Immune Protocol
NisinComplete Dosing & Administration Guide
FDA GRAS-approved lantibiotic (34 amino acids) produced by Lactococcus lactis with a dual antimicrobial mechanism — binds lipid II to block cell wall synthesis AND forms 2 nm transmembrane pores for rapid bacterial killing at MIC 0.5-4 μg/mL against gram-positive pathogens including MRSA and Listeria monocytogenes — the only antimicrobial peptide with over 50 years of documented safe human consumption
Dose Range
2.5-25mg/kg (food preservation; ADI 0.13 mg/kg BW)
Frequency
Once daily
Route
Oral (food-grade preservative, GRAS)
Cycle Length
Ongoing/indefinite
Dosing
How much
do I take?
Starting Dose
2.5-5 mg/kg (food preservation standard)
Standard food preservation concentration used in commercial dairy products, canned foods, and processed meats. At these levels, nisin is highly effective against Listeria monocytogenes and Clostridium species. This is the well-established, FDA GRAS application with decades of safety data. Optimal activity at pH 2-6.
Standard Dose
10-50 μg/mL (research topical application)
Research concentration for topical antimicrobial applications including wound care models, dental applications, and antimicrobial coating development. Effective against gram-positive skin and wound pathogens including MRSA at these concentrations. Can be combined with EDTA or polymyxin to extend gram-negative coverage.
Advanced Dose
50-200 μg/mL (research applications)
Higher research concentrations for anti-biofilm studies, anticancer research, and synergy studies with gram-negative-targeting agents. At 200 μg/mL combined with EDTA, nisin provides gram-negative coverage. Anticancer IC50 against MCF-7 is approximately 12 μg/mL. Medical supervision required for any non-food applications.
Timing
Best time to take
Take Nisin at the same time each day for consistent blood levels. Morning dosing with breakfast is often preferred, but follow your healthcare provider's specific instructions.
With food?
Nisin can typically be taken with or without food. Taking it with a light meal may help reduce any GI discomfort. Avoid taking with grapefruit juice or high-fat meals unless specifically directed.
If stacking
Nisin should be used as directed by your healthcare provider. If combining with other medications or supplements, discuss potential interactions with your provider. Avoid combining with compounds that have overlapping mechanisms unless specifically guided by a medical professional.
Adjusting Your Dose
Increase if
- +You've tolerated the current dose for the recommended period without significant side effects
- +Therapeutic goals haven't been met at the current dose level
- +Your healthcare provider recommends dose escalation based on your response
- +Lab work or clinical assessments support a higher dose
Decrease if
- -Side effects are bothersome or impacting daily life despite management strategies
- -You experience any signs of an adverse reaction
- -Lab results indicate the need for dose reduction
- -Your healthcare provider recommends a lower dose based on your response
Signs of right dose
- ✓Therapeutic goals being met with minimal side effects
- ✓Stable and consistent response to treatment
- ✓Lab values or clinical markers trending in the right direction
- ✓Good tolerance with manageable or absent side effects
Dosing Calculator
Calculate Your Exact Dose
Step 1: Peptide Weight
Find the weight printed on your peptide vial label
Look here!
The peptide weight is printed on the label
Look here!
The weight is on the label
Administration
How do I
use it?
Reconstitution
What you need
- •Nisin in its prescribed form
- •Clean, dry storage container
- •Measuring device if applicable (oral syringe, measuring cup)
- •Calendar or reminder app for dosing schedule
Injection
Route
Nisin is administered Oral (food-grade preservative, GRAS)—no injection required
Best sites
- •Not applicable—this is not an injectable formulation
Technique
- 1.Follow the specific administration instructions for your Nisin formulation
- 2.Take or apply as directed by your healthcare provider
- 3.Store properly between uses according to package instructions
Storage
Signs of degradation
Sample Daily Schedule
Safety
Is it
safe?
Safety Profile
Nisin holds FDA GRAS status since 1988 with over 50 years of documented safe human consumption, making it the only antimicrobial peptide with this comprehensive long-term safety record. Oral LD50 of 174 mg/kg in mice (comparable to table salt) demonstrates extraordinary safety margin. Acceptable daily intake established at 0.13 mg/kg body weight with no reported serious adverse events at this or higher levels. Primary risk is milk protein contamination during production, requiring screening for lactose-intolerant or milk-allergic individuals.
FDA GRAS designation based on 50+ years of food-industry safety data with millions of exposed consumers globally. Bacterial production method prevents synthetic impurities; stability through pasteurization and food processing verified via microbiological and mass spectrometry analysis. Animal toxicity studies and human safety monitoring across multiple continents show no systemic toxicity, genotoxicity, or carcinogenic potential. European Food Safety Authority also designated as safe food additive (E234).
Common Side Effects
Experienced by some users
No significant effects at dietary levels
At FDA GRAS concentrations used in food preservation (ADI 0.13 mg/kg BW), nisin has no documented side effects across 50+ years of global dietary consumption by millions of people.
Management: No management needed. Nisin at dietary levels is one of the most well-documented safe food additives in history.
Mild GI discomfort at research doses
At oral doses significantly exceeding normal dietary exposure in research settings, mild gastrointestinal discomfort (nausea, mild cramping) may occur.
Management: Take with food. Reduce dose if symptoms persist. GI effects are typically transient and dose-dependent.
Local irritation (topical use)
Mild local irritation, redness, or warmth at topical application sites in wound care or dental research settings.
Management: Generally self-limiting. Reduce concentration if irritation is excessive. Nisin is most stable and active at acidic pH, which may contribute to local irritation.
Less Common
- •Milk protein allergic reaction
- •Gram-negative overgrowth
These typically resolve with continued use or dose adjustment.
Stop and Seek Help If
- ×Severe or worsening side effects that don't improve with dose adjustment or supportive care
- ×Signs of an allergic reaction—rash, hives, swelling, or difficulty breathing
- ×Your healthcare provider recommends discontinuation based on your clinical response
- ×Development of any new medical condition that may be contraindicated with Nisin
- ×Pregnancy or planning to become pregnant (unless specifically approved for use during pregnancy)
- ×Abnormal lab results or clinical markers that suggest adverse effects
Nisin should only be started, adjusted, or discontinued under medical supervision. This information is for educational purposes only and does not replace professional medical advice. Never stop a prescribed treatment without consulting your healthcare provider first, as abrupt discontinuation may have consequences.
Interactions
With other peptides
- ✓May be used together under medical guidance.
- ✓May be used together under medical guidance.
- ✓May be used together under medical guidance.
With medications
- !Alkaline formulations (pH >7) — nisin activity and stability decrease significantly above neutral pH, with degradation accelerating at alkaline conditions - Use with caution—discuss with your healthcare provider.
- !Proteolytic enzymes (trypsin, chymotrypsin at high concentrations) — while lanthionine bridges provide some resistance, extensive proteolysis can reduce activity - Use with caution—discuss with your healthcare provider.
- !High-fat food matrices without emulsification — nisin may partition into fat phase, reducing aqueous concentration and antimicrobial activity - Use with caution—discuss with your healthcare provider.
With supplements
- ✓Multivitamins - Generally safe to take alongside Nisin. Space doses apart if taking oral formulations to ensure optimal absorption.
- ✓Electrolyte supplements - Helpful if experiencing any GI side effects that could lead to dehydration. Safe to combine.
Want the Full Picture?
View the complete Nisin research profile including mechanism of action, clinical studies, effectiveness timeline, and FAQ.
View Full Nisin Profile