Kisspeptin

Reconstitution:

• Add 2-3 mL bacteriostatic water to 10mg vial
• This creates 3.33-5 mg/mL concentration
• Gently swirl - do not shake vigorously

Drawing the Dose:

• Use insulin syringe for accuracy
• 100 mcg dose = approximately 30 units on U-100 syringe
• Double-check dose calculations

Injection Technique:

1. Clean injection site with alcohol swab
2. Pinch skin and insert needle at 45-90 degree angle
3. Inject slowly over 5-10 seconds
4. Hold for 5 seconds before withdrawing
5. Apply gentle pressure with gauze

Rotation Sites:

• Alternate between abdomen, thigh, and upper arm
• Keep injection log to track sites

Avoid Daily Dosing:

• Daily use causes rapid desensitization
• Receptor downregulation occurs within 24 hours
• Maintain 3-4 day minimum between doses

Optimal Timing:

• Morning injections may align better with natural hormone rhythms
• Consistency is more important than specific time

Missed Doses:

• Skip if less than 3 days until next scheduled dose
• Never double up doses
• Resume regular schedule

Expected Reactions:

• Mild warmth or flushing for 10-30 minutes
• Slight increase in heart rate (normal response)
• These indicate the medication is working

Managing Side Effects:

• Stay hydrated before and after injection
• Sit or lie down if dizzy
• Apply ice to injection site if irritated

Seek Medical Attention If:

• Severe allergic reaction (rare)
• Persistent headaches or vision changes
• Unusual changes in menstrual bleeding
• Signs of overstimulation (in fertility treatment)

Optimize Metabolic Health:

• Maintain healthy body weight (BMI 20-25 optimal)
• Regular exercise improves hormone sensitivity
• Adequate sleep (7-9 hours) supports reproductive axis

Nutritional Support:

• Ensure adequate leptin levels (avoid extreme dieting)
• Zinc and vitamin D support hormone production
• Omega-3 fatty acids may enhance response

Stress Management:

• Chronic stress suppresses reproductive axis
• Consider meditation or yoga
• Cortisol can interfere with kisspeptin action

Timing with Natural Cycles:

• Women: Consider cycle phase for optimal response
• Men: Morning injections may work better
• Avoid during illness or extreme stress

Initial Assessment:

• Baseline hormone panels before starting
• Review of reproductive history
• Discussion of treatment goals

Regular Monitoring:

• Hormone levels every 4-6 weeks initially
• Adjust dose based on response
• Monitor for signs of desensitization

Long-term Follow-up:

• Assess need for continued treatment
• Consider treatment breaks if needed
• Watch for changes in effectiveness