0.5-1.0 mg

Frequency

Once weekly

Duration

8+ weeks at each dose

After 4 weeks at 0.25mg, increase to 0.5mg. After another 4 weeks, move to 1.0mg. This gradual approach significantly reduces nausea and vomiting. Most people start seeing meaningful weight loss at the 0.5-1.0mg doses.

Advanced Dose

1.7-2.4 mg

Frequency

Once weekly

Duration

Ongoing/maintenance

The 2.4mg dose (Wegovy) is the FDA-approved dose for chronic weight management. Continue increasing by 0.5mg increments every 4 weeks until reaching 2.4mg. This is where the most significant weight loss occurs—averaging 15% or more of body weight in trials.

Timing

Best time to take

Choose any day of the week that works for your schedule and stick with it. Many people prefer Sunday or Monday to make it easy to remember. Take it at roughly the same time each week, though the exact hour doesn't matter much due to the long half-life.

With food?

Semaglutide injections can be taken with or without food—it doesn't affect absorption. However, because the medication slows stomach emptying, eating smaller meals will help reduce nausea. The oral version (Rybelsus) MUST be taken on an empty stomach with a small amount of water.

If stacking

Semaglutide is typically used as a standalone weight management therapy. If using with other diabetes medications, especially insulin or sulfonylureas, doses of those medications may need to be reduced to prevent low blood sugar. Always coordinate with your healthcare provider.

Adjusting Your Dose

Increase if

  • +You've tolerated the current dose for 4+ weeks without significant GI issues
  • +Weight loss has plateaued and you haven't reached target dose
  • +Blood sugar targets aren't being met (for diabetes patients)
  • +Your healthcare provider recommends progression based on your response

Decrease if

  • -Nausea, vomiting, or diarrhea are severe and persistent
  • -You're unable to eat enough to maintain basic nutrition
  • -You experience signs of dehydration from GI symptoms
  • -Side effects significantly impact your quality of life

Signs of right dose

  • Steady weight loss of 1-2 pounds per week
  • Feeling satisfied with smaller portions
  • Reduced food cravings and thoughts about food
  • Manageable or no GI side effects

Dosing Calculator

Calculate Your Exact Dose

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Step 1: Peptide Weight

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Select Weight

mg

Look for a number followed by 'mg' on the vial label (e.g., 5mg, 10mg)

Administration

How do I
use it?

Reconstitution

What you need

  • Pre-filled semaglutide pen (Ozempic or Wegovy)—no mixing required
  • Pen needles (usually provided or prescribed separately)
  • Alcohol swabs for injection site cleaning
  • Sharps container for safe needle disposal

Example

Semaglutide comes in pre-filled, pre-measured pens. For Wegovy: Starter pens contain set doses (0.25mg, 0.5mg, etc.) that you select. For Ozempic: The pen dial lets you select your dose (0.25mg, 0.5mg, 1mg, or 2mg). No calculations needed—the pen does the measuring for you.

Simply dial your prescribed dose on the pen. For example, if prescribed 0.5mg weekly, turn the dose selector to 0.5mg and inject. The pen window shows your selected dose. One pen typically contains multiple doses depending on your prescription.

Injection

Route

Subcutaneous injection (into the fatty tissue just under the skin)—quick, relatively painless, and can be self-administered at home

Best sites

  • Abdomen (stomach area)—at least 2 inches from belly button, most popular site
  • Front of thighs—middle section works best
  • Upper arm—outer area, may need assistance

Technique

  • 1.Remove pen cap and attach a new needle—never reuse needles
  • 2.Clean injection site with alcohol swab and let it air dry
  • 3.If using a new pen, prime it according to package instructions
  • 4.Dial your prescribed dose on the pen
  • 5.Pinch skin at injection site and insert needle straight in (90-degree angle)
  • 6.Press and hold the dose button until the dose counter shows 0
  • 7.Keep the button pressed and count slowly to 6 before removing needle
  • 8.Remove needle, dispose in sharps container, and replace pen cap
Full Injection Guide

Storage

Before reconstitution

Store new, unopened pens in the refrigerator at 36-46°F (2-8°C). Do not freeze—freezing destroys the medication. Keep in original carton to protect from light. If needed, unopened pens can be stored at room temperature (up to 86°F/30°C) for up to 28 days before first use.

After reconstitution

After first use, pens can be stored in the refrigerator or at room temperature up to 86°F (30°C). Use within 56 days of first injection. Always replace the pen cap after use to protect from light. Never store with needle attached.

Signs of degradation

  • Solution appears cloudy, discolored, or contains particles (should be clear and colorless)
  • Pen has been frozen or exposed to temperatures above 86°F (30°C) for extended periods
  • Solution looks yellow or has changed color
  • Pen is damaged or the dose selector doesn't click properly

Sample Daily Schedule

Same day each week (e.g., every Sunday morning)

As prescribed (0.25mg to 2.4mg depending on phase) injection

Site: Rotate between abdomen, thigh, and arm weekly

Pick a day you'll remember. Many people choose a weekend day so they can rest if they experience nausea. The injection takes less than 30 seconds once you get the hang of it. If you miss a dose by less than 5 days, take it as soon as you remember. If more than 5 days late, skip that dose and take the next one on your regular day.

Safety

Is it
safe?

Safety Profile

Semaglutide has been studied in over 20,000 participants across multiple large clinical trials, making it one of the most thoroughly researched weight loss medications available. The FDA approved it after rigorous review of safety data. While GI side effects are common (especially early on), serious side effects are rare. The SELECT trial showed it actually reduces cardiovascular events, adding a safety benefit beyond just weight loss.

The safety profile is supported by multiple Phase 3 trials including STEP 1-4 (weight loss) and SELECT (cardiovascular outcomes), with follow-up extending to nearly 4 years. Long-term studies continue, and post-marketing surveillance actively monitors for rare adverse events. The thyroid tumor signal seen in rodents has not been confirmed in humans after years of widespread use.

Common Side Effects

Experienced by some users

Nausea

The most frequently reported side effect, affecting about 44% of users at higher doses. Usually worst during the first few weeks and when increasing doses, then typically improves significantly.

Management: Eat smaller meals, avoid fatty or fried foods, stay upright after eating, and consider ginger or peppermint tea. The slow dose escalation protocol specifically minimizes this. If severe, your doctor may slow the dose increases.

Constipation or Diarrhea

GI motility changes are common as semaglutide slows stomach emptying. Some people experience constipation, others diarrhea, and some alternate between both.

Management: Stay well hydrated, increase fiber intake gradually, and maintain physical activity. Over-the-counter remedies like stool softeners (for constipation) or anti-diarrheals can help. Symptoms usually improve over time.

Decreased Appetite

This is actually how semaglutide works—it's a feature, not a bug. You'll naturally want to eat less and feel full faster. Some people find this dramatic enough that they forget to eat.

Management: While reduced appetite is the goal, make sure you're still eating regular nutritious meals. Focus on protein and vegetables at each meal. Skipping meals entirely can lead to muscle loss and nutritional deficiencies.

Vomiting

About 24% of users experience vomiting, especially during dose increases. Like nausea, it's typically transient and improves as your body adjusts.

Management: Eat bland foods, avoid lying down after meals, and stay hydrated. If vomiting is frequent, contact your healthcare provider—they may recommend staying at the current dose longer before increasing.

Less Common

  • Gallbladder Problems
  • Hair Loss (Telogen Effluvium)

These typically resolve with continued use or dose adjustment.

Stop and Seek Help If

  • ×Severe or persistent nausea and vomiting that prevents adequate nutrition despite dose adjustments
  • ×Signs of pancreatitis—severe abdominal pain radiating to the back
  • ×Allergic reaction—rash, itching, swelling, difficulty breathing
  • ×Signs of thyroid tumors—neck lump, hoarseness, trouble swallowing
  • ×Pregnancy or planning to become pregnant (stop at least 2 months before conception)
  • ×Severe kidney problems or dehydration from GI symptoms
  • ×Your healthcare provider recommends discontinuation

Semaglutide is a prescription medication that should only be started, adjusted, or stopped under medical supervision. This information is educational and does not replace professional medical advice. Stopping suddenly is generally safe, but discuss any changes with your healthcare provider.

Interactions

With other peptides

  • !Never combine GLP-1 medications—they work through the same mechanism and combining would increase side effects without additional benefit.
  • No direct interaction data exists. BPC-157 is sometimes used for GI healing, which could theoretically help with semaglutide's GI side effects, but this is speculative.
  • Limited interaction data. Some users combine them, but this should be done under medical supervision as both can affect glucose metabolism.

With medications

  • !Insulin - Semaglutide enhances insulin's blood sugar-lowering effect. Insulin doses typically need to be reduced 20-50% when starting semaglutide to prevent hypoglycemia. Work closely with your doctor.
  • !Sulfonylureas (glipizide, glyburide) - High risk of hypoglycemia when combined. Sulfonylurea dose usually needs reduction. Monitor blood sugar closely.
  • Oral Contraceptives - Semaglutide's effect on gastric emptying may affect absorption of oral medications. Consider taking birth control at a consistent time relative to meals, or discuss alternative contraception.
  • Warfarin - May affect warfarin absorption and metabolism. More frequent INR monitoring recommended when starting or changing semaglutide doses.
  • Levothyroxine - Take thyroid medication on an empty stomach as usual. Semaglutide shouldn't significantly affect absorption if taken at different times.

With supplements

  • Vitamin B12 - Long-term GLP-1 use may reduce B12 absorption. Consider B12 supplementation, especially if you've been on semaglutide for over a year.
  • Fiber Supplements - Can help manage constipation from semaglutide. Start slowly to avoid worsening GI symptoms. Psyllium husk is a good option.
  • Protein Supplements - Helpful for ensuring adequate protein intake when appetite is reduced. Aim for at least 60-80g protein daily to preserve muscle mass during weight loss.
  • Probiotics - May help with GI side effects. Some users find them helpful for digestive comfort during semaglutide therapy.

Want the Full Picture?

View the complete Semaglutide research profile including mechanism of action, clinical studies, effectiveness timeline, and FAQ.

View Full Semaglutide Profile

Medical Disclaimer

Semaglutide is an investigational research compound not approved by the FDA for human therapeutic use. This information is for educational purposes only and should not be construed as medical advice. Always consult with a qualified healthcare provider before starting any new supplement or treatment protocol.

Last updated: 2/8/2026

Semaglutide Protocol — Complete Dosing & Administration Guide | Peptide Initiative