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Hormone Support Protocol

Human Chorionic Gonadotropin (HCG)Complete Dosing & Administration Guide

A powerful hormone that mimics luteinizing hormone (LH) to stimulate your body's natural testosterone production while keeping your fertility intact—like having your cake and eating it too for men on hormone therapy.

Dose Range

250-2000IU

Frequency

Twice weekly

Route

Subcutaneous injection

Cycle Length

Ongoing/indefinite

Dosing

How much
do I take?

Starting Dose

250-500 IU

Frequency

Twice weekly

Duration

Ongoing with TRT

This is a conservative starting point for men using HCG alongside testosterone therapy. The goal is to maintain testicular function and fertility without overstimulating estrogen production. Start low and adjust based on lab work and how you feel.

Standard Dose

500-1000 IU

Frequency

Twice weekly

Duration

Ongoing with TRT or standalone

The most commonly prescribed range for men on TRT who want to maintain testicular size and fertility. Many clinics use 500 IU two to three times per week as their standard protocol. Monitor estrogen levels as HCG can increase aromatization.

Advanced Dose

1000-2000 IU

Frequency

Two to three times weekly

Duration

12-16 weeks for restart protocols

Higher doses are typically used for fertility induction in hypogonadotropic hypogonadism or as part of post-cycle therapy after anabolic steroid use. These protocols often include FSH as well. Requires careful medical supervision and monitoring of estrogen and testosterone levels.

Timing

Best time to take

Most men inject HCG on set days—for example, Monday and Thursday, or Sunday and Wednesday. Time of day doesn't matter much, but consistency helps you remember. Some prefer morning injections.

With food?

HCG can be taken regardless of food timing. Absorption is not affected by meals since it's injected, not taken orally.

If stacking

When using with testosterone, inject HCG on the same days or alternate days—both approaches work. If using an aromatase inhibitor, take it as prescribed to manage any estrogen increase from HCG.

Adjusting Your Dose

Increase if

  • +Testicular volume continues to decrease despite current dose
  • +Sperm count remains suppressed when fertility is the goal
  • +Your doctor determines testosterone response is inadequate
  • +You're transitioning to a fertility-focused protocol

Decrease if

  • -Estrogen levels rise too high (confirmed by blood work)
  • -You experience significant water retention or bloating
  • -Gynecomastia symptoms develop (tender or swelling breast tissue)
  • -Mood swings or irritability become problematic

Signs of right dose

  • Testicles maintain normal size and firmness
  • Hormone levels (testosterone, estrogen, LH) are in healthy ranges
  • Sperm production maintained or improved (if monitored)
  • No significant side effects like gynecomastia or water retention

Dosing Calculator

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Administration

How do I
use it?

Reconstitution

What you need

  • Bacteriostatic water (BAC water)—the preservative allows multiple uses
  • Insulin syringes (29-31 gauge)—thin needles for comfortable injections
  • Alcohol swabs for cleaning vial tops and injection sites
  • Your HCG powder vial (typically 5,000 IU or 10,000 IU)

Example

If you have a 5,000 IU vial and add 2.5mL of BAC water, you get a concentration of 2,000 IU/mL. So every 0.25mL (25 units on an insulin syringe) equals 500 IU of HCG.

For a 500 IU dose at 2,000 IU/mL concentration: draw 0.25mL (25 units on a standard insulin syringe). For 1,000 IU: draw 0.5mL (50 units).

Injection

Route

Subcutaneous injection (just under the skin) or intramuscular injection—subcutaneous is easier for self-administration and equally effective

Best sites

  • Belly fat area (about 2 inches away from your belly button)
  • Front or outer thigh (middle section)
  • Upper outer buttock area (for IM injections)

Technique

  • 1.Wash your hands thoroughly with soap and water
  • 2.Clean the injection site with an alcohol swab and let it air dry
  • 3.For subcutaneous: pinch about an inch of skin to create a fold
  • 4.Insert the needle at a 45-90 degree angle (45 if you're lean, 90 if you have more tissue)
  • 5.Push the plunger slowly and steadily over 5-10 seconds
  • 6.Wait a few seconds before removing the needle
  • 7.Apply light pressure with a clean swab if needed—don't rub
Full Injection Guide

Storage

Before reconstitution

Keep your HCG powder in the refrigerator (36-46°F / 2-8°C). While some products claim room temperature stability, refrigeration extends shelf life. Store in the original sealed vial away from light. Properly stored powder remains stable for 1-2 years.

After reconstitution

Once mixed with bacteriostatic water, refrigerate at 36-46°F (2-8°C). Never freeze the reconstituted solution—freezing damages the protein structure and destroys the hormone. Keep away from light. Use within 30-60 days depending on your provider's guidance.

Signs of degradation

  • Cloudy or hazy appearance (should be crystal clear)
  • Visible particles floating or settled at the bottom
  • Color changes—any discoloration means discard it
  • Clumping or precipitation that doesn't dissolve when gently swirled

Sample Daily Schedule

Morning (or whenever convenient)

250-500 IU injection

Site: Rotate between belly and thigh

Standard twice-weekly protocol: inject Monday and Thursday, or Sunday and Wednesday. Pair with your TRT injections or on alternate days—both approaches are effective. Most men use HCG continuously alongside testosterone therapy.

Safety

Is it
safe?

Safety Profile

HCG has been used medically for over 80 years with a well-established safety profile. When used appropriately for male hypogonadism or fertility preservation, side effects are generally mild and manageable. The most common issues relate to elevated estrogen from increased testosterone aromatization, which can be managed with aromatase inhibitors if needed. Serious side effects are rare when HCG is used under medical supervision.

HCG is FDA-approved for specific indications including hypogonadotropic hypogonadism in males. There's substantial clinical evidence from decades of use in fertility medicine and more recent research on its role in TRT protocols. Large real-world studies have confirmed its safety and effectiveness when properly monitored.

Common Side Effects

Experienced by some users

Injection site reactions

Mild pain, redness, or swelling at the injection site is normal and typically resolves within a day or two.

Management: Rotate injection sites regularly. Use proper technique and clean supplies. A cold compress can help if there's swelling.

Headache

Some men experience headaches, especially when starting HCG therapy. This usually improves after the first few weeks.

Management: Stay well-hydrated and use over-the-counter pain relievers if needed. If persistent, discuss with your doctor.

Water retention

Mild fluid retention can occur, causing slight bloating or puffiness. This is related to hormonal changes.

Management: Reduce sodium intake, stay hydrated, and exercise regularly. Usually mild and manageable.

Less Common

  • Gynecomastia
  • Mood changes
  • Acne

These typically resolve with continued use or dose adjustment.

Stop and Seek Help If

  • ×Signs of allergic reaction—rash, hives, swelling, difficulty breathing (stop immediately, seek emergency care)
  • ×Significant gynecomastia development that doesn't respond to estrogen management
  • ×Blood clot symptoms—sudden leg pain/swelling, chest pain, shortness of breath (stop immediately, seek emergency care)
  • ×Your fertility or TRT goals have been achieved and your doctor recommends discontinuation
  • ×Unacceptable side effects that don't improve with dose adjustments
  • ×Development of hormone-sensitive conditions

HCG is a prescription medication and should only be used under medical supervision. Never start, stop, or adjust your dosing without consulting your healthcare provider. This information is for educational purposes only—not medical advice.

Interactions

With other peptides

  • Both stimulate the reproductive axis through different mechanisms. Can be used together in some protocols but monitor hormone levels carefully.
  • Experimental combination—both affect gonadotropin release. Limited data on concurrent use.
  • No known interaction. Different mechanisms of action.

With medications

  • Testosterone (TRT) - Commonly used together. HCG helps maintain testicular function during TRT. This is a standard protocol.
  • Clomiphene citrate - Often combined for fertility protocols or PCT. Both stimulate testosterone through different pathways.
  • Aromatase inhibitors (Anastrozole) - May be needed to control estrogen that rises with HCG use. Monitor levels and dose appropriately.
  • Blood thinners (Warfarin, etc.) - May increase bruising at injection sites. Monitor INR closely as hormone changes can affect clotting.
  • Thyroid medications - HCG has weak TSH-like activity. Monitor thyroid function if on thyroid medication.

With supplements

  • DHEA - Both affect hormone levels. Use together cautiously and monitor hormone panels.
  • Zinc - Zinc supports testosterone production and is safe to take with HCG.
  • Vitamin D - Important for hormone health. Safe and potentially complementary.
  • DIM (Diindolylmethane) - May help with estrogen metabolism. Can be used alongside HCG.

Want the Full Picture?

View the complete Human Chorionic Gonadotropin (HCG) research profile including mechanism of action, clinical studies, effectiveness timeline, and FAQ.

View Full Human Chorionic Gonadotropin (HCG) Profile

Medical Disclaimer

Human Chorionic Gonadotropin (HCG) is an investigational research compound not approved by the FDA for human therapeutic use. This information is for educational purposes only and should not be construed as medical advice. Always consult with a qualified healthcare provider before starting any new supplement or treatment protocol.

Last updated: 2/8/2026

Human Chorionic Gonadotropin (HCG) Protocol — Complete Dosing & Administration Guide | Peptide Initiative